Mechanism of inhibition of RNA polymerase I transcription by DNA-dependent protein kinase

Biological Chemistry

Volumn 383, Issue 11, 2002, Pages 1683-1690

  Michaelidis, Theologos M ^a   Grummt, Ingrid ^b  

^a MAX PLANCK INSTITUTE OF PSYCHIATRY   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

DNA dependent protein kinase; Ku; Phosphorylation; RNA polymerase I; SL1

Indexed keywords

ADENOSINE TRIPHOSPHATE; DNA DEPENDENT PROTEIN KINASE; DNA DIRECTED RNA POLYMERASE; KU ANTIGEN; RIBOSOME DNA;

ARTICLE; BINDING COMPETITION; COMPLEX FORMATION; CONTROLLED STUDY; DNA TEMPLATE; ENZYME ACTIVE SITE; ENZYME INACTIVATION; ENZYME REPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; PROTEIN PHOSPHORYLATION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

ANTIGENS, NUCLEAR; BLOTTING, WESTERN; DNA; DNA HELICASES; DNA, RIBOSOMAL; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ENZYME INHIBITORS; HUMANS; PHOSPHORIC MONOESTER HYDROLASES; PHOSPHORYLATION; POL1 TRANSCRIPTION INITIATION COMPLEX PROTEINS; PROMOTER REGIONS (GENETICS); PROTEIN KINASES; RNA POLYMERASE I; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

EID: 0036868349     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2002.189     Document Type: Article

References (33)

