Expression of the human DNA glycosylase hSMUG1 in Trypanosoma brucei causes DNA damage and interferes with J biosynthesis

Nucleic Acids Research

Volumn 30, Issue 18, 2002, Pages 3919-3926

  Ulbert, Sebastian ^a   Cross, Mike ^a   Boorstein, Robert J ^b   Teebor, George W ^b   Borst, Piet ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

^b NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5 HYDROXYMETHYLDEOXYURIDINE; 5 HYDROXYMETHYLURACIL; BETA D GLUCOSYL HYDROXYMETHYLURACIL; CELL NUCLEUS DNA; ENZYME PRECURSOR; THYMINE; UNCLASSIFIED DRUG; URACIL DERIVATIVE; URACIL DNA GLYCOSYLTRANSFERASE;

ARTICLE; CELL CYCLE; CELL DEATH; CONTROLLED STUDY; DNA DAMAGE; DNA MODIFICATION; DNA SEQUENCE; ENZYME SYNTHESIS; EUKARYOTE; EXCISION REPAIR; GENE EXPRESSION; HUMAN; MITOSIS INHIBITION; NONHUMAN; NUCLEOTIDE REPEAT; PRIORITY JOURNAL; TRYPANOSOMA BRUCEI;

EUKARYOTA; KINETOPLASTIDA; MASTIGOPHORA (FLAGELLATES); TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0037106680     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkf533     Document Type: Article

References (31)

