Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR

Applied and Environmental Microbiology

Volumn 67, Issue 2, 2001, Pages 760-768

  Gruntzig V ^a   Nold, S C ^a   Zhou, J ^a   Tiedje, J M ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GROUND WATER;

MICROBIOLOGY;

ARTICLE; BACTERIAL GENE; BIODEGRADATION; DENITRIFICATION; GENE ISOLATION; GENETIC VARIABILITY; LAKE; NONHUMAN; POLYMERASE CHAIN REACTION; PSEUDOMONAS STUTZERI; SEA; SEDIMENT;

DNA, BACTERIAL; ENVIRONMENTAL MICROBIOLOGY; NITRITE REDUCTASES; POLYMERASE CHAIN REACTION; PSEUDOMONAS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 0035146327     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.2.760-768.2001     Document Type: Article

References (45)

1. The effect of 2,4-dichlorophenoxyacetate selection on microbial communities in microcosm and field studies and the impact on ecosystem function
2. Quantitation of MYC gene expression in sporadic breast tumors with a real-time reverse transcription-PCR assay
3. Novel approach to quantitative polymerase chain reaction using real-time detection: Application to the detection of gene amplification in breast cancer
4. The complete genome sequence of Escherichia coli K-12
5. Fluorescent staining of microbes for total direct counts
6. Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples
7. Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in Pacific Northwest marine sediment communities
8. 1 and non-heme copper nitrite reductase in denitrifying bacteria
9. Transformation of carbon tetrachloride by Pseudomonas sp. strain KC under denitrification conditions
10. Quantitative detection of t(14; 18)-positive cells by real-time quantitative PCR using fluorogenic probes
11. Biological denitrification
12. Numerically dominant denitrifying bacteria from world soils
13. A novel method for real time quantitative RT-PCR
14. Genome organization of Pseudomonas stutzeri and resulting taxonomic and evolutionary considerations
15. Phylogenetic analysis of the bacterial communities in marine sediments
16. One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses
17. PCR detection of genes encoding nitrite reductase in denitrifying bacteria
18. Quantitative measurement of Stachybotrys chartarum conidia using real time detection of PCR products with the TaqMan™ fluorogenic probe system
19. Real time quantitative PCR
20. Detection of specific polymerase chain reaction product by utilizing the 5′-3′exonuclease activity of Thermus aquaticus DNA polymerase
21. Hydraulic characterization and design of a full-scale biocurtain
22. A survey of 16S rRNA and amoA genes related to autotrophic ammonia-oxidizing bacteria of the β-subdivision of the class proteobacteria in contaminated groundwater
23. Detection and quantitation of human papillomavirus by using the fluorescent 5′ exonuclease assay
24. 1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins
25. Differential quantitation of alternatively spliced messenger RNAs using isoform-specific real-time RT-PCR
26. A species-specific probe and a PCR assay for the marine bacterium, Pseudomonas stutzeri strain Zobell
27. Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay
28. Denitrification
29. 1) among denitrifying pseudomonads
30. Allelic discrimination by nick-translation PCR with fluorogenic probes
31. Novel 5′ exonuclease-based real-time PCR assay for the detection of t(14; 18)(q32; q21) in patients with follicular lymphoma
32. High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA
33. 1-denitrifying bacteria in environmental marine samples
34. A combined physical and genetic map of Pseudomonas aeruginosa PAO
35. Diversity of nitrous oxide reductase (nosZ) genes in continental shelf sediments
36. Denitrification in aquatic sediments
37. Isolation and characterization of a nitrite reductase gene and its use as a probe for denitrifying bacteria
38. Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays
39. Ecology of denitrification and dissimilatory nitrate reduction to ammonium
40. Extraction of microbial community DNA from soils
41. mRNA quantification by real time Taq-Man polymerase chain reaction: Validation and comparison with RNase protection
42. Immunofluorescence detection of the denitrifying strain Pseudomonas stutzeri (ATCC 14405) in seawater and intertidal sediment environments
43. Cell biology and molecular basis of denitrification