Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF59-U2AF23

EMBO Journal

Volumn 21, Issue 20, 2002, Pages 5516-5526

  Huang, Tao ^a   Vilardell, Josep ^b   Query, Charles C ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b CENTRE FOR GENOMIC REGULATION CRG   (Spain)

Author keywords

S.pombe; SF1 BBP; U2 5 6 tri snRNP; U2AF U2 SnRNP

Indexed keywords

ADENOSINE TRIPHOSPHATE; CELL PROTEIN; HYBRID PROTEIN; PROTEIN SF1; PROTEIN SUBUNIT; PROTEIN U2AF; PYRIMIDINE; SMALL NUCLEAR RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; DISSOCIATION; INTRON; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STABILITY; RNA SEQUENCE; RNA SPLICING; SCHIZOSACCHAROMYCES POMBE; SPLICEOSOME;

ADENOSINE TRIPHOSPHATE; BASE SEQUENCE; MACROMOLECULAR SUBSTANCES; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN SUBUNITS; RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA SPLICING; RNA, FUNGAL; RNA-BINDING PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SPLICEOSOMES;

MAMMALIA; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 0037107372     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf555     Document Type: Article

References (39)

1. Abovich, N. and Rosbash, M. (1997) Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell, 89, 403-412.
2. Ansari, A. and Schwer, B. (1995) SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. EMBO J., 14, 4001-4009.
3. Arenas, J.E. and Abelson, J.N. (1997) Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc. Natl. Acad. Sci. USA, 94, 11798-11802.
4. Bahler, J., Wu, J.Q., Longtine, M.S., Shah, N.G., McKenzie, A., III, Steever, A.B., Wach, A., Philippsen, P. and Pringle, J.R. (1998) Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast, 14, 943-951.
5. Beales, M., Flay, N., McKinney, R., Habara, Y., Ohshima, Y., Tani, T. and Potashkin, J. (2000) Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure. Yeast, 16, 1001-1013.
6. Berglund, J.A., Chua, K., Abovich, N., Reed, R. and Rosbash, M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell, 89, 781-787.
7. Berglund, J.A., Abovich, N. and Rosbash, M. (1998) A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. Genes Dev., 12, 858-867.
8. Brennwald, P., Porter, G. and Wise, J.A. (1988) U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals. Mol. Cell. Biol., 8, 5575-5580.
9. Burge, C.B., Tuschl, T.H. and Sharp, P.A. (1999) Splicing of precursors to mRNAs by the spliceosomes. In Gesteland, R.F., Cech, T.R. and Atkins, J.F. (eds), The RNA World, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 525-560.
10. Chabot, B., Black, D.L., LeMaster, D.M. and Steitz, J.A. (1985) The 3′ splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein. Science, 230, 1344-1349.
11. Das, R., Zhou, Z. and Reed, R. (2000) Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol. Cell, 5, 779-787.
12. Grabowski, P.J., Padgett, R.A. and Sharp, P.A. (1984) Messenger RNA splicing in vitro: An excised intervening sequence and a potential intermediate. Cell, 37, 415-427.
13. Guth, S. and Valcárcel, J. (2000) Kinetic role for mammalian SF1/BBP in spliceosome assembly and function after polypyrimidine tract recognition by U2AF. J. Biol. Chem., 275, 38059-38066.
14. Jurica, M.S., Licklider, L.J., Gygi, S.R., Grigorieff, N. and Moore, M.J. (2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA, 8, 426-439.
15. Käufer, N.F. and Potashkin, J. (2000) Analysis of the splicing machinery in fission yeast: A comparison with budding yeast and mammals. Nucleic Acids Res., 28, 3003-3010.
16. Konarska, M.M. and Sharp, P.A. (1987) Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell, 49, 763-774.
17. Krämer, A. (1992) Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex. Mol. Cell. Biol., 12, 4545-4552.
