A PDZ domain-based detection system for enzymatic assays

Analytical Biochemistry

Volumn 301, Issue 2, 2002, Pages 207-216

  Ferrer, Marc ^a   Hamilton, Aaron C ^a   Inglese, James ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Enzymatic reactions; PSD95 disks large ZO 1 (PDZ); Time resolved fluorescence resonance energy transfer

Indexed keywords

BINDING ENERGY; COMPLEXATION; ENERGY TRANSFER; ENZYMES; FORSTER RESONANCE ENERGY TRANSFER; LIGANDS;

C-TERMINAL EXTENSIONS; CONSENSUS SEQUENCE; DETECTION METHODS; ENZYMATIC REACTION; ENZYME SUBSTRATES; GLUTATHIONE S-TRANSFERASES; PSD95/DISKS-LARGE/ZO-1 (PDZ); TIME-RESOLVED FLUORESCENCE;

PEPTIDES;

ALLOPHYCOCYANIN; ENDOPROTEASE; EUROPIUM; EXOPEPTIDASE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PDZ PROTEIN; PHOSPHATASE; PROTEINASE; SERINE; SERINE THREONINE PHOSPHATASE; STREPTAVIDIN; UNCLASSIFIED DRUG; VALINE;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CHELATION; COMPLEX FORMATION; CONSENSUS SEQUENCE; ENERGY TRANSFER; ENZYME ASSAY; ENZYME SUBSTRATE; EPITOPE MAPPING; FLUORESCENCE; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING;

EID: 0037083554     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5497     Document Type: Article

References (30)

1. Making artificial antibodies: A format for phage display of combinatorial heterodimeric arrays
2. Protein-protein interactions define specificity in signal transduction
3. PDZ domains: Fundamental building blocks in the organization of protein complexes at the plasma membrane
4. Peptide-surface associations: The case of PDZ and PTB domains
5. Recognition of unique carboxyl-terminal motifs by distinct PDZ domains
6. PDZ domain of neuronal nitric oxide synthase recognizes novel C-terminal peptide sequences
7. Crystal structures of a complexed and peptide-free membrane protein-binding domain: Molecular basis of peptide recognition by PDZ
8. Ligand-receptor binding measured by laser-scanning imaging
9. Presenilin 1 is linked with γ-secretase activity in the detergent solubilized state
10. Time-resolved fluorometry: An overview of the labels and core technologies for drug screening
11. Tyrosine kinase assays adapted to homogeneous time-resolved fluorescence
12. Single-step purification of polypeptides expressed in Escherichia coli as fusions with gluthathione S-transferase
13. Carboxypeptidase B (porcine pancreas)
14. HIV protease as a target for the design of antiviral agents for AIDS
15. Purification and properties of a carboxypeptidase inhibitor from potatoes
16. An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues
17. Cantharidin-binding protein: Identification as protein phosphatase 2A
18. Use of a phosphotyrosine-antibody pair as a general detection method in homogeneous time-resolved fluorescence: Application to human immunodeficiency viral protease
19. + exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains
20. Crystal structure of the hCASK PDZ domain reveals the structural basis of class II PDZ domain target recogntion
21. A cumulative specificity model for proteases from human immunodeficiency virus types 1 and 2, inferred from statistical analysis of an extended substrate data base
22. + exchanger regulatory factor family of PDZ proteins
23. 2-adrenergic receptor
24. + channel Kir 2.3 to PSD-95 is regulated by protein kinase A phosphorylation
25. The carboxyl terminus of B class ephrins constitutes a PDZ domain binding motif
26. Peptide binding consensus of the NHE-RF-PDZ1 domain matches the C-terminal sequence of cystic fibrosis transmembrane conductance regulator (CFTR)
27. Analysis of PDZ domain-ligand interactions using carboxyl-terminal phage display
28. Rapid identification of substrates for novel proteases using a combinatorial peptide library
29. Mutagenesis and selection of PDZ domains that bind new protein targets
30. Single-amino acid substitutions alter the specificity and affinity of PDZ domains for their ligands