Cysteine residues in the D-galactose-H+ symport protein of Escherichia coli: Effects of mutagenesis on transport, reaction with N-ethylmaleimide and antibiotic binding

Biochemical Journal

Volumn 353, Issue 3, 2001, Pages 709-717

  McDonald, T P ^a   Henderson, P J F ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Glucose transporter; Membrane transport; Sugar H+ symporter; Thiol group specific reagent

Indexed keywords

ANTIBIOTIC AGENT; CARRIER PROTEIN; CYSTEINE; CYTOCHALASIN B; FORSKOLIN; GALACTOSE; N ETHYLMALEIMIDE; SERINE; SUGAR;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; DRUG BINDING; ESCHERICHIA COLI; MEMBRANE TRANSPORT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CALCIUM-BINDING PROTEINS; CYSTEINE; CYTOCHALASIN B; DNA PRIMERS; EOSINE YELLOWISH-(YS); ETHYLMALEIMIDE; FORSKOLIN; GALACTOSE; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; PERIPLASMIC BINDING PROTEINS; PROTEIN BINDING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035252888     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3530709     Document Type: Article

References (58)

1. The cloning, DNA-sequence and overexpression of the gene araE coding for arabinose-proton symport in Escherichia coli K12
2. Mammalian and bacterial sugar-transport proteins are homologous
3. Homologies between sugar transporters from eukaryotes and prokaryotes
4. Homologous sugar-transport proteins in Escherichla coli and their relatives in both prokaryotes and eukaryotes Philos
5. Proton-linked sugar-transport systems in bacteria
6. Sugar cation symport systems in bacteria
7. Evolution of transmembrane transport: Relationships between transport proteins for sugars, carboxylate compounds, antibiotics and antiseptics
8. The 12-transmembrane helix transporters
9. Sequence and structure of a human glucose transporter
10. Structural requirements for binding to the sugar transport system of the human erythrocyte
11. Hydrogen bonding requirements for the insulin-sensitive sugar transport system of rat adipocytes
12. + transporter of Eschenchia coli
13. + symport protein of Escherichia coli
14. Inhibition of sugar transport in the human erythrocyte by cytochalasin B
15. The kinetics and thermodynamics of the binding of cytochalasin B to sugar transporters
16. + transport protein, GalP, of Escherichia coli
17. + transport protein, GalP
18. Cytochalasin B binding sites and glucose transport carrier in human erythrocyte ghosts
19. Inhibition of 3-methylglucose transport in human erythrocytes by forskolin
20. Activation of adenylate-cyclase and inhibition of glucose transport in rat adipocytes by forskolin analogues - Structural determinants for distinct sites of action
21. + symporter
22. Identification of the Ga1P galactose transport protein of Escherichia coli
23. Identification of the AraE transport protein of Escherichia coli
24. Assay, genetics, proteins and reconstitution of proton-linked galactose, arabinose and xylose transport systems of Escherichia coli
25. Inhibition of the glucose permeability of human erythrocytes by N-ethyl maleimide
26. Substrate-induced conformational change of human-erythrocyte glucose transporter - Inactivation by alkylating reagents
27. The cloning and DNA-sequence of the gene xylE for xylose-proton symport in Eschenchia coli K12
28. Sequence and structure of a human glucose transporter
29. The transport of galactose, glucose and their molecular analogues by Escherichia coli K12
30. A rapid, sensitive and specific method for the determination of protein in dilute solution
31. Experimental systems for the study of active transport in bacteria
32. The effect of osmotic shock on the accessibility of the murein layer of exponentially growing Escherichia coli to lysozyme
33. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli
34. Mechanisms of active transport in isolated membrane vesicles
35. Orientation of membrane vesicles from Escherichia coli prepared by different procedures
36. Orientation of membrane vesicles from Eschenchia coli as detected by freeze-cleave electron microscopy
37. Localisation of D-lactate dehydrogenase in membrane vesicles prepared by using a French Press or EDTA-lysozyme from Escherichia coli
38. Determination of the absolute number of Escherichia coli membrane vesicles that catalyze active transport
39. Localisation of D-lactate dehydrogenase in native and reconstituted Escherichia coli vesicles
40. Mechanism of assembly of the outer membrane of Salmonella typhimurium
41. Statistical analysis of enzyme kinetic data
42. + symport protein (Ga1P) from Escherichia coli
43. + symport protein (Ga1P) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar
44. Photolabeling of the hexose transporter at external and internal sites - Fragmentation patterns and evidence for a conformational change
45. Investigation of the structure and function of the human-erythrocyte glucose transporter by proteolytic dissection
46. Localization of the forskolin photolabeling site within the monosaccharide transporter of human erythrocytes
47. The glucose-transport activity of glut1 is markedly decreased by substitution of a single amino-acid with a different charge at residue-415
48. Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin-B photolabelling of the glut1 glucose transporter
49. Characterisation of purified, reconstituted site-directed cysteine mutants of the lactose permease of Escherichia coli
50. Construction of a functional lactose permease devoid of cysteine residues
51. A molecular mechanism for energy coupling in a membrane transport protein, the lactose permease of Escherichia coli
52. The role of cysteine residues in glucose-transporter-GLUT1-mediated transport and transport inhibition
53. Identification of an amino acid residue that lies between the exofacial vestibule and exofacial substrate-binding site o1 the Glut1 sugar permeation pathway
54. Proton-linked L-rhamnose transport and its comparison with L-fucose transport, in Escherichia coli
55. Rotational mobility and orientational stability of a transport protein in lipid membranes
56. Structural information on a membrane transport protein from nuclear magnetic resonance using sequence-selective nitroxide labelling