Single-molecule imaging and spectroscopy using fluorescence and surface-enhanced Raman scattering

Journal of Biological Physics

Volumn 28, Issue 4, 2002, Pages 573-585

  Ishikawa, M ^a   Maruyama, Y ^a   Ye, J Y ^a   Futamata, M ^b  

^a JT RES CENTER FOR ATOM TECHNOLOGY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Adenine; Fluorescence; Klenow fragment; Silver particles; Single molecule imaging; Surface enhanced Raman scattering; TNP ATP

Indexed keywords

ADENINE DERIVATIVE; NANOPARTICLE; NUCLEIC ACID BASE; SILVER;

COLLOID; CONFERENCE PAPER; ESCHERICHIA COLI; FLUORESCENCE; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; RAMAN SPECTROMETRY; STRUCTURE ANALYSIS;

ESCHERICHIA COLI; VIOLA (ANGIOSPERM);

EID: 0036952550     PISSN: 00920606     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1021222318740     Document Type: Article

References (27)

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8. This statement is simply based on our feeling with accumulated experience. Here we have no rigorous discussion about what factors determine signal-to-noise ratio of single-molecule fluorescence.
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20. A previous study (Kneipp, K., Kneipp, H., Kartha, B., Manoharan, R., Deinum, G., Itzkan, I., Dasari, R.R. and Feld, M.S.: Detection and Identification of a Single DNA Base Molecule Using Surface-Enhanced Raman Scattering (SERS), Phys. Rev. E57 (1998), 86281-86284) reported single-molecule detection and spectroscopy of adenine. This study, however, like References 16 and 18 detected SERS intensities of adenine on SERS-active hot particles subject to Brownian movement in a probe volume in solution using burst detection technique. Without imaging technique, no one identifies where are hot particles and what percentages of all particles are hot particles. Moreover, the temporal trajectory of SERS intensities on a single hot particle cannot be obtained by using burst detection technique.
21. The intensity of SERS showing blinking was constant witkin a factor of ∼3 irrespective of the difference in the average number of R6G molecules on a particle by a factor of 10 (0.3 and 3).
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26. -6 M or higher concentrations. No these unexpected phenomena were observed for R6G.
27. To increase the number of cationic adenine molecules, the pH of the solution was changed. Unfortunately under acidic conditions (pH = 3-4) Ag particles formed precipitations, which are much larger than hot particles composed of aggregated particles. Thus, it is impossible to implement SERS experiments using acidic solutions as they are.