Identification of protease and rpoN-associated genes of uropathogenic Proteus mirabilis by negative selection in a mouse model of ascending urinary tract infection

Microbiology

Volumn 145, Issue 1, 1999, Pages 185-195

  Zhao, Hui ^a   Li, Xin ^a   Johnson, David E ^a   Mobley, Harry L T ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

CBA mouse; Proteus mirabilis; Signature tagged mutagenesis (STM); Urinary tract infection

Indexed keywords

BACTERIAL ENZYME; KANAMYCIN; PROTEINASE; TETRACYCLINE;

ANIMAL MODEL; ARTICLE; AUXOTROPHIC MUTANT; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; DOT HYBRIDIZATION; GENE SEQUENCE; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OPERON; PHENOTYPE; PRIORITY JOURNAL; PROTEUS MIRABILIS; SOUTHERN BLOTTING; TRANSPOSON; URINARY TRACT INFECTION;

ANIMALIA; BACTERIA (MICROORGANISMS); LURIA; MIRABILIS; NEGIBACTERIA; POSIBACTERIA; PROTEUS MIRABILIS;

EID: 0032897759     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-1-185     Document Type: Article

References (60)

1. Allison, C. & Hughes, C. (1991). Closely linked genetic loci required for swarm cell differentiation and multicellular migration by Proteus mirabilis. Mol Microbiol 5, 1975-1982.
2. Allison, C., Emody, L., Coleman, N. & Hughes, C. (1994). The role of swarm cell differentiation and multicellular migration in the uropathogenicity of Proteus mirabilis. J Infect Dis 169, 1155-1158.
3. Bahrani, F. K. & Mobley, H. L. T. (1994). Proteus mirabilis MR/P fimbrial operon: genetic organization, nucleotide sequence, and conditions for expression. J Bacteriol 176, 3412-3419.
4. Bahrani, F. K., Massad, G., Lockatell, C. V., Johnson, D. E., Russell, R., Warren, J. W. & Mobley, H. L. T. (1994). Construction of a MR/P fimbrial mutant of Proteus mirabilis : role in virulence in a mouse model of ascending urinary tract infection. Infect Immun 62, 3363-3371.
5. Belas, R. & Flaherty, D. (1994). Sequence and genetic analysis of multiple flagellin-encoding genes from Proteus mirabilis. Gene 128, 33-41.
6. Belas, R., Erskine, D. & Flaherty, D. (1991a). Transposon mutagenesis in Proteus mirabilis. J Bacteriol 173, 6289-6293.
7. Belas, R., Erskine, D. & Flaherty, D. (1991b). Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behavior. J Bacteriol 173, 6279-6288.
8. Bender, R. A., Janssen, K. A., Resnick, A. D., Blumenberg, M., Foor, F. & Magasanik, B. (1977). Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. J Bacteriol 129, 1001-1009.
9. Brunder, W., Schmidt, H. & Karch, H. (1997). EspP, a novel extracellular serine protease of enterohaemorrhagic E. coli O157 : H7 cleaves human coagulation factor V. Mol Microbiol 24, 767-778.
10. Chiang, S. L. & Mekalanos, J. J. (1998). Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol Microbiol 27, 797-805.
11. Chowdhury, M. A., Miyoshi, S. & Shinoda, S. (1991). Vascular permeability enhancement by Vibrio mimicus protease and the mechanisms of action. Microbiol Immunol 35, 1049-1058.
12. Finkelstein, R. A., Boesman-Finkelstein, M., Chang, Y. & Hase, C. C. (1992). Vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment. Infect Immun 60, 472-478.
13. Griffith, D. P., Musher, D. M. & Itin, C. (1976). Urease : the primary cause of infection-induced urinary stones. Investig Urol 13, 346-350.
14. Hagberg, L., Engberg, I., Freter, R., Lam, J., Olling, S. & Svanborg-Eden, C. (1983). Ascending unobstructed urinary tract infection in mice caused by pyelonephritogenic Escherichia coli of human origin. Infect Immun 40, 273-283.
15. Hamilton-Miller, J. M. T. & Gargan, R. A. (1977). Rapid screening for urease inhibitors. Investig Urol 16, 327-328.
16. Häse, C. C. & Finkelstein, R. A. (1993). Bacterial extracellular zinc-containing metalloproteases. Microbiol Rev 57, 823-837.
17. Hensel, M., Shea, J. E., Gleeson, C., Jones, M. D., Dalton, E. & Holden, D. W. (1995). Simultaneous identification of bacterial virulence genes by negative selection. Science 269, 400-403.
