Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infection

Microbiology

Volumn 149, Issue 1, 2003, Pages 167-176

  Struve, Carsten ^a   Forestier, Christiane ^b   Krogfelt, Karen A ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b UNIVERSITÉ CLERMONT AUVERGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; VIRULENCE FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; BACTERIAL COLONIZATION; BACTERIAL GENE; BACTERIAL MEMBRANE; BACTERIAL PNEUMONIA; BACTERIAL TRANSMISSION; BIOASSAY; CONTROLLED STUDY; DNA HYBRIDIZATION; FEMALE; GASTROINTESTINAL TRACT; GENE AMPLIFICATION; GENE FUNCTION; GENE IDENTIFICATION; GENE INSERTION; GENE LIBRARY; GENE MUTATION; GENETIC CODE; GENETIC SCREENING; GRAM NEGATIVE INFECTION; IMMUNE DEFICIENCY; INOCULATION; INTESTINE; KLEBSIELLA PNEUMONIAE; METHODOLOGY; MICROBIAL ATTENUATION; MOLECULAR PROBE; MOUSE; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESERVOIR; REVIEW; TRANSPOSON; URINARY TRACT INFECTION;

ANIMALS; ANIMALS, OUTBRED STRAINS; BACTERIAL PROTEINS; DISEASE MODELS, ANIMAL; DNA TRANSPOSABLE ELEMENTS; FEMALE; GENES, ESSENTIAL; HUMANS; INTESTINES; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; MICE; MUTAGENESIS, INSERTIONAL; MUTATION; POLYMERASE CHAIN REACTION; URINARY TRACT INFECTIONS; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE; NEGIBACTERIA;

EID: 0037256321     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.25833-0     Document Type: Review

References (54)

