Development of a 5′-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue

Journal of Applied Microbiology

Volumn 94, Issue 4, 2003, Pages 587-594

  Perelle, Sylvie ^a   Dilasser, F ^a   Grout, J ^a   Fach, P ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

Author keywords

'O island 29'; Real time PCR; STEC O145

Indexed keywords

ASSAYS; ESCHERICHIA COLI; GENE ENCODING; POLYMERASE CHAIN REACTION;

'O-ISLAND 29'; E. COLI; PCR ASSAY; REAL-TIME PCR; REAL-TIME PCR ASSAY; SEQUENCE DIVERGENCES; SEROGROUPS; SEROTYPING; SHIGA TOXIN-PRODUCING ESCHERICHIA COLI; STEC O145;

DNA SEQUENCES;

NUCLEASE; SHIGA TOXIN; BACTERIAL DNA;

BACTERIUM;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI O145; ESCHERICHIA COLI O157; GENE LOCUS; GENETIC VARIABILITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SEROTYPE; STRAIN DIFFERENCE; STRAIN IDENTIFICATION; TIME; AMINO ACID SEQUENCE; BIOSYNTHESIS; CLASSIFICATION; EVALUATION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

AMINO ACID SEQUENCE; BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; DNA, BACTERIAL; ESCHERICHIA COLI O157; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SHIGA TOXIN;

EID: 0037268365     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2003.01872.x     Document Type: Article

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