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note
-
For clarity, numbering in dihydrogermacrene A (6) has been based on numbering of the corresponding carbon atoms in the precursor dihy-drofarnesyl diphosphate (5).
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note
-
In preliminary incubations, (7R)-6,7-dihydrofarnesyl diphosphate (5) was turned over by aristolochene synthase about twice as efficiently as was the corresponding (7S)-6,7-dihydrofarnesyl diphosphate. For this reason we decided to concentrate our efforts on the 7R enantiomer.
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10344258256
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unpublished
-
We cannot as yet rule out aberrant binding of dihydrofarnesyl diphosphate so as to generate dihydrogermacrene A with the 2-propenyl group in the unnatural configuration. Several derivatives of synthetic 6b have been prepared, but to date none have been suitable for X-ray crystallographic analysis (D. E. Cane and S. Tsubotani, unpublished).
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