A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR

EMBO Reports

Volumn 4, Issue 2, 2003, Pages 159-165

  Neddermann, Petra ^a   Gargioli, Cesare ^a   Muraglia, Ester ^a   Sambucini, Sonia ^a   Bonelli, Fabio ^a   De Francesco, Raffaele ^a   Cortese, Riccardo ^a  

^a IRBM   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RECEPTOR; HYBRID PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LIGAND; N (3 OXO OCTANOYL)HOMOSERINE LACTONE; PROTEIN TRAR; SIGNAL PEPTIDE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; CHIMERIC PROTEIN; DNA; N (3 OXODODECANOYL)HOMOSERINE LACTONE; TRANSCRIPTION FACTOR RELA; TRANSCRIPTION FACTOR TRAR;

ANIMAL CELL; ARTICLE; BACTERIAL COUNT; CELL COMMUNICATION; CELL LINE; CHIMERA; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA SEQUENCE; GENE EXPRESSION SYSTEM; GENE INDUCTION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; MICROBIAL ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; RHIZOBIUM RADIOBACTER; TRANSCRIPTION REGULATION; AGROBACTERIUM TUMEFACIENS; BIOTECHNOLOGY; EUKARYOTE; MAMMAL CELL; MOLECULAR RECOGNITION; PROMOTER REGION; PROTEIN DNA BINDING; QUORUM SENSING;

BACTERIAL PROTEINS; DNA; GENE EXPRESSION; GENE EXPRESSION REGULATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; TRANSCRIPTION, GENETIC;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ANIMALIA; BACTERIA (MICROORGANISMS); EUKARYOTA; MAMMALIA; NEGIBACTERIA;

EID: 0037298235     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.embor.embor734     Document Type: Article

References (21)

1. Baron, U., Gossen, M. & Bujard, H. (1997) Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential. Nucleic Acids Res., 25, 2723-2729.
2. Burcin, M.M., Schiedner, G., Kochanek, S., Tsai, S.Y. & O'Malley, B.W. (1999) Adenovirus-mediated regulable target gene expression in vivo. Proc. Natl Acad. Sci. USA, 96, 355-360.
3. Chastain, M. et al. (2001) Antigen levels and antibody titers after DNA vaccination. J. Pharm. Sci., 90, 474-484.
4. Devine, J.H., Shadel, G.S. & Baldwin, T.O. (1989) Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. Proc. Natl Acad. Sci. USA, 86, 5688-5692.
5. Fuqua, W.C. & Winans, S.C. (1994) A LuxR-Luxl type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J. Bacteriol., 176, 2796-2806.
6. Fuqua, C. & Winans, S.C. (1996) Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J. Bacteriol., 178, 435-440.
7. Gardiner, S.M., Chhabra, S.R. & Harty, C. (2001) Haemodynamic effects of the bacterial quorum-sensing signal molecule N-(3-oxododecanoyl)-L-homoserine lactone, in conscious, normal and endotoxaemic rats. Infect. Immun., 67, 5076-5082.
8. Gossen, M. & Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl Acad. Sci. USA, 89, 5547-5551.
9. Harvey, D.M. & Caskey, C.T. (1998) Inducible control of gene expression: prospects for gene therapy. Curr. Opin. Chem. Biol., 2, 512-518.
10. Hwang, I. et al. (1994) Tral, a Luxl homologue, is responsible for production of conjugation factor, the Ti Plasmid N-acylhomoserine lactone autoinducer. Proc. Natl Acad. Sci. USA, 91, 4639-4643.
11. Miller, M.B. & Bassler, B.L. (2001) Quorum-sensing in bacteria. Annu. Rev. Microbiol., 55, 165-199.
12. Piper, K.R., Beck von Bodman, S. & Farrand, S.K. (1993) Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature, 362, 448-450.
13. Qin, Y. et al. (2000) Quorum-sensing signal binding results in dimerization of TraR and its release from membranes into the cytoplasm. EMBO J., 19, 5212-5221.
14. Saez, E., No, D., West, A. & Evans, R.M. (1997) Inducible gene expression in mammalian cells and transgenic mice. Curr. Opin. Biotech., 8, 608-616.
15. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
16. Smith, R.S. et al. (2001) IL-8 production in human lung fibroblasts and epithelial cells activated by the Pseudomonas autoinducer N-3-oxododecanoyl homoserine lactone is transcriptionally regulated by NF-κB and activator protein-2. J. Immunol., 167, 366-374.
17. Vannini, A. et al. (2002) The crystal structure of the quorum-sensing protein TraR bound to its autoinducer and target DNA. EMBO J., 21, 1-9.
18. Yates, E.A. et al. (2002) N-acylhomoserine lactones undergo lactonolysis in a pH-, temperature-, and acyl chain length-dependent manner during growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa. Infect. Immunol., 70, 5635-5646.
19. Zhang, L., Murphy, P.J., Kerr, A. & Tate, M.E. (1993) Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Nature, 362, 446-448.
20. Zhang, R.G. et al. (2002) Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. Nature, 417, 971-974.
21. Zhu, J. & Winans, S. C. (2001) The quorum-sensing transcriptional regulator TraR requires its cognate signaling ligand for protein folding, protease resistance, and dimerization. Proc. Natl Acad. Sci USA, 98, 1507-1512.