(+)-(10R)-germacrene A synthase from goldenrod, Solidago canadensis; cDNA isolation, bacterial expression and functional analysis

Phytochemistry

Volumn 60, Issue 7, 2002, Pages 691-702

  Prosser, Ian ^a   Phillips, Andy L ^a   Gittings, Simon ^a   Lewis, Mervyn J ^a   Hooper, Antony M ^b   Pickett, John A ^b   Beale, Michael H ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Farnesyl diphosphate; Germacrene A; Goldenrod; Sesquiterpene synthase; Solidago canadensis

Indexed keywords

COMPLEMENTARY DNA; GERMACRENE A SYNTHASE; PRIMER DNA; SOLIDAGO CANADENSIS EXTRACT; TRANSFERASE; UNCLASSIFIED DRUG; GERMACRENE A SYNTHASE, CICHORIUM INTYBUS; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM EXAMINATION; CHEMISTRY; CYCLIZATION; CYTOTOXICITY; DNA ISOLATION; ENZYME SUBSTRATE; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; MASS FRAGMENTOGRAPHY; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FOLDING; REACTION ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SOLIDAGO;

ALKYL AND ARYL TRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; DNA PRIMERS; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS FRAGMENTOGRAPHY; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; SOLIDAGO; SUPPORT, NON-U.S. GOV'T; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; PLANT PROTEINS;

BACTERIA (MICROORGANISMS); NICOTIANA TABACUM; SOLIDAGO; SOLIDAGO CANADENSIS;

EID: 0037077411     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0031-9422(02)00165-6     Document Type: Article

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