A mutational analysis of the active site of human type II inosine 5′-monophosphate dehydrogenase

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1594, Issue 1, 2002, Pages 27-39

  Futer, Olga ^a   Sintchak, Michael D ^a   Caron, Paul R ^a   Nimmesgern, Elmar ^a   DeCenzo, Maureen T ^a   Livingston, David J ^a   Raybuck, Scott A ^a  

^a VERTEX PHARMACEUTICALS   (United States)

Author keywords

Active site; Inosine monophosphate dehydrogenase; Mechanism of catalysis; Mycophenolic acid; Site directed mutagenesis; X ray structure

Indexed keywords

ARGININE; ASPARAGINE; ASPARTIC ACID; CYSTEINE; GLUTAMINE; INOSINATE DEHYDROGENASE; MYCOPHENOLIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; RIBOSE; THREONINE; XANTHENE;

ARTICLE; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; HUMAN; HYDROGEN BOND; HYDROLYSIS; MUTATION; MUTATIONAL ANALYSIS; OXIDATION; PRIORITY JOURNAL;

BINDING SITES; CATALYSIS; HYDROLYSIS; IMP DEHYDROGENASE; INOSINE MONOPHOSPHATE; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; NAD; RIBONUCLEOTIDES;

EID: 0037203857     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(01)00277-1     Document Type: Article

References (36)

1. Tissue-differential expression of two distinct genes for human IMP dehydrogenase (E.C.1.1.1.205)
2. Inhibition of IMPDH by mycophenolic acid: Dissection of forward and reverse pathways using capillary electrophoresis
3. Two distinct cDNAs for human IMP dehydrogenase
4. Selective up-regulation of type II inosine 5′-monophosphate dehydrogenase messenger RNA expression in human leukemias
5. Expression of human IMP dehydrogenase Types I and II in Escherichia coli and distribution in human normal lymphocytes and leukemic cell lines
6. Proliferation-linked regulation of type II IMP dehydrogenase gene in human normal lymphocytes and HL-60 leukemic cells
7. Increased inosine-5′-phosphate dehydrogenase gene expression in solid tumor tissues and tumor cell lines
8. Effects of human T lymphocyte activation on inosine monophosphate dehydrogenase expression
9. Immunosuppressive and other effects of mycophenolic acid and an ester prodrug, mycophenolate mofetil
10. Probing the active site of human IMP dehydrogenase using halogenated purine riboside 5′-monophosphate and covalent modification reagents
11. Identification of the IMP binding site in the IMP dehydrogenase from Tritrichomonas foetus
12. Inactivation of inosine 5′-monophosphate dehydrogenase by the antiviral agent 5-ethynyl-1-β-D-ribofuranosylimidazole-4-carboxamide 5′-monophosphate
13. Formation and rate-determining hydrolysis of a covalent intermediate by human type II IMP dehydrogenase
14. Trapping of an IMP dehydrogenase-substrate covalent intermediate by mycophenolic acid
15. Structure and mechanism of inosine monophosphate dehydrogenase in complex with the immunosuppressant mycophenolic acid
16. Biochemical analysis of the modular enzyme inosine 5′-monophosphate dehydrogenase
17. A novel method for site-directed mutagenesis using PCR and uracil DNA glycosylase
18. Genetic and biochemical studies of the guanosine 5′-monophosphate pathway in Escherichia coli
19. Inosine 5′-phosphate dehydrogenase. Kinetic mechanism and evidence for selective reaction of the 6-chloro analog of inosine 5′-phosphate with a cysteine residue at the inosine 5′-phosphate site
20. Purification, characterization, and kinetic analysis of inosine 5′-monophosphate dehydrogenase of Tritrichomonas foetus
21. The structure of inosine 5′-monophosphate dehydrogenase and the design of novel inhibitors
22. Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions
23. The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog
24. Monovalent cation activation in Escherichia coli inosine 5′-monophosphate dehydrogenase
25. Crystal structure of Tritrichomonas foetus inosine-5′-monophosphate dehydrogenase and the enzyme-product complex
26. Tritrichomonas foetus: A strategy for structure-based inhibitor design of a protozoan inosine-5′-monophosphate dehydrogenase
27. IMP dehydrogenase: Structural aspects of inhibitor binding
28. Crystal structure of human type II inosine monophosphate dehydrogenase: Implications for ligand binding and drug design
29. The conformation of NADH bound to inosine 5′-monophosphate dehydrogenase determined by transferred nuclear Overhauser effect spectroscopy
30. Inhibitors of inosinic acid dehydrogenase. 2-Substituted inosinic acids
31. Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 Å resolution
32. Kinetic mechanism and cofactor content of soybean root nodule urate oxidase
33. Identification of the true product of the urate oxidase reaction
34. Species-specific inhibition of inosine 5′-monophosphate dehydrogenase by mycophenolic acid
35. LIGPLOT: A program to generate schematic diagrams of protein-ligand interactions