The fate of monocytes during 24 h of culture as revealed by flow cytometry and electron microscopy

Journal of Immunological Methods

Volumn 270, Issue 1, 2002, Pages 63-76

  Lund, Per Kr ^a   Namork, Ellen ^b   Brorson, Sverre Henning ^a   Westvik, Åse Brit ^a   Joø, Gun Britt ^a   Øvstebø, Reidun ^a   Kierulf, Peter ^a  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

^b NORWEGIAN INSTITUTE OF PUBLIC HEALTH   (Norway)

Author keywords

Apoptosis; Monocytes; Phagocytosis

Indexed keywords

ANTIBODY; BROXURIDINE; CAMPTOTHECIN; CD14 ANTIGEN; LIPOCORTIN 5; PROPIDIUM IODIDE;

APOPTOSIS; ARTICLE; CELL COUNT; CELL CULTURE; CELL DEATH; CELL FATE; CELL LABELING; CELL LOSS; CELL MEMBRANE; CHROMATIN CONDENSATION; CONTROLLED STUDY; DNA DETERMINATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MONOCYTE; PHAGOCYTOSIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SCANNING ELECTRON MICROSCOPY; THAWING; TRANSMISSION ELECTRON MICROSCOPY;

ANNEXIN A5; ANTIGENS, CD14; APOPTOSIS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; FLOW CYTOMETRY; HUMANS; MEMBRANE POTENTIALS; MICROSCOPY, ELECTRON; MITOCHONDRIA; MONOCYTES; TIME FACTORS;

EID: 0036887646     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00272-7     Document Type: Article

References (31)

