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Volumn 50, Issue 12, 2002, Pages 1633-1640

Evaluation of thymidylate synthase protein expression by western blotting and immunohistochemistry on human colon carcinoma xenografts in nude mice

Author keywords

Cell kinetics; Colorectal cancer; Immunohistochemistry; Nude mice; Silver stained nucleolus; Thymidylate synthase; Tumor growth rate; Western blotting

Indexed keywords

MONOCLONAL ANTIBODY; PROTEIN P53; THYMIDYLATE SYNTHASE;

EID: 0036939857     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540205001207     Document Type: Article
Times cited : (9)

References (38)
  • 1
    • 0036534002 scopus 로고    scopus 로고
    • Investigation of the prognostic and predictive value of thymidylate synthase, p53, and Ki-67 in patients with locally advanced colon cancer
    • Allegra CJ, Parr AL, Wold LE, Mahoney MR, Sargent DJ, Johnston P, Klein P, et al. (2002) Investigation of the prognostic and predictive value of thymidylate synthase, p53, and Ki-67 in patients with locally advanced colon cancer. J Clin Oncol 20:1735-1743
    • (2002) J Clin Oncol , vol.20 , pp. 1735-1743
    • Allegra, C.J.1    Parr, A.L.2    Wold, L.E.3    Mahoney, M.R.4    Sargent, D.J.5    Johnston, P.6    Klein, P.7
  • 2
    • 17744364074 scopus 로고    scopus 로고
    • Expression of thymidylate synthase in gastric cancer patients treated with 5-fluorouracil and doxorubicin-based adjuvant chemotherapy after curative resection
    • Choi J, Lim H, Nam DK, Kim HS, Cho DY, Yi JW, Kim HC, et al. (2001) Expression of thymidylate synthase in gastric cancer patients treated with 5-fluorouracil and doxorubicin-based adjuvant chemotherapy after curative resection. Br J Cancer 84:186-192
    • (2001) Br J Cancer , vol.84 , pp. 186-192
    • Choi, J.1    Lim, H.2    Nam, D.K.3    Kim, H.S.4    Cho, D.Y.5    Yi, J.W.6    Kim, H.C.7
  • 3
    • 0026096558 scopus 로고
    • Alpha 2,6 sialylation of N-acetyllactosaminic sequences in human colorectal cancer cell lines. Relationship with non-adherent growth
    • Dall'Olio F, Malagolini N, Di Stefano G, Ciambella M, SerafiniCessi F (1991) Alpha 2,6 sialylation of N-acetyllactosaminic sequences in human colorectal cancer cell lines. Relationship with non-adherent growth. Int J Cancer 47:291-297
    • (1991) Int J Cancer , vol.47 , pp. 291-297
    • Dall'Olio, F.1    Malagolini, N.2    Di Stefano, G.3    Ciambella, M.4    SerafiniCessi, F.5
  • 4
    • 0017716458 scopus 로고
    • Thymidylate synthetase - A target enzyme in cancer chemotherapy
    • Danenberg PV (1977) Thymidylate synthetase - a target enzyme in cancer chemotherapy. Biochim Biophys Acta 473:73-92
    • (1977) Biochim Biophys Acta , vol.473 , pp. 73-92
    • Danenberg, P.V.1
  • 5
    • 0015993325 scopus 로고
    • Structures of reversible and irreversible complexes of thymidylate synthetase and fluorinated pyrimidine nucleotides
    • Danenberg PV, Langenbach RJ, Heidelberger C (1974) Structures of reversible and irreversible complexes of thymidylate synthetase and fluorinated pyrimidine nucleotides. Biochemistry 13:926-933
    • (1974) Biochemistry , vol.13 , pp. 926-933
    • Danenberg, P.V.1    Langenbach, R.J.2    Heidelberger, C.3
  • 7
    • 85036692789 scopus 로고    scopus 로고
    • Thymidylate synthase protein expression and activity are related to cell proliferation rate in human cancer cell lines
    • in press
    • Derenzini M, Montanaro L, Treré D, Chillà A, Tazzari PL, Dall'Olio F, Ofner D (in press) Thymidylate synthase protein expression and activity are related to cell proliferation rate in human cancer cell lines. Mol Pathol
