Versatile method for production and controlled polymer-immobilization of biologically active recombinant proteins

Biotechnology and Bioengineering

Volumn 80, Issue 3, 2002, Pages 341-348

  Allard, Laure ^a   Cheynet, Valérie ^a   Oriol, Guy ^a   Mandrand, Bernard ^a   Delair, Thierry ^a   Mallet, François ^a  

^a BIOMÉRIEUX   (France)

Author keywords

ELISA; HIV 1 p24 capsid protein; Optimal biological activity; Oriented immobilization of proteins; Versatile expression vectors

Indexed keywords

AMINES; ANTIBODIES; BIOASSAY; COPOLYMERS;

DIAGNOSTIC CONTEXTS;

BIOTECHNOLOGY;

AMINE; ANTIGEN P24; COPOLYMER; HISTIDINE; LYSINE; MALEIC ANHYDRIDE; METAL CHELATE; POLYMER; PYRAN COPOLYMER; RECOMBINANT PROTEIN;

ANTIBODY TITER; ARTICLE; CONTROLLED STUDY; COVALENT BOND; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MAJOR CLINICAL STUDY; PROTEIN IMMOBILIZATION; SEROCONVERSION; SERODIAGNOSIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HIV CORE PROTEIN P24; HUMANS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; POLYMERS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE TAGGED SITES;

HUMAN IMMUNODEFICIENCY VIRUS 1; VECTORS;

EID: 0037027405     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10390     Document Type: Article

References (19)

1. Allard L, Cheynet V. Oriol L, Véron F, Merlier G, Scrémin G, Mandrand B, Delair T, Mallet F. 2001. Mechanisms leading to an oriented immobilization of recombinant proteins derived from the p24 capsid of HIV-1 onto copolymers. Bioconjug Chem 12:972-979.
2. Arnaud N, Cheynet V, Oriol G, Mandrand B, Mallet F. 1997. Construction and expression of a modular gene encoding bacteriophage T7 RNA polymerase. Gene 199:149-156.
3. Ausubel FM, Brent R, Kingstion RE, Moore DD, Seidman JG, Smith JA, Struhl K. 1993. Current Protocols in Molecular Biology. New York: John Wiley & Sons.
4. Berthet-Colominas C, Monaco S, Novelli A, Sibaï G, Mallet F, Cusack S. 1999. Head-to-tail dimers and interdomain flexibility revealed by the crystal structure of HIV-1 capsid protein (p24) complexed with a monoclonal antibody Fab. EMBO 18:1124-1136.
5. Betemps D, Mallet F, Cheynet V, Baron T. 1999. Overexpression and purification of an immunologically reactive His-BIV capsid fusion protein. Prot Express Purif 15:258-264.
6. Cheynet V, Verrier B, Mallet F. 1993. Overexpression of HIV-1 proteins in Escherichia coli by a modified expression vector and their one-step purification. Prot Express Purif 4:367-372.
7. Chilkoti A, Chen G, Stayton PS, Hoffman AS. 1994. Site-specific conjugation of a temperature-sensitive polymer to a genetically engineered protein. Bioconjug Chem 5:504-507.
8. Delair T, Ladavière C, Domard A, Novelli-Rousseau A, Mandrand B, Mallet F. (2000). Electrostatically driven conjugation of proteins onto copolymers: Toward a regio selective reaction. Polymer Preprints 41:1023.
9. Duncan R. 1999. Polymer conjugates for antitumor targeting and intracytoplasmic delivery. The EPR effect as a common gateway? PSTT 2:441-449.
10. Ganachaud F, Mouterde G, Delair Th, Elaïssari A, Pichot C. 1995. Preparation and characterization of cationic polystyrene latex particles of different aminated surface charges. Polymers Adv Technol 6:480-488.
11. Greiner M, Pfeiffer D, Smith RD. 2000. Principles and practical application of the receiver-operating characteristic analysis for diagnostic test. Prevent Vet Med 45:23-41.
12. Isosaki K, Seno N, Matsumoto I. 1992. Immobilization of protein ligands with methyl vinyl ether-maleic anhydride copolymer. J Chromatogr 597:123-128.
13. Janvier B, Mallet F, Cheynet V, Dalbon P, Vernet G, Besnier JM, Choutet JP, Goudeau A, Mandrand B, Barin F. 1993. Prevalence and persistance of antibody titers to recombinant HIV-1 core and matrix proteins in HIV-1 infection. J Acquir Immune Defic Syndr 6:898-903.
14. Ladavière C, Delair T, Domard A, Novelli-Rousseau A, Mandrand B, Mallet F. 1998. Covalent immobilization of proteins onto (maleic anhydride-alt-methyl vinyl ether) copolymers: enhanced immobilization of recombinant proteins. Bioconjug Chem 9:655-661.
15. Lefrere JJ, Courouce AM, Lucotte G, Boitard C, Kaplan C, Nicolas JC, Bricout F, Lambin P, Doinel C, Muller JY. 1998. Follow-up of subjects with isolated and persistent anti-core (anti-p24 or anti-p17) antibodies to HIV. AIDS 2:287-290.
16. Monfardini A, Veronese FM. 1998. Stabilization of substances in circulation. Bioconjug Chem 9:418-450.
17. Rao SV, Anderson KW, Bachas LG. 1998. Oriented immobilization of proteins. Mikrochim Acta 128:127-143.
18. Stayton PS, Fischer MT, Sligar SG. 1988. Determination of cytochrome b5 association reactions. Characterization of methemoglobin and cytochrome P-450cam bind-ing to genetically engineered cytochrome b5. J Biol Chem 263:13544-13548.
19. Varga CM, Wickham TJ, Lauffenburger DA. 2000. Receptor-mediated targeting of gene delivery vectors: Insights from molecular mechanisms for improved vehicle design. Biotechnol Bioeng 70:593-605.