1. Andreesen R., Picht J., Löhr G.W. Primary cultures of human blood-born macrophages grown on hydrophobic teflon membranes. J. Immunol. Methods. 56:1983;295.
2. Bøyum A. Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest. 21(Suppl. 97):1968;77.
3. Brandtzaeg P., Øvstebø R., Kierulf P. Quantitative detection of bacterial lipopolysaccarides in clinical specimens. Pollard A.J., Maiden M.C.J. Meningococcal Disease. 2000;427 Humana Press, Totowa, NJ, USA.
4. Brorson S.H., Skjorten F. Mechanism for antigen detection on deplasticized epoxy sections. Micron. 26:1995;301.
5. Bussing A., Vervecken W., Wagner M., Wagner B., Pfuller U., Schietzel M. Expression of mitochondrial Apo2.7 molecules and caspase-3 activation in human lymphocytes treated with the ribosome-inhibiting mistletoe lectins and the cell membrane permeabilizing viscotoxins. Cytometry. 37:1999;133.
6. Callahan M.K., Williamson P., Schlegel R.A. Surface expression of phosphatidylserine on macrophages is required for phagocytosis of apoptotic thymocytes. Cell Death Differ. 7:2000;645.
7. Cossarizza A., Baccarani-Contri M., Kalashnikova G., Franceschi C. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′- tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem. Biophys. Res. Commun. 197:1993;40.
8. Covey J.M., Jaxel C., Kohn K.W., Pommier Y. Protein-linked DNA strand breaks induced in mammalian cells by camptothecin, an inhibitor of topoisomerase I. Cancer Res. 49:1989;5016.
9. Del Bino G., Lassota P., Darzynkiewicz Z. The S-phase cytotoxicity of camptothecin. Exp. Cell Res. 193:1991;27.
10. Devitt A., Moffatt O.D., Raykundalia C., Capra J.D., Simmons D.L., Gregory C.D. Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature. 392:1998;505.
11. Fadok V.A., Bratton D.L., Konowal A., Freed P.W., Westcott J.Y., Henson P.M. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-β, PGE2, and PAF. J. Clin. Invest. 101:1998;890.
12. Heidenreich S., Schmidt M., August C., Cullen P., Rademaekers A., Pauels H.-G. Regulation of human monocyte apoptosis by the CD14 molecule. J. Immunol. 159:1997;3178.
13. Jacobson M.D., Weil M., Raff M.C. Programmed cell death in animal development. Cell. 88:1997;347.
14. Kerr J., Wyllie A., Currie A. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer. 26:1972;239.
15. King M.A., Radicchi-Mastroianni M.A., Wells J.V. There is substantial nuclear and cellular disintegration before detectable phosphatidylserine exposure during the camptothecin-induced apoptosis of HL-60 cells. Cytometry. 40:2000;10.
16. Koester S.K., Roth P., Mikulka W.R., Schlossman S.F., Zhang C., Bolton W.E. Monitoring early cellular responses in apoptosis is aided by the mitochondrial membrane protein-specific monoclonal antibody APO2.7. Cytometry. 29:1997;306.
17. Krahling S., Callahan M.K., Williamson P., Schlegel R.A. Exposure of phosphatidylserine is a general feature in the phagocytosis of apoptotic lymphocytes by macrophages. Cell Death Differ. 6:1999;183.
18. Kroemer G., Petit P., Zamzami N., Vayssiere J.L., Mignotte B. The biochemistry of programmed cell death. FASEB J. 9:1995;1277.
19. Lund P.K., Joo G.B., Westvik A.B., Ovstebo R., Kierulf P. Isolation of monocytes from whole blood by density gradient centrifugation and counter-current elutriation followed by cryopreservation: six years' experience. Scand. J. Clin. Lab. Invest. 60:2000;357.
20. Lund P.K., Westvik A.B., Joo G.B., Ovstebo R., Haug K.B., Kierulf P. Flow cytometric evaluation of apoptosis, necrosis and recovery when culturing monocytes. J. Immunol. Methods. 252:2001;45.
21. Namork E., Heier H.E. Silver enhancement of gold probes (5-40 nm): single and double labeling of antigenic sites on cell surfaces imaged with backscattered electrons. J. Electron Microsc. Tech. 11:1989;102.
22. Parnaik R., Raff M.C., Scholes J. Differences between the clearance of apoptotic cells by professional and non-professional phagocytes. Curr. Biol. 10:2000;857.
23. Pradhan D., Krahling S., Williamson P., Schlegel R.A. Multiple systems for recognition of apoptotic lymphocytes by macrophages. Mol. Biol. Cell. 8:1997;767.
24. Roos D., de Boer M. Purification and Cryopreservation of Phagocytes from Human Blood. 1986;225 Academic Press, New York.
25. 6(3) or rhodamine 123, is a reliable fluorescent probe to assess ΔΨ changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411:1997;77.
26. Sanchez-Alcazar J.A., Ault J.G., Khodjakov A., Schneider E. Increased mitochondrial cytochrome c levels and mitochondrial hyperpolarization precede camptothecin-induced apoptosis in Jurkat cells. Cell Death Differ. 7:2000;1090.
27. Schlegel R.A., Krahling S., Callahan M.K., Williamson P. CD14 is a component of multiple recognition systems used by macrophages to phagocytose apoptotic lymphocytes. Cell Death Differ. 6:1999;583.
28. Vermes I., Haanen C., Steffens-Nakken H., Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescin labelled Annexin V. J. Immunol. Methods. 184:1995;39.
29. Wharton S., Riley P. The suppression of endogenous protein degradation by fractions of foetal calf serum: dialysed serum is less able to suppress degradation in aged cells. Cell Biochem. Funct. 4:1986;189.
30. Wiegand U.K., Corbach S., Prescott A.R., Savill J., Spruce B.A. The trigger to cell death determines the efficiency with which dying cells are cleared by neighbours. Cell Death Differ. 8:2001;734.
31. Zhang C., Ao Z., Seth A., Schlossman S.F. A mitochondrial membrane protein defined by a novel monoclonal antibody is preferentially detected in apoptotic cells. J. Immunol. 157:1996;3980.