1. Gommers-Ampt,J., van Leeuwen,F., de Beer,A.L.J., Vliegenthart, J.F.G., Didzdaroglu,M., Kowalak,J.A. and Borst,P. (1993) Beta-D-glucosyl-hydroxymethyluracil: a novel modified base present in the DNA of the parasitic protozoan Trypanosoma brucei. Cell, 75, 1129-1136.
2. van Leeuwen,F., Taylor,M.C., Mondragon,A., Moreau,H., Gibson,W., Kieft,R. and Borst,P. (1998) Beta-D-glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres. Proc. Natl Acad. Sci. USA, 95, 2366-2371.
3. van Leeuwen,F., Kieft,R., Cross,M. and Borst,P. (2000) Tandemly repeated DNA is a target for the partial replacement of thymine by beta-D-glucosyl-hydroxymethyluracil in Trypanosoma J brucei. Mol. Biochem. Parasitol., 109, 133-145.
4. van Leeuwen,F., Wijsman,E.R., Kuyl-Yeheskiely,E., van der Marel,G.A., van Boom,J.H. and Borst,P. (1996) The telomeric GGGTTA repeats of Trypanosoma brucei contain the hypermodified base J in both strands. Nucleic Acids Res., 24, 2476-2482.
5. Cross,M., Kieft,R., Sabatini,R., Wilm,M., de Kort,M., van der Marel,G.A., van Boom,J., van Leuuwen,F. and Borst,P. (1999) The modified base J is the target for a novel DNA-binding protein in kinetoplastid protozoans. EMBO J., 18, 6573-6581.
6. Gommers-Ampt,J., Teixeira,A.J.R., van de Werken,G., van Djk,W. and Borst,P. (1993) The identification of hydroxymethyluracil in DNA of Trypanosoma brucei. Nucleic Acids Res., 21, 2039-2043.
7. van Leeuwen,F., Kieft,R., Cross,M. and Borst,P. (1998) Biosynthesis and function of the modified DNA base β-D-glucosyl-hydroxymethyluracil in Trypanosoma brucei. Mol. Cell. Biol., 18, 5643-5651.
8. Haushalter,K.A., Stukenberg,P.T., Kirschner,M.W.and Verdine,G.L. (1999) Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors. Curr. Biol., 9, 174-185.
9. Schärer,O.D. and Jiricny,J. (2001) Recent progress in the biology, chemistry and structural biology of DNA glycosylases. Bioessays, 23, 270-281.
10. Krokan,H.E., Nilsen,H., Skorpen,F., Otterlei,M. and Slupphaug,G. (2000) Base excision repair of DNA in mammalian cells. FEBS Lett., 476, 73-77.
11. Boorstein,R.J., Cummings,A., Marenstein,D.R., Chan,M.K., Ma,Y., Neubert,T.A., Brown,S.M. and Teebor,G.W. (2001) Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1. J. Biol. Chem., 276, 41991-41997.
12. Nilsen,H., Haushalter,K.A., Robins,P., Barnes,D.E., Verdine,G.L. and Lindahl,T. (2001) Excision of deaminated cytosine from the vertebrate genome: role of the SMUG1 uracil-DNA glycosylase. EMBO J., 20, 4278-4286.
13. Boorstein,R.J., Chiu,L.N. and Teebor,G.W. (1989) Phylogenetic evidence of a role for 5-hydroxymethyluracil-DNA glycosylase in the maintenance of 5-methylcytosine in DNA. Nucleic Acids Res., 17, 7653-7661.
14. Cross,G.A.M. (1975) Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology, 71, 393-417.
15. Hirumi,H. and Hirumi,K. (1989) Continuous cultivation of Trypanosoma brucei bloodstream forms in a medium containing a low concentration of serum protein without feeder layers. J. Parasitol., 75, 985-989.
16. Biebinger,S., Wirtz,E., Lorenz,P. and Clayton,C. (1997) Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei. Mol. Biochem. Parasitol., 85, 88-112.
17. Rudenko,G., Chaves,I., Dirks-Mulder,A. and Borst,P. (1998) Selection for activation of a new variant surface glycoprotein gene expression site in Trypanosoma brucei can result in deletion of the old one. Mol. Biochem. Parasitol., 95, 97-109.
18. Carruthers,V.B., van der Ploeg,L.H. and Cross,G.A. (1993) DNA-mediated transformation of bloodstream-form Trypanosoma brucei. Nucleic Acids Res., 21, 2537-2538.
19. Chaves,I., Zomerdijk,J., Dirks-Mulder,A., Dirks,R.W., Raap,A.K. and Borst,P. (1998) Subnuclear localization of the active variant surface glycoprotein gene expression site in Trypanosoma brucei. Proc. Natl Acad. Sci. USA, 95, 12328-12333.
20. Muñoz-Jordan,J.L. and Cross,G.A. (2001) Telomere shortening and cell cycle arrest in Trypanosoma brucei expressing human telomeric repeat factor TRF1. Mol. Biochem. Parasitol., 114, 169-181.
21. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Ulbert,S., Chaves,I. and Borst,P. (2001) Expression site activation in Trypanosoma brucei with three marked variant surface glycoprotein gene expression sites. Mol. Biochem. Parasitol., 120, 225-235.
23. van Leeuwen,F., Wijsman,E.R., Kieft,R., van der Marcel,G.A., van Boom,J.H. and Borst,P. (1997) Localization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei. Genes Dev., 11, 3232-3241.
24. Wirtz,E. and Clayton,C. (1995) Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science, 268, 1179-1183.
25. Wang,Z. and Mosbaugh,D.W. (1989) Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a binding protein specific for uracil-DNA glycosylase. J. Biol. Chem., 264, 1163-1171.
26. Matthews,K.R. and Gull,K. (1994) Cycles within cycles: the interplay between differentiation and cell division in Trypanosoma brucei. Parasitol. Today, 10, 473-476.
27. Mi,L.J., Chaung,W., Horowitz,R., Teebor,G.W. and Boorstein,R.J. (2001) Excessive base excision repair of 5-hydroxymethyluracil from DNA induces apoptosis in Chinese hamster V79 cells containing mutant p53. Carcinogenesis, 22, 179-186.
28. Glassner,B.J., Rasmussen,L.J., Najarian,M.T., Posnick,L.M. and Samson,L.D. (1998) Generation of a strong mutator phenotype in yeast by imbalanced base excision repair. Proc. Natl Acad. Sci. USA, 95, 9997-10002.
29. Frosina,G. (2000) Overexpression of enzymes that repair endogenous damage to DNA. Eur. J. Biochem. 267, 2135-2149.
30. Kavli,B., Slupphaug.G., Mol,C.D., Arvai,A.S., Petersen,S.B., Tainer,J.A. and Krokan,H.E. (1996) Excision of cytosine and thymine from DNA by mutants of human uracil-DNA glycosylase. EMBO J., 15, 3442-3447.
31. Cross,M., Kieft,R., Sabatini,R., Dirks-Mulder,A., Chaves,I. and Borst,P. (2002) J binding protein increases the level and retention of the unusual base J in trypanosome DNA. Mol. Microbiol., in press.