ATP and UTP at low concentrations strongly inhibit bone formation by osteoblasts: A novel role for the P2Y2 receptor in bone remodeling

Journal of Cellular Biochemistry

Volumn 86, Issue 3, 2002, Pages 413-419

  Hoebertz, Astrid ^a,b   Mahendran, Siva ^a   Burnstock, Geoffrey ^a   Arnett, Timothy R ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b RESEARCH INSTITUTE OF MOLECULAR PATHOLOGY   (Austria)

Author keywords

ATP; Bone; Osteoblast; P2 receptors; P2Y2; UTP

Indexed keywords

ADENOSINE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; MESSENGER RNA; NUCLEOTIDE; PURINE P2Y RECEPTOR; URIDINE TRIPHOSPHATE;

ANIMAL CELL; ARTICLE; BONE METABOLISM; BONE REMODELING; CALVARIA; CELL FUNCTION; CELL STIMULATION; NONHUMAN; OSSIFICATION; OSTEOBLAST; OSTEOCLAST; OSTEOLYSIS; PRIORITY JOURNAL; RAT; SIGNAL TRANSDUCTION;

ADENOSINE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANIMALS; BONE MARROW CELLS; DOSE-RESPONSE RELATIONSHIP, DRUG; OSTEOBLASTS; OSTEOGENESIS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, PURINERGIC P2; STIMULATION, CHEMICAL; URIDINE TRIPHOSPHATE;

RODENTIA;

EID: 0036935593     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcb.10236     Document Type: Article

References (35)

