Verification of a novel NADH-binding motif: Combinatorial mutagenesis of three amino acids in the cofactor-binding pocket of Corynebacterium 2,5-diketo-D-gluconic acid reductase

Journal of Molecular Evolution

Volumn 55, Issue 6, 2002, Pages 623-631

  Banta, Scott ^a   Anderson, Stephen ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

2,5 Diketo D gluconic acid reductase; 2 Keto L gulonic acid; Aldo keto reductase; Cofactor specificity; Cofactor binding pocket; Combinatorial mutagenesis; NADH; NADPH

Indexed keywords

2,5 DIKETO DEXTRO GLUCONIC ACID REDUCTASE; ASCORBIC ACID; GLUCONIC ACID; MUTANT PROTEIN; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG; 2,5 DIKETO D GLUCONATE 5 REDUCTASE; 2,5-DIKETO-D-GLUCONATE 5-REDUCTASE; ALDEHYDE REDUCTASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; COMBINATORIAL CHEMISTRY; CORYNEBACTERIUM; ENZYME ACTIVITY; ENZYME BINDING; ENZYME ISOLATION; MUTAGENESIS; NONHUMAN; PROTEIN ANALYSIS; RANDOMIZATION; REDUCTION; SCREENING; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PROTEIN MOTIF;

ACTINOBACTERIA (CLASS); CORYNEBACTERIUM; UNCULTURED ACTINOMYCETE;

AMINO ACID MOTIFS; BASE SEQUENCE; BINDING SITES; CORYNEBACTERIUM; DNA PRIMERS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS; NAD; SUGAR ALCOHOL DEHYDROGENASES;

EID: 0036892773     PISSN: 00222844     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00239-002-2345-x     Document Type: Article

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