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0011252551
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note
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Cys 110 on the surface of human Mb was changed to alanine to improve the yield upon purification; this mutation does not perturb ligand binding properties. See ref 29.
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0011174435
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note
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Soret = 160 OD/mM cm). (See ref 29.).
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32
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0025190014
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33
-
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0011251494
-
-
note
-
anh is dominant in the VSE for acetone, MVK, and NMP (see the results).
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-
-
-
34
-
-
0011219379
-
-
note
-
12 (data not shown). It is difficult to assign the overtone band of the carbonyl stretch for NMP because of a broader band and resonance mixing with other bands.
-
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36
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18144432446
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0011171374
-
-
note
-
Because CO is not very soluble in 2MeTHF and the extinction coefficient is small, the intensity of the absorption band for free CO dissolved in frozen 2MeTHF is much lower than other vibrations observed in this paper. Because of its low absorption intensity and small |Δμ|, the free CO data is much noisier.
-
-
-
-
42
-
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0011214806
-
-
note
-
Note that the observed values of |Δμ| are not corrected for the local field correction factor f. However, f is expected to be slightly larger than 1 and not to affect |Δμ| significantly. See ref 21 for details.
-
-
-
-
43
-
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0011179003
-
-
note
-
The local field correction factor is assumed to be unity. See ref 21.
-
-
-
-
44
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0035846626
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0001652725
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Kitagawa, T.5
-
46
-
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0011174436
-
-
note
-
Here, we are assuming that the protein electric field near the Fe-CO unit is fairly uniform, so that the magnitude and the direction of the local electric fields on C-O and Fe-C (or N-O and Fe-N) are the same.
-
-
-
-
47
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0033574735
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Kachalova, G.S.; Popov, A.N.; Bartunik, H.D. Science 1999, 284, 473.
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Popov, A.N.2
Bartunik, H.D.3
-
48
-
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0029078465
-
-
anh for the Fe-C stretch could be obtained experimentally if the IR intensity of the mode were measured; however, because of its lower stretch frequency, it is difficult to observe the Fe-CO stretch by IR.
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(1995)
Proc. Natl. Acad. Sci. U.S.A.
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-
-
Wang1
-
51
-
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0011180206
-
-
note
-
15N stretch band, respectively.
-
-
-
-
52
-
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0011219380
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-
Unpublished data
-
Unpublished data.
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-
|