1. Bodin P, Burnstock G. 2001. Purinergic signalling: ATP release. Neurochem Res 26:959-969.
2. Bowler WB, Birch MA, Gallagher JA, Bilbe G. 1995. Identification and cloning of human P2U purinoceptor present in osteoclastoma, bone, and osteoblasts. J Bone Miner Res 10:1137-1145.
3. 2 receptors are expressed by human osteoclasts of giant cell tumor but do not mediate ATP-induced bone resorption. Bone 22:195-200.
4. Bowler WB, Tattersall JA, Hussein R, Dixon CJ, Cobbold PH, Gallagher JA. 1998b. Release of ATP by osteoblasts: Modulation by fluid shear forces. Bone 22:3S (abstract).
5. Bowler WB, Dixon CJ, Halleux C, Maier R, Bilbe G, Fraser WD, Gallagher JA, Hipskind RA. 1999. Signaling in human osteoblasts by extracellular nucleotides. J Biol Chem 274:14315-14324.
6. Bowler WB, Buckley KA, Gartland A, Hipskind RA, Bilbe G, Gallagher JA. 2001. Extracellular nucleotide signaling: A mechanism for integrating local and systemic responses in the activation of bone remodeling. Bone 28:507-512.
7. i release in rat osteoblasts independently of Gq activation or cyclic monophosphate accumulation. A mechanism for localizing systemic responses in bone. J Biol Chem 276:9565-9571.
8. Dixon SJ, Sims SM. 2000. P2 purinergic receptors on osteoblasts and osteoclasts: Potential targets for drug development. Drug Dev Res 49:187-200.
9. Dixon CJ, Bowler WB, Walsh CA, Gallagher JA. 1997. Effects of extracellular nucleotides on single cells and populations of human osteoblasts: Contribution of cell heterogeneity to relative potencies. Br J Pharmacol 120:777-780.
10. Hoebertz A, Townsend-Nicholson A, Glass R, Burnstock G, Arnett TR. 2000. Expression of P2 receptors in bone and cultured bone cells. Bone 27:503-510.
11. 1 receptor on bone cells. FASEB J 15:1139-1148.
12. Hsu HH, Anderson HC. 1977. A simple and defined method to study calcification by isolated matrix vesicles. Effect of ATP and vesicle phosphatase. Biochim Biophys Acta 500:162-172.
13. Jones SJ, Gray C, Boyde A, Burnstock G. 1997. Purinergic transmitters inhibit bone formation by cultured osteoblasts. Bone 21:393-399.
14. 7 receptors. J Biol Chem 277:7574-7580.
15. 2+, but not cyclic adenosine 3′,5′-monophosphate response to parathyroid hormone in rat osteoblastic cells. Endocrinology 136:1674-1685.
16. King BF, Townsend-Nicholson A, Burnstock G. 1998. Metabotropic receptors for ATP and UTP: Exploring the correspondence between native and recombinant nucleotide receptors. Trends Pharmacol Sci 19:506-514.
17. Komarova SV, Ataullakhanov FI, Globus RK. 2000. Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblasts. Am J Physiol Cell Physiol 279:C1220-C1229.
18. Kumagai H, Sakamoto H, Guggino S, Filburn CR, Sacktor B. 1989. Neurotransmitter regulation of cytosolic calcium in osteoblast-like bone cells. Calcif Tissue Int 45:251-254.
19. Kumagai H, Sacktor B, Filburn CR. 1991. Purinergic regulation of cytosolic calcium and phosphoinositide metabolism in rat osteoblast-like osteosarcoma cells. J Bone Miner Res 6:697-708.
20. Lazarowski ER, Harden TK. 1999. Quantitation of extracellular UTP using a sensitive enzymatic assay. Br J Pharmacol 127:1272-1278.
21. Lazarowski ER, Boucher RC, Harden TK. 2000. Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular nucleotide concentrations. J Biol Chem 275:31061-31068.
22. Luo LC, Yu H, Ferrier J. 1997. Differential purinergic receptor signalling in osteoclasts and osteoblastic cells. Cell Signal 9:603-607.
23. 6 cDNAs and identification of a pseudogene: Comparison of P2Y receptor subtype expression in bone and brain tissues. Biochem Biophys Res Commun 240:298-302.
24. Morrison MS, Turin L, King BF, Burnstock G, Arnett TR. 1998. ATP is a potent stimulator of the activation and formation of rodent osteoclasts. J Physiol Lond 511:495-500.
25. Nakamura E, Uezono Y, Narusawa K, Shibuya I, Oishi Y, Tanaka M, Yanagihara N, Nakamura T, Izumi F. 2000. ATP activates DNA synthesis by acting on P2X receptors in human osteoblast-like MG-63 cells. Am J Physiol Cell Physiol 279:C510-C519.
26. Pedersen S, Pedersen SF, Nilius B, Lambert IH, Hoffmann EK. 1999. Mechanical stress induces release of ATP from Ehrlich ascites tumor cells. Biochim Biophys Acta 1416:271-284.
27. Ralevic V, Burnstock G. 1998. Receptors for purines & pyrimidines. Pharmacol Rev 50:413-492.
28. i in rat osteoblastic cells by interaction with two receptor subtypes. Am J Physiol 263:C1040-C1048.
29. Saiag B, Bodin P, Shacoori V, Catheline M, Rault B, Burnstock G. 1995. Uptake and flow-induced release of uridine nucleotides from isolated vascular endothelial cells. Endothelium 2:279-285.
30. Schoefl C, Cuthbertson KS, Walsh CA, Mayne C, Cobbold P, von zur Muhlen A, Hesch RD, Gallagher JA. 1992. Evidence for P2-purinoceptors on human osteoblast-like cells. J Bone Miner Res 7:485-491.
31. Shimegi S. 1996. ATP and adenosine act as a mitogen for osteoblast-like cells (MC3T3-E1). Calcif Tissue Int 58:109-113.
32. Sistare FD, Rosenzweig BA, Contrera JG, Jordan B. 1994. Separate P2T and P2U purinergic receptors with similar second messenger signaling pathways in UMR-106 osteoblasts. J Pharmacol Exp Ther 269:1049-1061.
33. Sistare FD, Rosenzweig BA, Contrera JG. 1995. P2 purinergic receptors potentiate parathyroid hormone receptor-mediated increases in intracellular calcium and inositol trisphosphate in UMR-106 rat osteoblasts. Endocrinology 136:4489-4497.
34. 2 is a potential mediator of extracellular ATP action in osteoblast-like cells. Cell Adhes Commun 1:113-118.
35. Yu H, Ferrier J. 1993. Osteoblast-like cells have a variable mixed population of purino/nucleotide receptors. FEBS Lett 328:209-214.