1. Andrianopoulos, K., Wang, L. & Reeves, P. R. (1998). Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12. J Bacteriol 180, 998-1001.
2. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. E., Seidman, J. G., Smith, J. A. & Struhl, K. (1995). Current Protocols in Molecular Biology. New York: Wiley.
3. Behrens, S., Maier, R., de Cock, H., Schmid, F. X. & Gross, C. A. (2001). The SurA periplasmic PPIase lacking its parvulin domains functions in vivo and has chaperone activity. EMBO J 20, 285-294.
4. Benedi, V. J., Vivanco, F. & Tomas, J. M. (1998). Complement activation in Klebsiella pneumoniae. Rev Med Microbiol 9, 69-77.
5. Bispham, J., Tripathi, B. N., Watson, P. R. & Wallis, T.-S. (2001). Salmonella pathogenicity island 2 influences both systemic salmonellosis and Salmonella-induced enteritis in calves. Infect Immun 69, 367-377.
6. Camprubi, S., Merino, S., Benedi, V. J. & Tomas, J. M. (1993a). The role of the O-antigen lipopolysaccharide and capsule on an experimental Klebsiella pneumoniae infection of the rat urinary tract. FEMS Microbiol Lett 111, 9-13.
7. Camprubi, S., Merino, S., Guillot, J. F. & Tomas, J. M. (1 993b). The role of the O-antigen lipopolysaccharide on the colonization in vivo of the germfree chicken gut by Klebsiella pneumoniae. Microb Pathog 14, 433-440.
8. Chakraborty, A. K., Friebolin, H., Niemann, H. & Stirm, S. (1977). Primary structure of the Klebsiella serotype 16 capsular polysaccharide. Carbohydr Res 59, 525-530.
9. Clarke, B. R., Bronner, D., Keenleyside, W. J., Severn, W. B., Richards, J. C. & Whitfield, C. (1995). Role of Rfe and RfbF in the initiation of biosynthesis of D-galactan I, the lipopolysaccharide O-antigen from Klebsiella pneumoniae serotype O1. J Bacteriol 177, 5411-5418.
10. Connell, H., Agace, W., Klemm, P., Schembri, M., Marild, S. & Svanborg, C. (1996). Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract. Proc Natl Acad Sci U S A 93, 9827-9832.
11. Cortés, G., Borrell, N., de Astorza, B., Gomez, C., Sauleda, J. & Alberti, S. (2002). Molecular analysis of the contribution of the capsular polysaccharide and lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumonia. Infect Immun 70, 2583-2590.
12. Coulter, S. N., Schwan, W. R., Ng, E. Y. & 7 other authors (1998). Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments. Mol Microbiol 30, 393-404.
13. Favre-Bonte, S., Licht, T. R., Forestier, C. & Krogfelt, K. A. (1999). Klebsiella pneumoniae capsule expression is necessary for colonization of large intestine of streptomycin-treated mice. Infect Immun 67, 6152-6156.
14. Fuller, T. E., Kennedy, M. J. & Lowery, D. E. (2000). Identification of Pasteurella multocida virulence genes in a septicemic mouse model using signature-tagged mutagenesis. Microb Pathog 29, 25-38.
15. Gunsalus, R. P. & Park, S. J. (1994). Aerobic-anaerobic gene regulation in Escherichia coli: control by the ArcAB and Fnr regulons. Res Microbiol 145, 437-450.
16. Hansen, D. S., Gottschau, A. & Kolmos, H. J. (1998). Epidemiology of Klebsiella bacteraemia: a case control study using Escherichia coli bacteraemia as control. J Hosp Infect 38, 119-132.
17. Hensel, M., Shea, J. E., Gleeson, C., Jones, M. D., Dalton, E. & Holden, D. W. (1995). Simultaneous identification of bacterial virulence genes by negative selection. Science 269, 400-403.
18. Herrero, M., de Lorenzo, V. & Timmis, K. N. (1990). Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol 172, 6557-6567.
19. Hitchcock, P. J. & Brown, T. M. (1983). Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154, 269-277.
20. Hvidberg, H., Struve, C., Krogfelt, K. A., Christensen, N., Rasmussen, S. N. & Frimodt-Moller, N. (2000). Developmnt of a long-term ascending urinary tract infection mouse model for antibiotic treatment studies. Antimicrob Agents Chemother 44, 156-163.
21. Keane, W. F. & Freedman, L. R. (1967). Experimental pyelonephritis. XIV. Pyelonephritis in normal mice produced by inoculation of E. coli into the bladder lumen during water diuresis. Yale J Biol Med 40, 231-237.
22. Lai, Y. C., Pang, H. L. & Chang, H. Y. (2001). Identification of genes induced in vivo during Klebsiella pneumoniae CG43 infection. Infect Immun 69, 7140-7145.
23. Langermann, S., Palaszynski, S., Barnhart, M. & 8 other authors (1997). Prevention of mucosal Escherichia coli infection by FimH-adhesin-based systemic vaccination. Science 276, 607-611.
24. Lau, G. W., Haataja, S., Lonetto, M., Kensit, S. E., Marra, A., Bryant, A. P., McDevitt, D., Morrison, D. A. & Holden, D. W. (2001). A functional genomic analysis of type 3 Streptococcus pneumoniae virulence. Mol Microbiol 40, 555-571.
25. Lazar, S. W. & Kolter, R. (1996). SurA assists the folding of Escherichia coli outer membrane proteins. J Bacteriol 178, 1770-1773.
26. Licht, T. R., Krogfelt, K. A., Cohen, P. S., Poulsen, L. K., Urbance, J. & Molin, S. (1996). Role of lipopolysaccharide in colonization of the mouse intestine by Salmonella typhimurium studied by in situ hybridization. Infect Immun 64, 3811-3817.
27. Maroncle, N., Balestrino, D., Rich, C. & Forestier, C. (2002). Identification of Klebsiella pneumoniae genes involved in intestinal colonization and adhesion using signature-tagged mutagenesis. Infect Immun 70, 4729-4734.
