Construction of DNA-shuffled and incrementally truncated libraries by a mutagenic and unidirectional reassembly method: Changing from a substrate specificity of phospholipase to that of lipase

Applied and Environmental Microbiology

Volumn 68, Issue 12, 2002, Pages 6146-6151

  Song, Jae Kwang ^a,b   Chung, Bora ^a   Oh, Young Hak ^a   Rhee, Joon Shick ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Chemical Technology   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; DNA SEQUENCES; ENZYMES; MICROBIOLOGY;

MUTAGENIC AND UNIDIRECTIONAL ASSEMBLY (MURA);

MUTAGENESIS;

DEOXYRIBONUCLEASE I; DNA POLYMERASE; PHOSPHOLIPASE; RESTRICTION ENDONUCLEASE; TRIACYLGLYCEROL LIPASE;

ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; DNA LIBRARY; DNA SEQUENCE; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME SPECIFICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE; SERRATIA;

DNA SHUFFLING; GENE LIBRARY; LIPASE; MUTAGENESIS; PHOSPHOLIPASES A; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; SERRATIA; SUBSTRATE SPECIFICITY;

NEGIBACTERIA; SERRATIA; SERRATIA SP.;

EID: 0036951893     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.12.6146-6151.2002     Document Type: Article

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