Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR

Applied and Environmental Microbiology

Volumn 67, Issue 2, 2001, Pages 972-976

  Hermansson, A ^a   Lindgren, P E ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; FERTILIZER; NITROGEN; PRIMER DNA; RIBOSOME DNA;

MICROBIOLOGY;

ARTICLE; BACTERIAL COUNT; BACTERIUM ADHERENCE; BACTERIUM IDENTIFICATION; DNA PROBE; DNA TEMPLATE; GRAM NEGATIVE BACTERIUM; LYSIS; NONHUMAN; OXIDATION; POLYMERASE CHAIN REACTION; SOIL MICROFLORA; SOIL TREATMENT; SWEDEN;

AMMONIA; DNA, RIBOSOMAL; OXIDATION-REDUCTION; POLYMERASE CHAIN REACTION; PROTEOBACTERIA; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS); BETAPROTEOBACTERIA; NEGIBACTERIA; NITROSOMONADALES; PROTEOBACTERIA;

EID: 0035144174     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.2.972-976.2001     Document Type: Article

References (32)

1. Surface attachment of ammonia-oxidising bacteria in soil
2. RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria: A phylogenetic approach
3. A versatile ELISA-PCR assay for mRNA quantitation from a few cells
4. Recovery of bacterial cells from soil
5. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
6. Comparative diversity of ammonia oxidizer 16S rRNA gene sequences in native, tilled, and successional soils
7. + oxidisers in a coniferous forest soil
8. A dilute medium to determine population size of ammonium oxidizers in soil
9. Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species
10. Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis finger-prints
11. Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction
12. On the evolutionary descent of organisms and organelles: A global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA
13. Real time quantitative PCR
14. Simultaneous amplification and detection of specific DNA sequences
15. Multiple copies of ammonia mono-oxygenase (amo) operons have evolved under biased AT/GC mutational pressure in ammonia-oxidizing autotrophic bacteria
16. Molecular analysis of ammonia-oxidizing bacteria of the β subdivision of the class Proteobacteria in compost and composted materials
17. Analysis of ammonia-oxidizing bacteria of the β subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments
18. Molecular analysis of ammonia-oxidising bacteria in soil of successional grasslands of the Drentsche A (The Netherlands)
19. Effects of organic manure on nitrification in arable soils
20. A selective and diagnostic medium for ammonia oxidising bacteria
21. Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea
22. Use of molecular and isotopic techniques to monitor the response of autotrophic ammonia-oxidizing populations of the β subdivision of the class Proteobacteria in arable soils to nitrogen fertilizer
23. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis
24. The gene encoding ammonia monooxygenase subunit A exists in three nearly identical copies in Nitrosospira sp
25. Quantitative analysis of ammonia oxidising bacteria using competitive PCR
26. Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences
27. Quantification and characterisation of Nitrosomonas and Nitrobacter using monoclonal antibodies
28. Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: Quantification by in situ hybridization and the use of microsensors
29. Effect of toxic metals on indigenous soil β-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria
30. Quantitation of targets for PCR by use of limiting dilution