Head-to-tail dimers and interdomain flexibility revealed by the crystal structure of HIV-1 capsid protein (p24) complexed with a monoclonal antibody Fab

EMBO Journal

Volumn 18, Issue 5, 1999, Pages 1124-1136

  Berthet Colominas, Carmen ^a   Monaco, Stéphanie ^a   Novelli, Armelle ^b   Sibaï, Geneviève ^c   Mallet, François ^b   Cusack, Stephen ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (France)

^b UNIVERSITÉ DE LYON   (France)

^c BIOMÉRIEUX   (France)

Author keywords

Fab; HIV 1; p24; Virus assembly; X ray crystallography

Indexed keywords

CAPSID PROTEIN; DIMER; GAG PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; VIRUS ASSEMBLY;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; MIRIDAE; RNA VIRUSES;

EID: 0033106192     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.5.1124     Document Type: Article

References (32)

1. Accola, M.A., Höglund, S. and Göttlinger, H.G. (1998) A putative α-helical structure which overlaps the capsid-p2 boundary in the HIV-1 Gag precursor is crucial for viral particle assembly. J. Virol., 12, 2072-2078.
2. Brünger, T.A. (1992) X-PLOR version 3.1. Yale University Press, New Haven, CT, USA.
3. Cheynet, V., Verrier, B. and Mallet, F. (1993) Overexpression of HIV-1 proteins in E.coli by a modified expression vector and their one-step purification. Protein Exp. Purif., 4, 367-372.
4. Collaborative Computational Project Number 4 (1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr., D50, 760-763.
5. Cusack, S., Belrhali, H., Bram, A., Burghammer, M., Perrakis, A. and Riekel, C. (1998) Small is beautiful: protein microcrystallography. Nature Struct. Biol. (Synchrotron Suppl.), 5, 634-637.
6. Dorfman, T., Bukovsky, A., Öhagen, Å., Höglund, S. and Göttlinger, H.G. (1994) Functional domains of the capsid protein of HIV-1. J. Virol., 68, 8180-8187.
7. Franke, E.K., Yuan, H.E.H. and Luban, J. (1994) Specific incorporation of cyclophilin A into HIV-1 virions. Nature, 372, 359-362.
8. Freed, E.O. (1998) HIV-1 Gag proteins: diverse functions in the virus life cycle. Virology, 251, 1-15.
9. Fuller, S.D., Wilk, T., Gowen, B.E., Kräusslich, H.-G. and Vogt, V.M. (1997) Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle. Curr. Biol., 7, 729-738.
10. Gamble, T.R., Vajdos, F.F., Yoo, S., Worthylake, D.K., Housesweart, M., Sundquist, W.I. and Hill, C.P. (1996) Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid. Cell, 87, 1285-1294.
11. Gamble, T.R., Yoo, S., Vajdos, F.F., Schwedler, U.K., Worthylake, D.K., Wang, H., McCutcheon, J.P., Sundquist, W.I. and Hill, C.P. (1997) Structure of the carboxyl-terminal dimerisation domain of the HIV-1 capsid protein. Science, 278, 849-853.
12. Garnier, L., Ratner, L., Rovinski, B., Cao, S.-X. and Wills, J.W. (1998) Particle size determinants in the human immunodeficiency virus type 1 Gag protein. J. Virol., 72, 4667-4677.
13. Gitti, R.K., Lee, B.M., Walker, J., Summers, M.F., Yoo, S. and Sundquist, W.I. (1996) Structure of the amino-terminal core domain of the HIV-1 capsid protein. Science, 273, 231-235.
14. Gross, I., Hohenberg, H. and Kräusslich, H.-G. (1997) In vitro assembly properties of purified bacterially expressed capsid proteins of human immunodeficiency virus. Eur. J. Biochem., 249, 592-600.
15. Janvier, B. et al. (1993) Prevalence and persistence of antibody titers to recombinant HIV-1 core and matrix proteins in HIV-1 infection. J. Acquir. Immune Defic. Syndr., 6, 898-903.
16. Jin, Z., Jin, L., Peterson, D.L. and Lawson, C.L. (1999) Model for lentivirus capsid core assembly based on crystal dimers of EIAV p26. J. Mol. Biol., 286, 83-93.
17. Kräusslich, H.-G. (1996) Morphogenesis and Maturation of Retroviruses. Current Topics in Immunology. Springer-Verlag, Berlin, Germany.
18. Kraulis, P.J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr., 24, 946-950.
19. Leslie, A.G.W. (1992) Joint CCP4 and ESF-EACBM Newsletter on Protein Crystallography, No. 26. Daresbury Laboratory, Warrington WA4 4AD, UK.
20. Luban, J. (1996) Absconding with the chaperone: essential cyclophilin-Gag interaction in HIV-1 virions. Cell, 87, 1157-1159.
21. Momany, C. et al. (1996) Crystal structure of dimeric HIV-1 capsid protein. Nature Struct. Biol., 3, 763-770.
22. Morikawa, Y., Zhang, W.-H., Hockley, D.J., Nermut, M.V. and Jones, I.M. (1998) Detection of a trimeric HIV-1 Gag intermediate is dependent on sequences in the matrix protein, p17. J. Virol., 72, 7659-7663.
23. Navaza, J. (1994) AmoRe: an automated package for molecular replacement. Acta Cryst., A50, 157-163.
24. Prongay, A.J. et al. (1990) Preparation and crystallisation of a HIV p24-Fab complex. Proc. Natl Acad. Sci. USA, 87, 9980-9984.
25. Read, R.J. (1986) Improved Fourier coefficients for maps using phases from partial structures with errors. Acta Cryst., A42, 140-149.
26. Reicin, A.S., Paik, S. and Goff, S.P. (1995) Linker insertion mutations in the HIV-1 Gag gene: effects on virion particle assembly, release and infectivity. J. Virol., 69, 642-650.
27. von Schwedler, U.K., Stemmler, T.L., Klishko, V.Y., Li, S., Albertine, K.H., Davis, D.R. and Sundquist, W.I. (1998) Proteolytic refolding of the HIV-1 capsid protein amino-terminus facilitates vial core assembly. EMBO J., 17, 1555-1568.
28. Thali, M., Bukovsky, A., Komdo, E., Rosenwirth, B., Walsh, C.T., Sodroski, J. and Göttlinger, H.G. (1994) Functional association of cyclophilin A with HIV-1 virions. Nature, 372, 363-365.
29. Wang, C.-T. and Barklis, E. (1993) Assembly, processing and infectivity of HIV-1 Gag mutants. J. Virol., 67, 4264-4273.
30. Wiegers, K., Rutter, G., Kottler, H., Tessmer, U., Hohenberg, H. and Kräusslich, H.-G. (1998) Sequential steps in HIV particle maturation revealed by alterations of individual Gag polyprotein cleavage sites. J. Virol., 72, 2846-2854.
31. Yeager, M., Wilson-Kubalek, E.M., Weiner, S.G., Brown, P.O. and Rein, A. (1998) Supramolecular organisation of immature and mature murine leukemia virus revealed by electron cryo-microscopy: implications for retroviral assembly mechanisms. Proc. Natl Acad. Sci. USA, 95, 7299-7304.
32. Yoo, S., Myszka, D.G., Yeh, C., McMurray, M., Hill, C.P. and Sundquist, W.I. (1997). Molecular recognition in the HIV-1 capsid/cyclophilin A complex. J. Mol. Biol., 269, 780-795.