    • Mol Pathol
    • Derenzini, M.1    Montanaro, L.2    Treré, D.3    Chillà, A.4    Tazzari, P.L.5    Dall'Olio, F.6    Ofner, D.7
  • 8
    • 0034114866 scopus 로고    scopus 로고
    • Nucleolar size indicates the rapidity of cell proliferation in cancer tissues
    • Derenzini M, Treré D, Pession A, Govoni M, Sirri V, Chieco P (2000) Nucleolar size indicates the rapidity of cell proliferation in cancer tissues. J Pathol 191:181-186
    • (2000) J Pathol , vol.191 , pp. 181-186
    • Derenzini, M.1    Treré, D.2    Pession, A.3    Govoni, M.4    Sirri, V.5    Chieco, P.6
  • 10
    • 0036534108 scopus 로고    scopus 로고
    • Thymidylate synthase expression in colorectal cancer: A prognostic and predictive marker of benefit from adjuvant fluorouracil-based chemotherapy
    • Edler D, Glimelius B, Hallstrom M, Jakobsen A, Johnston PG, Magnusson I, Ragnhammar P, et al. (2002) Thymidylate synthase expression in colorectal cancer: a prognostic and predictive marker of benefit from adjuvant fluorouracil-based chemotherapy. J Clin Oncol 20:1721-1728
    • (2002) J Clin Oncol , vol.20 , pp. 1721-1728
    • Edler, D.1    Glimelius, B.2    Hallstrom, M.3    Jakobsen, A.4    Johnston, P.G.5    Magnusson, I.6    Ragnhammar, P.7
  • 11
    • 0034087575 scopus 로고    scopus 로고
    • Thymidylate synthase expression: An independent prognostic factor for local recurrence, distant metastasis, disease-free and overall survival in rectal cancer
    • Edler D, Hallstrom M, Johnston PG, Magnusson I, Ragnhammar P, Blomgren H (2000a) Thymidylate synthase expression: an independent prognostic factor for local recurrence, distant metastasis, disease-free and overall survival in rectal cancer. Clin Cancer Res 6:1378-1384
    • (2000) Clin Cancer Res , vol.6 , pp. 1378-1384
    • Edler, D.1    Hallstrom, M.2    Johnston, P.G.3    Magnusson, I.4    Ragnhammar, P.5    Blomgren, H.6
  • 12
    • 0033981858 scopus 로고    scopus 로고
    • Immunohistochemically detected thymidylate synthase in colorectal cancer: An independent prognostic factor of survival
    • Edler D, Kressner U, Ragnhammar P, Johnston PG, Magnusson I, Glimelius B, Pahlman L, et al. (2000b) Immunohistochemically detected thymidylate synthase in colorectal cancer: an independent prognostic factor of survival. Clin Cancer Res 6:488-492
    • (2000) Clin Cancer Res , vol.6 , pp. 488-492
    • Edler, D.1    Kressner, U.2    Ragnhammar, P.3    Johnston, P.G.4    Magnusson, I.5    Glimelius, B.6    Pahlman, L.7
  • 14
    • 0030000639 scopus 로고    scopus 로고
    • Cytometric measurement of cell proliferation in echo-guided biopsies from focal lesions of the liver
    • Faccioli S, Chieco P, Gramantieri L, Stecca BA, Bolondi L (1996) Cytometric measurement of cell proliferation in echo-guided biopsies from focal lesions of the liver. Mod Pathol 9:120-125
    • (1996) Mod Pathol , vol.9 , pp. 120-125
    • Faccioli, S.1    Chieco, P.2    Gramantieri, L.3    Stecca, B.A.4    Bolondi, L.5
  • 15
    • 0033824440 scopus 로고    scopus 로고
    • Thymidylate synthase expression in epithelial ovarian cancer: Relationship with thymidine phosphorylase expression and prognosis
    • Fujiwaki R, Hata K, Nakayama K, Fukumoto M, Miyazaki K (2000) Thymidylate synthase expression in epithelial ovarian cancer: relationship with thymidine phosphorylase expression and prognosis. Oncology 59:152-157
    • (2000) Oncology , vol.59 , pp. 152-157
    • Fujiwaki, R.1    Hata, K.2    Nakayama, K.3    Fukumoto, M.4    Miyazaki, K.5