18. Krämer, A. and Utans, U. (1991) Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs. EMBO J., 10, 1503-1509.
19. Liao, X.C., Colot, H.V., Wang, Y. and Rosbash, M. (1992) Requirements for U2 snRNP addition to yeast pre-mRNA. Nucleic Acids Res., 20, 4237-4245.
20. Martin, A., Schneider, S. and Schwer, B. (2002) Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome. J. Biol. Chem., 277, 17743-17750.
21. Mazroui, R., Puoti, A. and Krämer, A. (1999) Splicing factor SF1 from Drosophila and Caenorhabditis: Presence of an N-terminal RS domain and requirement for viability. RNA, 5, 1615-1631.
22. Merendino, L., Guth, S., Bilbao, D., Martinez, C. and Valcárcel, J. (1999) Inhibition of msl-2 splicing by Sex-lethal reveals interaction between U2AF35 and the 3′ splice site AG. Nature, 402, 838-841.
23. Moore, M.J., Query, C.C. and Sharp, P.A. (1993) Splicing of precursors to mRNA by the spliceosome. In Gesteland, R. and Atkins, J. (eds.), The RNA World. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 303-357.
24. Ohi, M.D., Link, A.J., Ren, L., Jennings, J.L., McDonald, W.H. and Gould, K.L. (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and building yeasts containing myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors and snRNAs. Mol. Cell. Biol., 22, 2011-2024.
25. Okazaki, K. and Niwa, O. (2000) mRNAs encoding zinc finger protein isoforms are expressed by alternative splicing of an in-frame intron in fission yeast. DNA Res., 7, 27-30.
26. Potashkin, J., Li, R. and Frendewey, D. (1989) Pre-mRNA splicing mutants of Schizosaccharomyces pombe. EMBO J., 8, 551-559.
27. Potashkin, J., Naik, K. and Wentz-Hunter, K. (1993) U2AF homolog required for splicing in vivo. Science, 262, 573-575.
28. Puig, O., Caspary, F., Rigaut, G., Rutz, B., Bouveret, E., Bragado-Nilsson, E., Wilm, M. and Séraphin, B. (2001) The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods, 24, 218-229.
29. Rain, J.C., Rafi, Z., Rhani, Z., Legrain, P. and Krämer, A. (1998) Conservation of functional domains involved in RNA binding and protein-protein interactions in human and Saccharomyces cerevisiae pre-mRNA splicing factor SF1. RNA, 4, 551-565.
30. Roinfo, C.M. and Wise, J.A. (1997) Both the polypyrimidine tract and the 3′ splice site function prior to the first step of splicing in fission yeast. Nucleic Acids Res., 25, 4658-4665.
31. Rutz, B. and Séraphin, B. (1999) Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly. RNA, 5, 819-831.
32. Stevens, S.W., Ryan, D.E., Ge, H.Y., Moore, R.E., Young, M.K., Lee, T.D. and Abelson, J. (2002) Composition and functional characterization of the yeast spliceosomal penta-snRNP. Mol. Cell, 9, 31-44.
33. Umen, J.G. and Guthrie, C. (1995) The second catalytic step of pre-mRNA splicing. RNA, 1, 869-885.
34. Wang, X., Bruderer, S., Rafi, Z., Xue, J., Milburn, P.J., Krämer, A. and Robinson, P.J. (1999) Phosphorylation of splicing factor SFI on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly. EMBO J., 18, 4549-4559.
35. Wentz-Hunter, K. and Potashkin, J. (1996) The small subunit of the splicing factor U2AF is conserved in fission yeast. Nucleic Acids Res., 24, 1849-1854.
36. Will, C.L., Schneider, C., MacMillan, A.M., Katopodis, N.F., Neubauer, G., Wilm, M., Lührmann, R. and Query, C.C. (2001) A novel U2 and U11/U12 snRNP protein that associates with the pre-MRNA branch site. EMBO J., 20, 4536-4546.
37. Wood, V. et al. (2002) The genome sequence of Schizosaccharomyces pombe. Nature, 415, 871-880.
38. Wu, S., Romfo, C.M., Nilsen, T.W. and Green, M.R. (1999) Functional recognition of the 3′ splice site AG by the splicing factor U2AF35. Nature, 402, 832-835.
39. Zorio, D.A. and Blumenthal, T. (1999) Both subunits of U2AF recognize the 3′ splice site in Caenorhabditis elegans. Nature, 402, 835-838.