18. Hu, L.-T., Nicholson, E. B., Jones, B. D., Lynch, M. J. & Mobley, H. L. T. (1990). Morganella morganii urease: purification, characterization, and isolation of gene sequences. J Bacteriol 172, 3073-3080.
19. Imaishi, H., Gomada, M., Inouye, S. & Nakazawa, A. (1993). Physical map location of the rpoN gene of Escherichia coli. J Bacteriol 175, 1550-1551.
20. Johnson, D. E., Lockatell, C. V., Hall-Craigs, M., Mobley, H. L. T. & Warren, J. W. (1987). Uropathogenicity in rats and mice of Providencia stuartii from long-term catheterized patients. J Urol 138, 632-635.
21. Johnson, D. E., Russell, R. G., Lockatell, C. V., Zulty, J. C. & Warren, J. W. (1993a). Urethral obstruction of 6 hours or less causes bacteriuria, bacteremia, and pyelonephritis in mice challenged with 'nonuropathogenic' Escherichia coli. Infect Immun 61, 3422-3428.
22. Johnson, D. E., Russell, R. G., Lockatell, C. V., Zulty, J. C., Warren, J. W. & Mobley, H. L. T. (1993b). Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection. Infect Immun 61, 2748-2754.
23. Jones, B. D. & Mobley, H. L. T. (1987). Genetic and biochemical diversity of ureases of Proteus, Providencia, and Morganella species isolated from urinary tract infection. Infect Immun 55, 2198-2202.
24. Jones, B. D. & Mobley, H. L. T. (1988). Proteus mirabilis urease : genetic organization, regulation, and expression of structural genes. J Bacteriol 170, 3342-3349.
25. Jones, B. D., Lockatell, C. V., Johnson, D. E., Warren, J. W. & Mobley, H. L. T. (1990). Construction of a urease-negative mutant of Proteus mirabilis : analysis of virulence in a mouse model of ascending urinary tract infection. Infect Immun 58, 1120-1123.
26. 54 of Escherichia coli. Microbiology 140, 1035-1043.
27. Kato, T., Takahashi, N. & Kuramitsu, H. K. (1992). Sequence analysis and characterization of the Porphyromonas gingivalis prtC gene, which expresses a novel collagenase activity. J Bacteriol 174, 3889-3895.
28. Kontani, M., Kimura, S., Nakagawa, I. & Hamada, S. (1997). Adherence of Porphyromonas gingivalis to matrix proteins via a fimbrial cryptic receptor exposed by its own arginine-specific protease. Mol Microbiol 24, 1179-1187.
29. Loomes, L. M., Senior, B. W. & Kerr, M. A. (1990). A proteolytic enzyme secreted by Proteus mirabilis degrades immunoglobulins of the immunoglobulin A1(IgA1), IgA2, and IgG isotypes. Infect Immun 58, 1979-1985.
30. Lorenzo, V., Herrero, M., Jakubzik, U. & Timmis, K. N. (1990). Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J Bacteriol 172, 6568-6572.
31. Mahan, M. J., Slauch, J. M. & Mekalanos, J. J. (1993). Selection of bacterial virulence genes that are specifically induced in host tissues. Science 259, 686-688.
32. Massad, G., Lockatell, C. V., Johnson, D. E. & Mobley, D. E. (1994). Proteus mirabilis fimbriae: construction of an isogenic pmfA mutant and analyses of virulence in a CBA mouse model of ascending urinary tract infection. Infect Immun 62, 536-542.
33. Massad, G., Zhao, H. & Mobley, H. L. T. (1995). Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad. J Bacteriol 177, 5878-5883.
34. Mei, J. M., Nourbakhsh, F., Ford, C. W. & Holden, D. W. (1997). Identification of Staphylococcus aureus virulence genes in a murine model of bacteraemia using signature-tagged mutagenesis. Mol Microbiol 26, 399-407.
35. N). Mol Microbiol 10, 903-909.
36. 54-dependent promoters. Mol Microbiol 3, 1765-1775.
37. Merrick, M. J. & Edwards, R. A. (1995). Nitrogen control in bacteria. Microbiol Rev 59, 604-622.
38. Mobley, H. L. T. (1996). Virulence of Proteus mirabilis. In Urinary Tract Infection: Molecular Pathogenesis and Clinical Management, pp. 245-271. Edited by H. L. T. Mobley & J. W. Warren. Washington, DC: American Society for Microbiology.
39. Mobley, H. L. T. & Belas, R. (1995). Swarming and pathogenicity of Proteus mirabilis in the urinary tract. Trends Microbiol 3, 280-284.