28. Martindale, J., Stroud, D., Moxon, E. R. & Tang, C. M. (2000). Genetic analysis of Escherichia coli K1 gastrointestinal colonization. Mol Microbiol 37, 1293-1305.
29. Mei, J. M., Nourbakhsh, F., Ford, C. W. & Holden, D. W. (1997). Identification of Staphylococcus aureus virulence genes in a murine model of bacteraemia using signature-tagged mutagenesis. Mol Microbiol 26, 399-407.
30. Merino, S., Rubires, X., Aguilar, A. & Tomas, J. M. (1997). The role of O1-antigen in the adhesion to uroepithelial cells of Klebsiella pneumoniae grown in urine. Microb Pathog 23, 49-53.
31. Merino, S., Altarriba, M., Izquierdo, L., Nogueras, M. M., Regue, M. & Tomas, J. M. (2000). Cloning and sequencing of the Klebsiella pneumoniae O5 wb gene cluster and its role in pathogenesis. Infect Immun 68, 2435-2440.
32. Merrell, D. D., Hava, D. L. & Camilli, A. (2002). Identification of novel factors involved in colonization and acid tolerance of Vibrio cholerae. Mol Microbiol 43, 1471-1491.
33. Miller, V. L. & Mekalanos, J. J. (1988). A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170, 2575-2583.
34. Montgomerie, J. Z. (1979). Epidemiology of Klebsiella and hospital-associated infections. Rev Infect Dis 1, 736-753.
35. Nikado, H. (1996). Outer membrane. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn, vol. I, pp. 29-47. Edited by F. C. Niedhardt and others. Washington, DC: American Society for Microbiology.
36. Oelschlaeger, T. A. & Tall, B. D. (1997). Invasion of cultured human epithelial cells by Klebsiella pneumoniae isolated from the urinary tract. Infect Immun 65, 2950-2958.
37. Ørskov, I. & Ørskov, F. (1984). Serotyping of Klebsiella. In Methods in Microbiology, pp. 143-164. Edited by T. Bergan. London: Academic Press.
38. Podschun, R. & Ullmann, U. (1998). Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev 11, 589-603.
39. Prasadarao, N. V., Wass, C. A., Weiser, J. N., Stins, M. F., Huang, S. H. & Kim, K. S. (1996). Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells. Infect Immun 64, 146-153.
40. Regue, M., Climent, N., Abitiu, N., Coderch, N., Merino, S., Izquierdo, L., Altarriba, M. & Tomas, J. M. (2001). Genetic characterization of the Klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis. J Bacteriol 183, 3564-3573.
41. Shannon, K., Stapleton, P., Xiang, X., Johnson, A., Beattie, H., El Bakri, F., Cookson, B. & French, G. (1998). Extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains causing nosocomial outbreaks of infection in the United Kingdom. J Clin Microbiol 36, 3105-3110.
42. Shea, J. E., Santangelo, J. D. & Feldman, R. G. (2000). Signature-tagged mutagenesis in the identification of virulence genes in pathogens. Curr Opin Microbiol 3, 451-458.
43. Sokurenko, E. V., Chesnokova, V., Dykhuizen, D. E., Ofek, I., Wu, X. R., Krogfelt, K. A., Struve, C., Schembri, M. A. & Hasty, D. A. (1998). Pathogenic adaptation of Escherichia coli by natural variation of the FimH adhesin. Proc Natl Acad Sci U S A 95, 8922-8926.
44. Struve, C. & Krogfelt, K. A. (1999). In vivo detection of Escherichia coli type 1 fimbrial expression and phase variation during experimental urinary tract infection. Microbiology 145, 2683-2690.
45. Sydenham, M., Douce, G., Bowe, F., Ahmed, S., Chatfield, S. & Dougan, G. (2000). Salmonella enterica serovar Typhimurium surA mutants are attenuated and effective live oral vaccines. Infect Immun 68, 1109-1115.
46. Tormo, A., Almiron, M. & Kolter, R. (1990). surA, an Escherichia coli gene essential for survival in stationary phase. J Bacteriol 172, 4339-4347.
47. Tsai, C. M. & Frasch, C. E. (1982). A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119, 115-119.
48. Tsolis, R. M., Townsend, S. M., Miao, E. A., Miller, S. I., Flicht, T. A., Adams, L. G. & Bäumler, A. J. (1999). Identification of a putative Salmonella enterica serotype Typhimurium host range factor with homology to IpaH and YopM by signature-tagged mutagenesis. Infect Immun 67, 6385-6393.
49. Verma, A., Desai, N., Shannon, K., Philpott-Howard, J. & Hill, R. L. (2001). Intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other enterobacteria. Int J Antimicrob Agents 17, 123-129.
50. Wada, M., Kano, Y., Ogawa, T., Okazaki, T. & Imamoto, F. (1988). Construction and characterization of the deletion mutant of hupA and hupB genes in Escherichia coli. J Mol Biol 204, 581-591.
51. Weiser, J. N. & Gotschlich, E. C. (1991). Outer membrane protein A (OmpA) contributes to serum resistance and pathogenicity of Escherichia coli K-1. Infect Immun 59, 2252-2258,
52. Zhang, Y. & Cronan, J. E. J. (1998). Transcriptional analysis of essential genes of the Escherichia coli fatty acid biosynthesis gene cluster by functional replacement with the analogous Salmonella typhimurium gene cluster. J Bacteriol 180, 3295-3303.
53. Zhao, H., Li, X., Johnson, D. E. & Mobley, H. L. T. (1999). Identification of protease and rpoN-associated genes of uropathogenic Proteus mirabilis by negative selection in a mouse model of ascending urinary tract infection. Microbiology 145, 185-195.
54. Zuurmond, A. M., Rundlof, A. K. & Kraal, B. (1999). Either of the chromosomal tuf genes of E. coli K-12 can be deleted without loss of cell viability. Mol Gen Genet 260, 603-607.