Cloning of the N-acylamino acid racemase gene from Amycolatopsis azurea and biochemical characterization of the gene product

Enzyme and Microbial Technology

Volumn 30, Issue 5, 2002, Pages 647-655

  Su, Shiun Cheng ^a   Lee, Chia Yin ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Enolase superfamily; N acylamino acid racemase; o succinylbenzoate synthase; Optically active amino acid

Indexed keywords

AMINO ACIDS; BACTERIA; CHROMATOGRAPHIC ANALYSIS; CLONING; MOLECULAR WEIGHT; ORGANIC COMPOUNDS; PH EFFECTS; PROTEINS;

GEL-FILTRATION CHROMATOGRAPHY;

GENES;

AMINO ACID DERIVATIVE; COBALT; GENE PRODUCT; MANGANESE; N ACETYLMETHIONINE; N ACETYLTYROSINE; N ACETYLVALINE; N ACYLAMINO ACID; N CHLOROACETYL DEXTRO PHENYLALANINE; RACEMASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ACTINOMYCES; AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; AMYCOLATOPSIS AZUREA; ARTICLE; BACTERIAL STRAIN; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SUBUNIT; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL FILTRATION CHROMATOGRAPHY; GENE IDENTIFICATION; GENETIC CODE; GENETIC TRANSFORMATION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE;

AMYCOLATOPSIS; AMYCOLATOPSIS AZUREA; AMYCOLATOPSIS SP.; VECTORS;

EID: 0037007545     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00026-1     Document Type: Article

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