Rapid identification of substrates for novel proteases using a combinatorial peptide library

Journal of Combinatorial Chemistry

Volumn 2, Issue 5, 2000, Pages 461-466

  Rossé, Gérard ^a,c   Kueng, Erich ^c   Page, Malcolm G P ^c   Schauer Vukasinovic, Vesna ^c   Giller, Thomas ^c   Lahm, Hans Werner ^c   Hunziker, Peter ^d   Schlatter, Daniel ^b,c  

^a Selectide Corporation   (United States)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

^c F HOFFMANN LA ROCHE LTD   (Switzerland)

^d UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; ISOQUINOLINE DERIVATIVE; LUCIFER YELLOW; MEMBRANE PROTEIN; OLIGOPEPTIDE; PEPTIDE LIBRARY; PROTEINASE; SERINE PROTEINASE; TYPE I SIGNAL PEPTIDASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COMBINATORIAL CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; KINETICS; METABOLISM; METHODOLOGY; REPRODUCIBILITY; SYNTHESIS;

AMINO ACID SEQUENCE; COMBINATORIAL CHEMISTRY TECHNIQUES; ENDOPEPTIDASES; ESCHERICHIA COLI; FLUORESCENT DYES; ISOQUINOLINES; KINETICS; MEMBRANE PROTEINS; OLIGOPEPTIDES; PEPTIDE LIBRARY; REPRODUCIBILITY OF RESULTS; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY;

EID: 0034263761     PISSN: 15204766     EISSN: None     Source Type: Journal    
DOI: 10.1021/cc000019y     Document Type: Article

References (36)

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34. One reviewer of this manuscript cautioned that the use of lysates, and not purified proteases, might cause investigators to pursue false positives. We thank this reviewer for the cautionary note.
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