Excision of cytosine and thymine from DNA by mutants of human uracil-DNA glycosylase

EMBO Journal

Volumn 15, Issue 13, 1996, Pages 3442-3447

  Kavli, Bodil ^a   Slupphaug, Geir ^a   Mol, Clifford D ^b   Arvai, Andrew S ^b   Petersen, Steffen B ^c   Tainer, John A ^b   Krokan, Hans E ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c SINTEF   (Norway)

Author keywords

Cytosine DNA glycosylase; Human uracil DNA glycosylase; Mutator enzymes; Site directed mutagenesis; Thymine DNA glycosylase

Indexed keywords

CYTOSINE; DNA; MUTANT PROTEIN; RIFAMPICIN; THYMINE; URACIL DNA GLYCOSYLTRANSFERASE;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; FELIS CATUS;

EID: 0030035663     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00710.x     Document Type: Article

References (21)

1. Barnes, D.E., Lindahl, T. and Sedgwick, B. (1993) DNA repair. Curr. Opin. Cel Biol., 5, 424-433.
2. Bjelland, S., Birkeland, N.K., Benneche, T., Volden, G. and Seeberg, E. (1994) DNA glycosylase activities for thymine residues oxidized in the methyl group are functions of the AlkA enzyme in Escherichia coli. J. Biol. Chem., 269, 30489-30495.
3. Boiteux, S., O'Conner, T.R., Lederer, R. Gouyette, A. and Laval, J. (1990) Homogeneous Escherichia coli FPG protein. J. Biol. Chem., 265, 3916-3922.
4. Cannon, S.V., Cummings, A. and Teebor, G.W. (1988) 5-hydroxymethylcytosine-DNA glycosylase in mammalian tissue. Biochim. Biophys. Res. Commun., 151, 1173-1179.
5. Friedberg, E.C., Walker, G.C. and Siede, W. (1995) DNA Repair and Mutagenesis. ASM Press, Washington, DC.
6. Krokan, H. and Wittwer, C.U. (1981) Uracil-DNA glycosylase from HeLa cells: general properties, substrate specificity and effects of uracil analogs. Nucleic Acids Res., 9, 2599-2613.
7. Kunkel, T.A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl Acad. Sci. USA, 82, 488-492.
8. Lindahl, T. and Nyberg, B. (1974) Heat-induced deamination of cytosine residues in DNA. Biochemistry, 13, 3405-3410.
9. Mol, C.D., Arvai, A.S., Slupphaug, G., Kavli, B., Alseth, I., Krokan, H.E. and Tainer, J.A. (1995a) Crystal structure and mutational analysis of human uracil-DNA glycosylase: structural basis for specificity and catalysis. Cell, 80, 869-878.
10. Mol, C.D., Arvai, A.S., Sanderson, R.J., Slupphaug, G., Kavli, B., Krokan, H.E., Mosbaugh, D.W. and Tainer, J.A. (1995b) Crystal structure of human uracil-DNA glycosylase in complex with a protein inhibitor: protein mimicry of DNA. Cell, 82, 701-708.
11. 6-methylguanine-DNA methyltransferase and uracil-DNA glycosylase in human organs. Carcinogenesis, 4, 1565-1568.
12. Olsen, L.C., Aasland, R., Wittwer, C.U., Krokan, H.E. and Helland, D.E. (1989) Molecular cloning of human uracil-DNA glycosylase, a highly conserved DNA repair enzyme. EMBO J., 8, 3121-3125.
13. Olsen, L.C., Aasland, R., Krokan, H.E. and Helland, D.E. (1991) Human uracil-DNA glycosylase complements E.coli ung mutants. Nucleic Acids Res., 19, 4473-4478.
14. Nedderman, P. and Jiricny, J. (1993) The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells. J. Biol. Chem., 268, 21218-21114.
15. Nedderman, P. and Jiricny, J. (1994) Efficient removal of uracil from G:U mispairs by the mismatch-specific thymine DNA glycosylase from HeLa cells. Proc. Natl Acad. Sci. USA, 91, 1642-1646.
16. Roy, R., Brooks, C. and Mitra, S. (1994) Purification and biochemical characterization of recombinant N-methylpurine-DNA glycosylase of the mouse. Biochemistry, 33, 15131-15140.
17. Sassanfar, M. and Roberts, J.W. (1990) Nature of the SOS-inducing signal in Escherichia coli: the involvement in DNA replication. J. Mol. Biol., 212, 79-96.
18. Savva, R., McAuley-Hecht, K., Brown, T. and Pearl, L. (1995) The structural basis of specific base-excision repair by uracil-DNA glycosylase. Nature, 373, 487-493.
19. Slupphaug, G., Eftedal, I., Kavli, B., Bharati, S., Helle, N.M., Haug, T., Levine, D.W. and Krokan, H.E. (1995) Properties of a recombinant human uracil-DNA glycosylase from the UNG gene and evidence that UNG encodes the major uracil-DNA glycosylase. Biochemistry, 34, 128-138.
20. Tye, B.K., Nyman, P.O., Lehman, I.R., Hochhauser, S. and Weiss, B. (1977) Transient accumulation of Okazaki-fragments as a result of uracil incorporation into nascent DNA. Proc. Natl Acad. Sci. USA, 74, 154-157.
21. Wilkinson, G.N. (1961) Statistical estimations in enzyme kinetics. Biochem. J., 80, 324-332.