  • 16
    • 0026795489 scopus 로고
    • Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines
    • Johnston PG, Drake JC, Trepel J, Allegra CJ (1992) Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines. Cancer Res 52:4306-4312
    • (1992) Cancer Res , vol.52 , pp. 4306-4312
    • Johnston, P.G.1    Drake, J.C.2    Trepel, J.3    Allegra, C.J.4
  • 17
    • 0028151449 scopus 로고
    • The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer
    • Johnston PG, Fisher ER, Rockette HE, Fisher B, Wolmark N, Drake JC, Chabner BA, et al. (1994) The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer. J Clin Oncol 12:2640-2647
    • (1994) J Clin Oncol , vol.12 , pp. 2640-2647
    • Johnston, P.G.1    Fisher, E.R.2    Rockette, H.E.3    Fisher, B.4    Wolmark, N.5    Drake, J.C.6    Chabner, B.A.7
  • 18
    • 0028940399 scopus 로고
    • Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors
    • Johnston PG, Lenz HJ, Leichman CG, Danenberg KD, Allegra CJ, Danenberg PV, et al. (1995) Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors. Cancer Res 55:1407-1412
    • (1995) Cancer Res , vol.55 , pp. 1407-1412
    • Johnston, P.G.1    Lenz, H.J.2    Leichman, C.G.3    Danenberg, K.D.4    Allegra, C.J.5    Danenberg, P.V.6
  • 19
    • 0026335113 scopus 로고
    • Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue
    • Johnston PG, Liang CM, Henry S, Chabner BA, Allegra CJ (1991) Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue. Cancer Res 51:6668-6676
    • (1991) Cancer Res , vol.51 , pp. 6668-6676
    • Johnston, P.G.1    Liang, C.M.2    Henry, S.3    Chabner, B.A.4    Allegra, C.J.5
  • 20
    • 0035671827 scopus 로고    scopus 로고
    • Different lengths of a polymorphic repeat sequence in the thymidylate synthase gene affect translational efficiency but not its gene expression
    • Kawakami K, Salonga D, Park JM, Danenberg KD, Uetake H, Brabender J, Omura K, et al. (2001) Different lengths of a polymorphic repeat sequence in the thymidylate synthase gene affect translational efficiency but not its gene expression. Clin Cancer Res 7:4096-4101
    • (2001) Clin Cancer Res , vol.7 , pp. 4096-4101
    • Kawakami, K.1    Salonga, D.2    Park, J.M.3    Danenberg, K.D.4    Uetake, H.5    Brabender, J.6    Omura, K.7
  • 21
    • 0026458517 scopus 로고
    • A non-organic and non-enzymatic extraction method gives higher yields of genomic DNA from whole-blood samples than do nine other methods tested
    • Lahiri DK, Bye S, Nurnberger JI Jr, Hodes ME, Crisp M (1992) A non-organic and non-enzymatic extraction method gives higher yields of genomic DNA from whole-blood samples than do nine other methods tested. J Biochem Biophys Methods 25:193-205
    • (1992) J Biochem Biophys Methods , vol.25 , pp. 193-205
    • Lahiri, D.K.1    Bye, S.2    Nurnberger J.I., Jr.3    Hodes, M.E.4    Crisp, M.5
  • 22
    • 0031213588 scopus 로고    scopus 로고
    • Inhibition of mouse thymidylate synthase promoter activity by the wild-type p53 tumor suppressor protein
    • Lee Y, Chen Y, Chang LS, Johnson LF (1997) Inhibition of mouse thymidylate synthase promoter activity by the wild-type p53 tumor suppressor protein. Exp Cell Res 234:270-276
    • (1997) Exp Cell Res , vol.234 , pp. 270-276
    • Lee, Y.1    Chen, Y.2    Chang, L.S.3    Johnson, L.F.4
  • 23
    • 14444285483 scopus 로고    scopus 로고
    • p53 point mutations and thymidylate synthase messenger RNA levels in disseminated colorectal cancer: An analysis of response and survival
    • Lenz HJ, Hayashi K, Salonga D, Danenberg KD, Danenberg PV, Metzger R, Banerjee D, et al. (1998) p53 point mutations and thymidylate synthase messenger RNA levels in disseminated colorectal cancer: an analysis of response and survival. Clin Cancer Res 4:1243-1250
    • (1998) Clin Cancer Res , vol.4 , pp. 1243-1250
    • Lenz, H.J.1    Hayashi, K.2    Salonga, D.3    Danenberg, K.D.4    Danenberg, P.V.5    Metzger, R.6    Banerjee, D.7
  • 24
    • 0035321315 scopus 로고    scopus 로고
    • Discrepancies between the gene expression, protein expression, and enzymatic activity of thymidylate synthase and dihydropyrimidine dehydrogenase in human gastrointestinal cancers and adjacent normal mucosa
    • Miyamoto S, Ochiai A, Boku N, Ohtsu A, Tahara M, Yoshida S, Okabe H, et al. (2001) Discrepancies between the gene expression, protein expression, and enzymatic activity of thymidylate synthase and dihydropyrimidine dehydrogenase in human gastrointestinal cancers and adjacent normal mucosa. Int J Oncol 18:705-713
    • (2001) Int J Oncol , vol.18 , pp. 705-713
    • Miyamoto, S.1    Ochiai, A.2    Boku, N.3    Ohtsu, A.4    Tahara, M.5    Yoshida, S.6    Okabe, H.7
  • 25
    • 0028351657 scopus 로고
    • Chemotherapy for colorectal cancer
    • Moertel CG (1994) Chemotherapy for colorectal cancer. N Engl J Med 330:1136-1142
    • (1994) N Engl J Med , vol.330 , pp. 1136-1142
    • Moertel, C.G.1
  • 26
    • 0019321438 scopus 로고
    • Cell cycle regulation of thymidylate synthetase gene expression in cultured mouse fibroblasts
    • Navaigund LG, Rossana C, Muench AJ, Johnson LF (1980) Cell cycle regulation of thymidylate synthetase gene expression in cultured mouse fibroblasts. J Biol Chem 255:7386-7390
    • (1980) J Biol Chem , vol.255 , pp. 7386-7390
    • Navaigund, L.G.1    Rossana, C.2    Muench, A.J.3    Johnson, L.F.4
  • 27
    • 0022637925 scopus 로고
    • Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level
    • Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet JJ (1986) Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J 18:5-14
    • (1986) Histochem J , vol.18 , pp. 5-14
    • Ploton, D.1    Menager, M.2    Jeannesson, P.3    Himber, G.4    Pigeon, F.5    Adnet, J.J.6
  • 29
    • 67049102265 scopus 로고
    • A biomathematical approach to clinical tumor growth
    • Swhartz MA (1961) A biomathematical approach to clinical tumor growth. Cancer 14:1272-1294
    • (1961) Cancer , vol.14 , pp. 1272-1294
    • Swhartz, M.A.1
  • 30
    • 0033995850 scopus 로고    scopus 로고
    • Relation between thymidylate synthase expression and survival in colon carcinoma, and determination of appropriate application of 5-fluorouracil by immunohistochemical method
    • Takenoue T, Nagawa H, Matsuda K, Fujii S, Nita ME, Hatano K, Kitayama J, et al. (2000) Relation between thymidylate synthase expression and survival in colon carcinoma, and determination of appropriate application of 5-fluorouracil by immunohistochemical method. Ann Surg Oncol 7:193-198
    • (2000) Ann Surg Oncol , vol.7 , pp. 193-198
    • Takenoue, T.1    Nagawa, H.2    Matsuda, K.3    Fujii, S.4    Nita, M.E.5    Hatano, K.6    Kitayama, J.7
  • 31
    • 0034174667 scopus 로고    scopus 로고
    • AgNOR staining and quantification
    • Treré D (2000) AgNOR staining and quantification. Micron 31:127-131
    • (2000) Micron , vol.31 , pp. 127-131
    • Treré, D.1
  • 32
    • 0033913428 scopus 로고    scopus 로고
    • Expression of thymidylate synthase in relation to survival and chemosensitivity in gastric cancer patients
    • Tsujitani S, Konishi I, Suzuki K, Oka S, Gomyo Y, Matsumoto S, Hirooka Y, et al. (2000) Expression of thymidylate synthase in relation to survival and chemosensitivity in gastric cancer patients. J Exp Clin Cancer Res 19:189-195
    • (2000) J Exp Clin Cancer Res , vol.19 , pp. 189-195
    • Tsujitani, S.1    Konishi, I.2    Suzuki, K.3    Oka, S.4    Gomyo, Y.5    Matsumoto, S.6    Hirooka, Y.7
  • 33
    • 18344408584 scopus 로고    scopus 로고
    • Thymidylate synthase expression in patients with colorectal carcinoma using a polyclonal thymidylate synthase antibody in comparison to the TS 106 monoclonal antibody
    • Van Triest B, Loftus BM, Pinedo HM, Backus HH, Schoenmakers P, Telleman F, Tadema T, et al. (2000a) Thymidylate synthase expression in patients with colorectal carcinoma using a polyclonal thymidylate synthase antibody in comparison to the TS 106 monoclonal antibody. J Histochem Cytochem 48:755-760
    • (2000) J Histochem Cytochem , vol.48 , pp. 755-760
    • Van Triest, B.1    Loftus, B.M.2    Pinedo, H.M.3    Backus, H.H.4    Schoenmakers, P.5    Telleman, F.6    Tadema, T.7
  • 34
    • 0032868050 scopus 로고    scopus 로고
    • Thymidylate synthase: A target for combination therapy and determinant of chemotherapeutic response in colorectal cancer
    • Van Triest B, Peters GJ (1999) Thymidylate synthase: a target for combination therapy and determinant of chemotherapeutic response in colorectal cancer. Oncology 57:179-194
    • (1999) Oncology , vol.57 , pp. 179-194
    • Van Triest, B.1    Peters, G.J.2
  • 35
    • 12944293119 scopus 로고    scopus 로고
    • Prognostic role of thymidylate synthase, thymidine phosphorylase/platelet-derived endothelial cell growth factor, and proliferation markers in colorectal cancer
    • Van Triest B, Pinedo HM, Blaauwgeers JL, van Diest PJ, Schoenmakers PS, Voorn DA, Smid K, et al. (2000b) Prognostic role of thymidylate synthase, thymidine phosphorylase/platelet-derived endothelial cell growth factor, and proliferation markers in colorectal cancer. Clin Cancer Res 6:1063-1072
    • (2000) Clin Cancer Res , vol.6 , pp. 1063-1072
    • Van Triest, B.1    Pinedo, H.M.2    Blaauwgeers, J.L.3    Van Diest, P.J.4    Schoenmakers, P.S.5    Voorn, D.A.6    Smid, K.7
  • 36
    • 0035868667 scopus 로고    scopus 로고
    • Polymorphisms of the repeated sequences in the enhancer region of the thymidylate synthase gene promoter may predict downstaging after preoperative chemoradiation in rectal cancer
    • Villafranca E, Okruzhnov Y, Dominguez MA, Garcia-Foncillas J, Azinovic I, Martinez E, Illarramendi JJ, et al. (2001) Polymorphisms of the repeated sequences in the enhancer region of the thymidylate synthase gene promoter may predict downstaging after preoperative chemoradiation in rectal cancer. J Clin Oncol 19:1779-1786
    • (2001) J Clin Oncol , vol.19 , pp. 1779-1786
    • Villafranca, E.1    Okruzhnov, Y.2    Dominguez, M.A.3    Garcia-Foncillas, J.4    Azinovic, I.5    Martinez, E.6    Illarramendi, J.J.7
  • 37
  • 38
    • 0031941249 scopus 로고    scopus 로고
    • A new prognostic factor for colorectal carcinoma, thymidylate synthase, and its therapeutic significance
    • Yamachika T, Nakanishi H, Inada K, Tsukamoto T, Kato T, Fukushima M, Inoue M, et al. (1998) A new prognostic factor for colorectal carcinoma, thymidylate synthase, and its therapeutic significance. Cancer 82:70-77
    • (1998) Cancer , vol.82 , pp. 70-77
    • Yamachika, T.1    Nakanishi, H.2    Inada, K.3    Tsukamoto, T.4    Kato, T.5    Fukushima, M.6    Inoue, M.7


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