Evaluation of a 5′-nuclease (TaqMan) assay for the detection of virulent strains of Yersinia enterocolitica in raw meat and tofu samples

Journal of Food Protection

Volumn 64, Issue 3, 2001, Pages 355-360

  Vishnubhatla, A ^a   Oberst, R D ^a   Fung, D Y C ^a   Wonglumsom, W ^a   Hays, M P ^a   Nagaraja, T G ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; BACTERIAL ENZYME; COLONY FORMING UNIT; CRYSTAL VIOLET; ENTEROTOXIN; ENZYME ASSAY; FOOD CONTAMINATION; FOOD PROCESSING; INOCULATION; MICROBIAL CONTAMINATION; MICROORGANISM DETECTION; PATHOGENICITY; PORK; UNITED STATES;

BACTERIA (MICROORGANISMS); GLYCINE MAX; VIOLA (ANGIOSPERM); YERSINIA ENTEROCOLITICA;

DEOXYRIBONUCLEASE; FLUORESCENT DYE; TAQ POLYMERASE;

ANIMAL; ARTICLE; BACTERIAL COUNT; CATTLE; CHEMISTRY; COMPARATIVE STUDY; CULTURE MEDIUM; EVALUATION; FOOD CONTAMINATION; GENETICS; ISOLATION AND PURIFICATION; MEAT; METABOLISM; MICROBIOLOGY; PATHOGENICITY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEAN; SWINE; VIRULENCE; YERSINIA ENTEROCOLITICA;

ANIMALS; CATTLE; COLONY COUNT, MICROBIAL; CULTURE MEDIA; DEOXYRIBONUCLEASES; FLUORESCENT DYES; FOOD CONTAMINATION; MEAT; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEANS; SWINE; TAQ POLYMERASE; VIRULENCE; YERSINIA ENTEROCOLITICA;

EID: 0034745351     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-64.3.355     Document Type: Article

References (35)

1. Yersinia enterocolitica: A panoramic view of a charismatic organism
2. Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes
3. Molecular diagnostics for dairy-borne pathogens
4. Direct detection and isolation of plasmid-bearing virulent serotypes of Yersinia enterocolitica from various foods
5. Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples
6. The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities
7. Yersinia enterocolitica in Danish pigs
8. Yersinia pathogenicity factors
9. Isolation of virulent Yersinia enterocolitica from porcine tongues
10. Detection of pathogenic Yersinia enterocolitica in milk and pork using a DIG-labeled probe targeted against the yst gene
11. Multiplex riboprobes for the detection of virulent Yersinia enterocolitica and simple methods for their preparation
12. Rapid and specific fluorogenic PCR-based system for the detection of Shiga-like toxins I and II producing E. coli
13. Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermos aquaticus DNA polymerase
14. Development of a highly specific assay for rapid identification of pathogenic strains of Yersinia enterocolitica based on PCR amplification of Yersinia heat-stable enterotoxin gene (yst)
15. Polymerase chain reaction-gene probe detection system specific for pathogenic strains of Yersinia enterocolitica
16. Yersinia enterocolitica in food hygiene
17. A synthetic oligonucleotide probe and a cloned polynucleotide probe based on the yopA gene for the detection and enumeration of virulent Yersinia enterocolitica
18. Evaluation of TaqMan PCR assay for detecting Salmonella in raw meat and shrimp
19. Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction and digoxigenin-labeled polynucleotide probes
20. Yersinia enterocolitica O:3 infections in infants and children, associated with the household preparation of chitterlings
21. Allelic discrimination by nick-translation PCR with fluorogenic probes
22. Yersinia spp. and numbers, with particular reference to Y. enterocolitica bio/serotypes, occurring on Irish meat and meat products, and the influence of alkali treatment on their isolation
23. Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases
24. Detection of Salmonella in food using a fluorogenic 5′ nuclease assay
25. Detection and identification of Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica by an improved polymerase chain reaction method
26. Enumeration of Yersinia enterocolitica O:3 from the porcine oral cavity, and its occurrence on cut surfaces of pig carcasses and the environment in a slaughterhouse
27. PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease TaqMan assay
28. Yersinia enterocolitica
29. Execution of ELISA technique
30. Effect of sample handling on microbial limits laid down in standards
31. Development of a rapid 5′ nuclease assay for the detection of virulent strains of Yersinia enterocolitica
32. Yersinia enterocolitica
33. A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef
34. Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction