Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity

Journal of Neuroscience Research

Volumn 69, Issue 6, 2002, Pages 869-879

  Kanemura, Yonehiro ^a,c   Mori, Hideki ^a   Kobayashi, Satoshi ^a   Islam, Omedul ^a   Kodama, Eri ^a   Yamamoto, Atsuyo ^a   Nakanishi, Youko ^a   Arita, Norio ^b   Yamasaki, Mami ^a,c   Okano, Hideyuki ^a,d   Hara, Masayuki ^a,e   Miyake, Jun ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b HYOGO COLLEGE OF MEDICINE   (Japan)

^c OSAKA NATIONAL HOSPITAL   (Japan)

^d KEIO UNIVERSITY   (Japan)

^e Osaka Prefecture University   (Japan)

Author keywords

ATP; Cell viability assay; Neural stem progenitor cells; WST 8

Indexed keywords

TETRAZOLIUM;

ARTICLE; ASSAY; CELL COUNT; CELL DIFFERENTIATION; CELL GROWTH; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; DNA SYNTHESIS; HUMAN; HUMAN CELL; IN VITRO STUDY; MEASUREMENT; PRIORITY JOURNAL; REPRODUCIBILITY; STEM CELL;

ADENOSINE TRIPHOSPHATE; ANTIMETABOLITES; BROMODEOXYURIDINE; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SEPARATION; CELL SURVIVAL; CELLS, CULTURED; FETUS; HUMANS; INDICATORS AND REAGENTS; NEURONS; PROSENCEPHALON; STEM CELLS; TETRAZOLIUM SALTS;

EID: 0037106152     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/jnr.10377     Document Type: Article

References (42)

1. Better cells for brain repair
2. Cell replacement therapies for central nervous system disorders
3. ABC transporters as phenotypic markers and functional regulators of stem cells
4. Heparin, but not other proteoglycans, potentiates the mitogenic effects of FGF-2 on mesencephalic precursor cells
5. In vitro expansion of a multipotent population of human neural progenitor cells
6. Serial propagation of human fibroblast
7. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity
8. p27Kip1 alters the response of cells to mitogen and is part of a cell-intrinsic timer that arrests the cell cycle and initiates differentiation
9. Transplantation of embryonic dopamine neurons for severe Parkinson's disease
10. Mammalian neural stem cells
11. Telomerase and mammalian ageing: A critical appraisal
12. Epidermal and fibroblast growth factors behave as mitogenic regulators for a single multipotent stem cell-like population from the subventricular region of the adult mouse forebrain
13. Human cell and aging
14. MTT-cell proliferation assay
15. Characterization of neurosphere cell phenotypes by flow cytometry
16. Enhancement of MTT, a tetrazolium salt, exocytosis by amyloid beta-protein and chloroquine in cultured rat astrocytes
17. Neural stem cells in the adult human brain
18. Musashi1: An evolutionally conserved marker for CNS progenitor cells including neural stem cells
19. Musashil, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity
20. Oligodendrocyte precursor cells reprogrammed to become multipotential CNS stem cells
21. Proliferation of different cell types in the brain
22. CNS stem cells express a new class of intermediate filament protein
23. The MTT cell viability assay for cytotoxicity testing in multidrug-resistant human leukemic cells
24. Hematopoietic competence is a rare property of neural stem cells that may depend on genetic and epigenetic alterations
25. Human neural precursor cells express low levels of telomerase in vitro and show diminishing cell proliferation with extensive axonal outgrowth following transplantation
26. Progenitor cells from human brain after death
27. Comparison of MTT and ATP-based assays for the measurement of viable cell number
28. Lineage restriction of neuroepithelial precursor cells from fetal human spinal cord
29. A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes
30. Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell
31. Neural stem cells and regulation of cell number
32. Restricted growth potential of rat neural precursors as compared to mouse
33. New prospects for human stem-cell therapy in the nervous system
34. A new method for the rapid and long-term growth of human neural precursor cells
35. Clonal analysis of oligodendrocyte development in culture: Evidence for a developmental clock that counts cell divisions
36. Control of progenitor cell number by mitogen supply and demand
37. Isolation and intracerebral grafting of nontransformed multipotential embryonic human CNS stem cells
38. Isolation and cloning of multipotential stem cells from the embryonic human CNS and establishment of transplantable human neural stem cell lines by epigenetic stimulation
39. bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells
40. Is there a neural stem cell in the mammalian forebrain?
41. Cell cycle regulators in neural stem cells and postmitotic neurons
42. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype