Nucleolar function and size in cancer cells

American Journal of Pathology

Volumn 152, Issue 5, 1998, Pages 1291-1297

  Derenzini, Massimo ^a,d   Trerè, Davide ^a   Pession, Annalisa ^a   Montanaro, Lorenzo ^a   Sirri, Valentina ^b   Ochs, Robert L ^c  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b INSTITUT JACQUES MONOD   (France)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

^d UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE; DNA TOPOISOMERASE; FIBRILLARIN; MONOCLONAL ANTIBODY; PROTEIN; RIBOSOME RNA; TOLONIUM CHLORIDE;

ARTICLE; CANCER CELL; CHROMOSOME NOR; DENSITOMETRY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMMUNOBLOTTING; MORPHOMETRICS; NUCLEOLUS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

BLOTTING, WESTERN; CELL DIVISION; CELL NUCLEOLUS; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA TOPOISOMERASES, TYPE I; DNA, NEOPLASM; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; HUMANS; NEOPLASMS; NUCLEAR PROTEINS; POL1 TRANSCRIPTION INITIATION COMPLEX PROTEINS; RNA POLYMERASE I; RNA, NEOPLASM; RNA, RIBOSOMAL; TRANSCRIPTION FACTORS; TUMOR CELLS, CULTURED;

EID: 0031946191     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (42)

1. Hadjiolov AA: The nucleolus and ribosome biogenesis. Cell Biology Monographs. Wien, New York, Springer, 1995, pp 1-267
2. Busch H, Smetana K: Nucleoli of tumor cells. The Nucleolus. Edited by Busch H, Smetana K. New York, London, Academic Press, 1970, pp 448-471
3. Pianese G: Beitrag zur Histologie und Aetiologie der Carcinoma. Histologische und experimentelle Untersuchungen. Beitr Pathol Anat Allgem Pathol 1896, 142(suppl 1):1-193
4. Howell WM: Selective staining of nucleolus organizer regions (NORs). The Cell Nucleus. Edited by Busch H, Rothblum L. New York, Academic Press, 1982, pp 89-112
5. Olson MOJ: The role of proteins in nucleolar structure and function. The Eukaryotic Nucleolus: Molecular Biochemistry and Macromolecular Assemblies. Edited by Strauss PR, Wilson SH. Cadwell, New York, Telford Press Inc, 1990, pp 519-559
6. Pession A, Farabegoli F, Trerè D, Novelle F, Montanaro L, Sperti S, Rambelli F, Derenzini M: The Ag-NOR proteins and transcription and duplication of ribosomal genes in mammalian cell nuclei. Chromosoma 1991, 100:242-250
7. Derenzini M, Sirri V, Pession A, Trerè D, Roussel P, Ochs RL, Hernandez-Verdun D: Quantitative changes of the two major AgNOR proteins, nucleolin and protein B23, are related to stimulation of rDNA transcription. Exp Cell Res 1995, 219:276-282
8. Sirri V, Roussel P, Gendron MC, Hernandez-Verdun D: Amount of the two major Ag-NOR proteins, nucleolin, and protein B23 is cell-cycle dependent. Cytometry 1997, 28:147-156
9. Trerè D, Pession A, Derenzini M: The silver-stained proteins of interphasic nucleolar organizer regions as a parameter of cell duplication rate. Exp Cell Res 1989, 184:131-137
10. Derenzini M, Pession A, Trerè D: The quantity of nucleolar silverstained proteins is related to proliferating activity in cancer cells. Lab Invest 1990, 63:137-140
11. Öfner D, Hittmair A, Marth CR, Ofner C, Totsch M, Daxenbichler G, Mikuz G, Margreiter R, Schmid KW: Relationship between quantity of silver-stained nucleolar organizer regions associated proteins (Ag-NORs) and population doubling time in ten breast cancer cell lines. Pathol Res Pract 1992, 188:742-746
12. Derenzini M, Ploton D: Interphase nucleolar organizer regions. Molecular Biology in Histopathology. Edited by Crocker J. John Wiley and Sons Ltd, 1994, pp 231-249
13. Patterson MK Jr: Measurement of growth and viability of cells in culture. Methods in Enzymology. Edited by Jokoby WB, Pastran IH. New York, Academic Press, 1979, pp 141-167
14. Stirpe F, Novelle F: Effect on ionic strength, spermine and divalent ions with native and denatured deoxyribonucleic acid. Eur J Biochem 1970, 15:505-512
15. Roussel P, Belenguer P, Caldivell NJ, Amalric F, Hernandez-Verdun D: Nucleolin is an Ag-NOR protein, this property is determined by its amino-terminal domain independently of its phosphorylation state. Exp Cell Res 1992, 203:259-269
16. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685
17. Chan EKL, Imai H, Hamel JC, Tan EM: Human autoantibody to RNA polymerase I transcription factor hUBF: molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA upstream binding factor. J Exp Med 1991, 173:1239-1244
18. Reimer G, Raska I, Tan EM, Scheer YU: Human autoantibodies: probes for nucleolus structure and function. Virchow Arch B Cell Pathol 1987, 54:131-143
19. Bennion PJ, Horobin RW, Murgatroyd LB: The use of a basic dye (azure A or toluidine blue) plus a cationic surfactant for selective staining of RNA: a technical and molecular study. Stain Technol 1975, 50:307-313
20. Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet JJ: Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer regions at the optical level. Histochem J 1986, 18:5-14
21. O'Mahony DJ, Rothblum Ll: Identification of two groups of RNA polymerase I transcription factors UBF. Proc Natl Acad Sci USA 1991, 88:3180-3184
22. Liu LF, Miller KG: Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei. Proc Natl Acad Sci USA 1981, 78:3487-3491
23. Ochs RL, Lischwe MA, Spohn WH, Busch H: Fibrillarin, a new protein of the nucleolus identified by autoimmune sera. Biol Cell 1985, 54: 123-134
24. Moss T, Stefanovsky VY: Promotion and regulation of ribosomal transcription in eukaryotes by RNA polymerase I. Prog Nucleic Acid Res Mol Biol 1995, 50:25-66
25. Larson DE, Zahradka P, Sells BH: Control points in eucaryotic ribosome biogenesis. Biochem Cell Biol 1991, 69:5-22
26. Voit R, Kuhn A, Sander EE, Grummt I: Activation of mammalian ribosomal gene transcription requires phosphorylation of the nucleolar transcription factor UBF. Nucleic Acid Res 1995, 23:2593-2599
27. Glibetic M, Taylor L, Larson D, Hannan R, Sells B, Rothblum L: The RNA polymerase I transcription factor UBF is the product of a primary response gene. J Biol Chem 1995, 270:4209-4212
28. Lischwe MA, Ochs RL, Reddy R, Cook RG, Yeoman LC, Tan EM, Reichlin M, Busch H: Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pli, 8.5) rich in NG,NG-dimethylarginine. J Cell Biol 1985, 260:14304-14310
29. Kass S, Tyc K, Steitz JA, Sollner-Webb B: The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing. Cell 1990, 60:897-908
30. Mehes G, Pajor L: Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells: a flow cytometric assay. Cell Prolif 1995, 28:329-336
31. Muller MT, Pfund WP, Metha VB, Trask DK: Eukaryotic type I topoisomerase is enriched in the nucleolus and catalytically active on ribomal RNA. EMBO J 1985, 4:1237-1243
32. Zhang H, Wang JC, Liu LF: Involvement of DNA topoisomerase I in transcription of human ribosomal RNA genes Proc Natl Acad Sci USA 1988, 85:1060-1064
33. Buckwalter CA, Lin AH, Tanizawa A, Pommier YG, Cheng YC, Kaufmann SH: RNA synthesis inhibitors alter the subnuclear distribution of DNA topoisomerase I. Cancer Res 1996, 56:1674-1681
34. Bharti AK, Olson MOJ, Kufe DW, Rubin EH: Identification of a nucleolin binding site in human topoisomerase I. J Biol Chem 1996, 271: 1993-1997
35. Derenzini M, Sirri V, Trerè D, Ochs R: The quantity of nucleolar proteins nucleolin and protein B23 is related to cell doubling time in human cancer cells. Lab Invest 1995, 73:1-6
36. Trerè D: Critical analysis of the methods commonly employed in the assessment of cell proliferation: advantages of the NOR silver-staining technique in routine cyto-histopathology. Anal Cell Pathol 1993, 6:829-836
37. Gamel JW, McLean IW: Computerized histopathological assessment of malignant potential: II. A practical method for predicting survival following enucleation for uveal melanoma. Cancer 1983, 52:1032-1038
38. Baak JPA: The relative prognostic significance of nucleolar morphometry in invasive ductal breast cancer. Histopathology 1985, 9:437-444
39. Helpap B: Nucleolar grading of breast cancer Virchows Arch A 1989, 415:501-508
40. van Diest PJ, Mouriquand J, Schipper NM, Baak JPA: Prognostic value of nucleolar morphometric variables in cytological breast cancer specimens. J Clin Pathol 1990, 36:189-297
41. Derenzini M, Farabegoli F, Trerè D: Relationship between interphase AgNOR distribution and nucleolar size in cancer cells. Histochem J 1992, 24:951-956
42. Öfner D, Bankflavi A, Riehemann K, Bier B, Böcker B, Schmid KW: Wet autoclave pretreatment improves the visualization of silver-stained nucleolar organizer-region-associated proteins in routinely formalin-fixed and paraffin-embedded tissues. Mod Pathol 1994, 7:946-950