1. Allalunis-Turner, M. J., Barron, G. M., Day, R. S. 3rd, Dobler, K. D., and Mirzayans, R. (1993). Isolation of two cell lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs. Radiat. Res. 134, 349-354.
2. Bliss, T. M., and Lane, D. P. (1997). Ku selectively transfers between DNA molecules with homologous ends. J. Biol. Chem. 272, 5765-5773.
3. Boubnov, N. V., and Weaver, D. T. (1995). Scid cells are deficient in Ku and RPA phosphorylation by the DNA-dependent protein kinase. Mol. Cell. Biol. 15, 5700-5706.
4. Brou, C., Chaudhary, S., Davidson, I., Lutz, Y, Wu, J., Egly, J. M., Tora, L., and Chambon, P. (1993). Distinct TFIID complexes mediate the effect of different transcriptional activators. EMBO J. 12, 489-499.
5. Calsou, P, Frit, P, Humbert, O., Muller, C., Chen, D. J., and Salles, B. (1999). The DNA-dependent protein kinase catalytic activity regulates DNA end processing by means of Ku entry into DNA. J. Biol. Chem. 274, 7848-7856.
6. Cao, Q. P., Pitt, S., Leszyk, J., and Baril, E. F. (1994). DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen. Biochemistry 33, 8548-8557.
7. Chan, D. W., and Lees-Miller, S. P. (1996). The DNA-dependent protein kinase is inactivated by autophosphorylation of the catalytic subunit. J. Biol. Chem. 271, 8936-8941.
8. Dvir, A., Peterson, S. R., Knuth, M. W., Lu, H., and Dynan, W. S. (1992). Ku autoantigen is the regulatory component of a template associated protein kinase that phosphorylates RNA polymerase II. Proc. Natl. Acad. Sci. USA 89, 11920-11924.
9. Featherstone, C., and Jackson, S. P. (1999). Ku, a DNA repair protein with multiple cellular functions? Mutat. Res. 434, 3-15.
10. Gottlieb, T. M., and Jackson, S. P. (1993). The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72, 131-142.
11. Grummt, I. (1999). Regulation of mammalian ribosomal gene transcription by RNA polymerase I. Progr. Nucleic Acid Res. Mol. Biol. 62, 109-154.
12. Gu, X.-Y., Weinfeld, M. A., and Povirk, L. F. (1998). Implication of DNA-dependent protein kinase in an early, essential, local phosphorylation event during end-joining of DNA double-strand breaks in vitro. Biochemistry 37, 9827-9835.
13. Hartley, K. O., Gell, D., Smith, G. C., Zhang, H., Divecha, N., Connelly, M. A., Admon, A., Lees-Miller, S. P., Anderson, C. W., and Jackson, S. P. (1995). DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol-3 kinase and the ataxia telangiectasia gene product. Cell 82, 849-856.
14. Heix, J., Zomerdijk, J. C., Ravanpay, A., Tjian, R., and Grummt, I. (1997). Cloning of murine RNA polymerase I-specific TAFs: conserved interactions between the four subunits of the species-specific transcription factor TIF-IB/SL1. Proc. Natl. Acad. Sci. USA 94, 1733-1738.
15. Heix, J., Vente, A., Voit, R., Budde, A., Michaelidis, T. M., and Grummt, I. (1998). Mitotic silencing of human rRNA synthesis: inactivation of the promoter selectivity factor SL1 by cdc2/cyclin B mediated phosphorylation. EMBO J. 17, 7373-7381.
16. Hoff, C. M., Ghosh, A. K., Prabhakar, B. S., and Jacob, S. T. (1994). Enhancer 1 binding factor, a Ku-related protein, is a positive regulator or RNA polymerase I transcription initiation. Proc. Natl. Acad. Sci. USA 91, 762-766.
17. Jackson, S. P. (1997). DNA-dependent protein kinase. Int. J. Biochem. Cell. Biol. 29, 935-938.
18. Jin, S., and Weaver, D. T. (1997). Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J. 16, 6874-6885.
19. Koike M., Awaji, T., Kataoka, M., Tsujimoto., G., Kartasova, T., Koike, A., and Shiomi, T. (1999). Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis. J. Cell Sci. 112, 4031-4039.
20. Konno-Sato, S., Wu, J. M., and Carter, T. H. (1993). Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK). Biochem. Mol. Biol. Int. 31, 113-124.
21. Kuhn, A., Stefanovsky, V., and Grummt, I. (1993). The nucleolar transcription activator UBF relieves Ku antigen-mediated repression of mouse ribosomal gene transcription. Nucleic Acids Res. 21, 2057-2063.
22. Kuhn, A., Gottlieb, T. M., Jackson, S. P., and Grummt, I. (1995). DNA-dependent protein kinase: a potent inhibitor of transcription by RNA polymerase I. Genes Dev. 9, 193-203.
23. Labhart, P. (1995). DNA-dependent protein kinase specifically represses promoter-directed transcription initiation by RNA polymerase I. Proc. Natl. Acad. Sci. USA 92, 2934-2938.
24. Labhart, P. (1996). Phosphorylation of the N-terminal domain of Xenopus TATA-box binding protein by DNA-dependent protein kinase depends on the C-terminal core domain. FEBS Lett. 386, 110-114.
25. Lees-Miller, S. P., Chen, Y. R., and Anderson, C. W. (1990). Human cells contain a DNA-activated protein kinase that phosphorylates simian virus 40 T antigen, mouse p53, and the human Ku autoantigen. Mol. Cell Biol. 10, 6472-6481.
26. Lees-Miller, S. P., Godbout, R., Chan, D. W., Weinfeld, M., Day III, R. S., Barron, G. M., and Allalunis-Turner, J. A. (1995). Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line. Science 267, 1183-1185.
27. Manley, J. L., Fire, A., Sharp, P. A., and Gefter, M. L. (1980). DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc. Natl. Acad. Sci. USA 77, 3855-3859.
28. Niu, H., and Jacob, S. T. (1994). Enhancer 1 binding factor (E1BF), a Ku-related protein, is a growth-regulated RNA polymerase I transcription factor: association of a repressor activity with purified E1BF from serum-deprived cells. Proc. Natl. Acad. Sci. USA 91, 9101-9105.
29. Ochem, A. E., Skopac, D., Costa, M., Rabillaud, T, Vuillard, L., Simoncsits, A., Giacca, M, and Falaschi, A. (1997). Functional properties of the separate subunits of human DNA helicase II/Ku autoantigen. J. Biol. Chem. 272, 29919-29926.
30. Schnapp, A., and Grummt, I. (1996). Purification, assay and properties of RNA polymerase I and class I-specific transcription factors. Methods Enzymol. 273, 346-359.
31. Tuteja, N., Tuteja, R., Ochem, A., Taneja, P., Huang, N. W., Simoncsits, A., Sucic, S., Rahman, K., Marusic, L., Chen, J. et al. (1994). Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen. EMBO J. 13, 4991-5001.
32. Woodard, R. L., Anderson, M. G., and Dynan, W. S. (1999). Nuclear extracts lacking DNA-dependent protein kinase are deficient in multiple round transcription. J. Biol. Chem. 274, 478-485.
33. Yaneva, M., and Jhiang, S. (1991). Expression of the Ku protein during cell proliferation. Biochim. Biophys. Acta 1090, 181-187.