Adenovirus vector-mediated delivery of the prodrug-converting enzyme carboxypeptidase G2 in a secreted or GPI-anchored form: High-level expression of this active conditional cytotoxic enzyme at the plasma membrane

Cancer Gene Therapy

Volumn 9, Issue 11, 2002, Pages 897-907

  Cowen, Rachel L ^a   Williams, Judith C ^a   Emery, Steve ^b   Blakey, David ^b   Darling, John L ^c   Lowenstein, Pedro R ^a,d   Castro, Maria G ^a,d  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b ASTRAZENECA   (United Kingdom)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Adenovirus; Carboxypeptidase G2; Chemosensitivity; GPI anchor; Suicide gene therapy

Indexed keywords

[4 [BIS(2 IODOETHYL)AMINO]PHENYLOXYCARBONYL]GLUTAMIC ACID; ADENOVIRUS VECTOR; ANTINEOPLASTIC AGENT; CARBOXYPEPTIDASE G2; GANCICLOVIR; GLYCOSYLPHOSPHATIDYLINOSITOL; LOMUSTINE; PRODRUG; THYMIDINE KINASE; UNCLASSIFIED DRUG; ZD 2767P;

ARTICLE; BYSTANDER EFFECT; CANCER; CELL DEATH; CELL KILLING; CELL MEMBRANE; CHEMOSENSITIVITY; DRUG ACTIVATION; DRUG DIFFUSION; DRUG SENSITIVITY; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME RELEASE; GENE THERAPY; GENETIC TRANSDUCTION; GLIOMA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SUICIDE GENE THERAPY; TUMOR CELL LINE; VIRAL GENE DELIVERY SYSTEM;

ADENOVIRIDAE; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, THY-1; BASE SEQUENCE; CELL MEMBRANE; CELL SURVIVAL; DNA PRIMERS; EXONS; GAMMA-GLUTAMYL HYDROLASE; GENETIC VECTORS; GLYCOSYLPHOSPHATIDYLINOSITOLS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PRODRUGS; RATS; RECOMBINANT FUSION PROTEINS; TRANSFECTION; TUMOR CELLS, CULTURED;

ADENOVIRIDAE;

EID: 0036840427     PISSN: 09291903     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cgt.7700514     Document Type: Article

References (25)

1. Aghi M, Hochberg F, Breakefield XO. Prodrug activation enzymes in cancer gene therapy. J Gene Med. 2000;2:148-164.
2. Napier MP, Sharma SK, Springer CJ, et al. Antibody-directed enzyme prodrug therapy: Efficacy and mechanism of action in colorectal carcinoma. Clin Cancer Res. 2000;6:765-772.
3. Crystal RG, Hirschowitz E, Lieberman M, et al. Phase I study of direct administration of a replication deficient adenovirus vector containing the E. Coli cytosine deaminase gene to metastatic colon carcinoma of the liver in association with the oral administration of the pro-drug 5-fluorocytosine. Hum Gene Ther. 1997;8:985-1001.
4. Eck SL, Alavi JB, Alavi A, et al. Treatment of advanced CNS malignancies with the recombinant adenovirus H5.010RSVTK: A phase I trial. Hum Gene Ther. 1996;7:1465-1482.
5. Herman JR, Adler HL, Aguilar-Cordova E, et al. In situ gene therapy for adenocarcinoma of the prostate: A phase I clinical trial. Hum Gene Ther. 1999;10:1239-1249.
6. Sterman DH, Treat J, Litzky LA, et al. Adenovirus-mediated herpes simplex virus thymidine kinase/ganciclovir gene therapy in patients with localized malignancy: Results of a phase I clinical trial in malignant mesothelioma. Hum Gene Ther. 1998;9:1083-1092.
7. Morris JC, Ramsey WJ, Wildner O, et al. A phase I study of intralesional administration of an adenovirus vector expressing the HSV-1 thymidine kinase gene (AdV.RSV-TK) in combination with escalating doses of ganciclovir in patients with cutaneous metastatic malignant melanoma. Hum Gene Ther. 2000;11:487-503.
8. Minton NP, Atkinson T, Bruton CJ, Sherwood RF. The complete nucleotide sequence of the Pseudomonas gene coding for carboxypeptidase G2. Gene. 1984;31:31-38.
9. Sherwood RF, Melton RG, Alwan SM, Hughes P. Purification and properties of carboxypeptidase G2 from Pseudomonas sp. strain RS - 16. Use of a novel triazine dye affinity method. Eur J Biochem. 1985;148:447-453.
10. Rowsell S, Pauptit RA, Tucker AD, et al. Crystal structure of carboxypeptidase G2, a bacterial enzyme with applications in cancer therapy. Structure. 1997;5:337-347.
11. Blakey DC, Burke PJ, Davies DH, et al. ZD2767, an improved system for antibody-directed enzyme prodrug therapy that results in tumor regressions in colorectal tumor xenografts. Cancer Res. 1996;56:3287-3292.
12. Marais R, Spooner RA, Stribbling SM, et al. A cell surface tethered enzyme improves efficiency in gene-directed enzyme prodrug therapy. Nat Biotechnol. 1997;15:1373-1377.
13. Spooner RA, Martin J, Friedlos F, et al. In suicide gene therapy, the site of subcellular localization of the activating enzyme is more important than the rate at which it activates prodrug. Cancer Gene Ther. 2000;7:1348-1356.
14. Stribbling SM, Friedlos F, Martin J, et al. Regressions of established breast carcinoma xenografts by carboxypeptidase G2 suicide gene therapy and the prodrug CMDA are due to a bystander effect. Hum Gene Ther, 2000;11:285-292.
15. Clissold PM. A cDNA construct of tissue inhibitor metalloproteinases (TIMP) linked to the last exon of Thy-1 confers glycophospholipid anchorage on this naturally secreted protein. Biochem J. 1992;281:129-136.
16. Lowenstein PR, Morrison EE, Bain D, et al. Use of recombinant vectors derived from herpes simplex virus 1 mutant tsK for short-term expression of transgenes encoding cytoplasmic and membrane anchored proteins in postmitotic polarized cortical neurons and glial cells in vitro. Neuroscience. 1994;60:1059-1077.
17. Brown O, Cowen RL, Preston CM, et al. Subcellular posttranscriptional targeting: Delivery of an intracellular protein to the extracellular leaflet of the plasma membrane using a glycosyl-phosphatidylinositol (GPI) membrane anchor in neurons and polarised epithelial cells. Gene Ther. 2000;7:1947-1953.
18. Southgate TD, Windeatt S, Smith-Arica J, et al. Transcriptional targeting to anterior pituitary lactotrophic cells using recombinant adenovirus vectors in vitro and in vivo in normal and estrogen/sulpiride-induced hyperplastic anterior pituitaries. Endocrinology. 2000;141:3493-3505.
19. Cotten M, Baker A, Saltik M, et al. Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus. Gene Ther. 1994;1:239-246.
20. Dion LD, Fang J, Garver RI. Supernatant rescue assay in vivo polymerase chain reaction for detection of wild type adenovirus-contaminating recombinant adenovirus stocks. J Virol Methods. 1996;56:99-107.
21. Hooper M. Glycosyl-phosphatidylinositol anchored membrane enzymes. Clin Chim Acta. 1997;266:3-12.
22. Skehan P, Storeng R, Scudiero D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990;82:1107-1112.
23. Windeatt S, Southgate TD, Dewey RA, et al. Adenovirus-mediated herpes simplex virus type-1 thymidine kinase gene therapy suppresses oestrogen-induced pituitary prolactinomas. J Clin Endocrinol Metab. 2000;85:1296-1305.
24. Denny WA, Wilson WR. Bioreducible mustards: A paradigm for hypoxia-selective prodrugs of diffusible cytotoxins (HPDCs). Cancer Metastasis Rev. 1993;12:135-151.
25. Maleniak TC, Darling JL, Lowenstein PR, Castro MG. Comparison of chemosensitivity with HSV1-thymidine kinase/ganciclovir or murine fas ligand gene therapy strategies expressed by recombinant adenoviral vectors in short term human glioma cell lines derived from surgical biopsies. Cancer Gene Ther. 2001;8:589-598.