40. Mobley, H. L. T. & Warren, J. W. (1987). Urease-positive bacteriuria and obstruction of long-term urinary catheters. J Clin Microbiol 25, 2216-2217.
41. Mobley, H. L. T., Chippendale, G. R., Swihart, K. G. & Welch, R. (1991). Cytotoxicity of the HpmA hemolysin and urease of Proteus mirabilis and Proteus vulgaris against cultured human renal proximal tubular epithelial cells. Infect Immun 59, 2036-2042.
42. Mobley, H. L. T., Belas, R., Lockatell, C. V., Chippendale, G., Trifillis, A., Johnson, D. E. & Warren, J. W. (1996). Construction of a flagellum-negative mutant of Proteus mirabilis: effect and internalization by human renal epithelial cells and virulence in a mouse model of ascending urinary tract infection. Infect Immun 64, 5332-5340.
43. Nicholson, E. B., Concaugh, E. A., Foxall, P. A., Island, M. D. & Mobley, H. L. T. (1993). Proteus mirabilis urease: transcriptional regulation by ureR. J Bacteriol 175, 465-473.
44. Plaut, A. G. (1983). The IgA1 proteases of pathogenic bacteria. Annu Rev Microbiol 37, 603-622.
45. Plaut, A. G., Gilbert, J. V., Artenstein, M. S. & Capra, J. D. (1975). Neisseria gonorrhoeae and Neisseria meningitidis: extracellular enzyme cleaves human immunoglobulin A. Science 190, 1103-1105.
46. Powell, B. S., Court, D. L., Inada, T., Nakamura, Y., Michotey, V., Cui, X., Reizer, A., Saier, M. H., Jr & Reizer, J. (1995). Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. J Biol Chem 270, 4822-4839.
47. Reizer, J., Reizer, A., Saier, M. H., Jr & Jacobson, G. R. (1992). A proposed link between nitrogen and carbon metabolism involving protein phosphorylation in bacteria. Protein Sci 1, 722-726.
48. Rubin, R., Tolkoff-Rubin, N. & Cotran, R. (1986). Urinary tract infection, pyelonephritis and reflux nephropathy. In The Kidney, 3rd edn, pp. 1085-1141. Edited by G. Brenner & F. Rector, Jr. Philadelphia: W. B. Saunders.
49. Saier, M. H., Jr & Reizer, J. (1994). The bacterial phosphotransferase system: new frontiers 30 years later. Mol Microbiol 13, 755-764.
50. Schwan, W. R., Silvija, N. C., Ng, E. Y. W. & 7 other authors (1998). Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models. Infect Immun 66, 567-572.
51. Senior, B. W., Albrechtsen, M. & Kerr, M. A. (1987). Proteus mirabilis strains of diverse type have IgA protease activity. J Med Microbiol 24, 175-180.
52. Senior, B. W., Loomes, L. M. & Kerr, M. A. (1991). The production and activity in vivo of Proteus mirabilis IgA protease in infections of the urinary tract. J Med Microbiol 35, 203-207.
53. Setia, U., Serventi, I. & Lorenz, P. (1984). Bacteremia in a longterm care facility: spectrum and mortality. Arch Intern Med 144, 1633-1635.
54. Shah, H. N., Seddon, S. U. & Gharbia, S. E. (1989). Studies on the virulence properties and metabolism of pleiotropic mutants of Porphyromonas gingivalis W50. Oral Microbiol Immunol 4, 19-23.
55. Shea, J. E., Hensel, M., Gleeson, C. & Holden, D. W. (1996). Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium. Proc Natl Acad Sci USA 93, 2593-2597.
56. Swihart, K. G. & Welch, R. A. (1990). Cytotoxic activity of the Proteus hemolysin HpmA. Infect Immun 58, 1861-1867.
57. Warren, J. W., Tenney, J. H., Hoopes, J. M., Muncie, H. L. & Anthony, W. C. (1982). A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters. J Infect Dis 146, 719-723.
58. Wassif, C., Cheek, D. & Belas, R. (1995). Molecular analysis of a metalloprotease from P. mirabilis. J Bacteriol 177, 5790-5798.
59. Wright, D. K. & Manos, M. M. (1990). Sample preparation from paraffin-embedded tissues. In PCR Protocols: a Guide to Methods and Applications, pp. 1153-H56. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White. New York : Academic Press.
60. Zhao, H., Li, X., Johnson, D. E., Blomfield, I. & Mobley, H. L T. (1997). In vivo phase variation of MR/P fimbrial gene expression in Proteus mirabilis infecting the urinary tract. Mol Microbiol 23, 1009-1019.