Analytical validation of a real-time reverse transcription-polymerase chain reaction quantitation of different transcripts of the Wilms' tumor suppressor gene (WT1)

Analytical Biochemistry

Volumn 309, Issue 1, 2002, Pages 127-136

  Lanvers, Claudia ^a   Paulo Vieira Pinheiro, Joao ^a   Hempel, Georg ^a   Wuerthwein, Gudrun ^a   Boos, Joachim ^a  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

Author keywords

Real rime RT PCR; WT1

Indexed keywords

CELL CULTURE; CLONE CELLS; DISEASES; RNA; TRANSCRIPTION; TUMORS;

ANALYTICAL VALIDATION; BREAST CANCER CELLS; CANCER DEVELOPMENT; HUMAN PROSTATE CANCER; LINEAR DYNAMIC RANGES; REAL-RIME RT-PCR; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; TUMOR SUPPRESSOR GENES;

POLYMERASE CHAIN REACTION;

ALTERNATIVE RNA SPLICING; ANALYTIC METHOD; ARTICLE; HUMAN; HUMAN CELL; MALIGNANT TRANSFORMATION; MOLECULAR CLONING; NEPHROBLASTOMA; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STRUCTURAL GENE; TRANSCRIPTION REGULATION; TUMOR SUPPRESSOR GENE; VALIDATION PROCESS; BIOSYNTHESIS; CALIBRATION; CELL CULTURE; CHEMISTRY; EXON; GENETICS; INTRON; MALE; METABOLISM; METHODOLOGY; MIDDLE AGED; OLIGONUCLEOTIDE PROBE; PROSTATE TUMOR; REPRODUCIBILITY; STANDARD;

FLUORESCEIN; FLUORESCENT DYE; MESSENGER RNA; PRIMER DNA; RNA; WT1 PROTEIN;

CALIBRATION; DNA PRIMERS; EXONS; FLUORESCEIN; FLUORESCENT DYES; GENES, WILMS TUMOR; HUMANS; INTRONS; MALE; MIDDLE AGED; OLIGONUCLEOTIDE PROBES; PROSTATIC NEOPLASMS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; TUMOR CELLS, CULTURED; WT1 PROTEINS;

EID: 0036807004     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00265-8     Document Type: Article

References (33)

1. D.L. Black, Protein diversity from alternative splicing: A challenge for bioinformatics and post-genome biology, Cell 103 (2000) 367-370.
2. A. Aigner, H. Juhl, C. Malerczyk, A. Tkybusch, C.C. Benz, F. Czubayko, Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation, Oncogene 20 (2001) 2101-2111.
3. A. Evdokiou, G.J. Atkins, S. Bouralexis, S. Hay, L.J. Raggatt, P.A. Cowled, S.E. Graves, M. Clayer, D.M. Findlay, Expression of alternatively-spliced MDM2 transcripts in giant cell tumours of bone, Int. J. Oncol. 19 (2001) 625-632.
4. M. Mixon, F. Kittrell, D. Medina, Expression of Brcal and splice variant Brca1delta11 RNA levels in mouse mammary gland during normal development and tumorigenesis, Oncogene 19 (2000) 5237-5243.
5. C. Dechsukhum, J.L. Ware, A. Ferreira-Gonzalez, D.S. Wilkinson, C.T. Garrett, Detection of a novel truncated WT1 transcript in human neoplasia, Mol. Diagn. 5 (2000) 117-128.
6. V.L. Bae, C.K. Jackson-Cook, A.R. Brothman, S.J. Maygarden, J.L. Ware, Tumorigenicity of SV40 T antigen immortalized human prostate epithelial cells: Association with decreased epidermal growth factor receptor (EGFR) expression, Int. J. Cancer 58 (1994) 721-729.
7. C. Jackson-Cook, V. Bae, W. Edelman, A. Brothman, J. Ware, Cytogenetic characterization of the human prostate epithelial cell line P69SV40T and its novel tumorigenic sublines M2182 and M15, Cancer. Genet. Cytogenet. 87 (1996) 14-23.
8. V.L. Bae, C.K. Jackson-Cook, S.J. Maygarden, S.R. Plymate, J. Chen, J.L. Ware, Metastatic sublines of an SV40 large T antigen immortalized human prostate epithelial cell line, Prostate 34 (1998) 275-282.
9. C. Englert, X. Hou, S. Maheswaran, P. Bennett, C. Ngwu, G.G. Re, A.J. Garvin, M.R. Rosner, D.A. Haber, WT1 suppresses synthesis of the epidermal growth factor receptor and induces apoptosis, EMBO J. 14 (1995) 4662-4675.
10. Z.Y. Wang, Q.Q. Qiu, K.T. Enger, T.F. Deuel, A second transcriptionally active DNA-binding site for the Wilms' tumor gene product, WT1, Proc. Natl. Acad. Sci. USA 90 (1993) 8896-8900.
11. I.A. Drummond, S.L. Madden, P. Rohwer-Nutter, G.I. Bell, V.P. Sukhatme, F.J. Rauscher III, Repression of the insulin-like growth factor II gene by the Wilms' tumor suppressor WT1, Science 257 (1992) 674-678.
12. K.E. Nichols, G.G. Re, A.J. Garvin, D.A. Haber, WT1 induces expression of insulin-like growth factor 2 in Wilms' tumor cells, Cancer Res. 55 (1995) 4540-4543.
13. H. Werner, C.T. Roberts Jr., D. LeRoith, The regulation of IGF-I receptor gene expression by positive and negative zinc-finger transcription factors, Adv. Exp. Med. Biol. 343 (1993) 91-103.
14. H. Werner, F.J. Rauscher III, V.P. Sukhatme, I.A. Drummond, C.T. Roberts Jr., D. LeRoith, The regulation of the insulin-like growth factor I receptor (IGF-I-R) gene by the tumor suppressor WT1 involves binding to sequences both upstream and down-stream of the IGF-I-R gene transcription start site, J. Biol. Chem. 269 (1994) 12577-12582.
15. R. Shimamura, G.C. Fraizer, J. Trapman, Y.F.C. Lau, G.F. Saunders, The Wilms' tumor gene WT1 can regulate genes involved in sex determination and differentiation: SRY, Mullerian-inhibiting substance, and the androgen receptor, Clin. Cancer Res. 3 (1997) 2571-2580.
16. M.A. English, J.D. Lich, Tumor-associated WT1 missense mutants indicate that transcriptional activation by WT1 is critical for growth control, J. Biol. Chem. 274 (1999) 13258-13263.
17. D.A. Haber, A.J. Buckler, WT1: A novel tumor suppressor gene inactivated in Wilms' tumor, New Biol. 4 (1992) 97-106.
18. K.W. Brown, J.E. Watson, V. Poirier, M.G. Mott, P.J. Berry, N.J. Maitland, Inactivation of the remaining allele of the wt1 gene in a Wilms' tumour from a WAGR patient, Oncogene 7 (1992) 763-768.
19. H.J. Im, G. Kong, H. Lee, Expression of Wilms tumor gene (WT1) in children with acute leukemia, Pediatr. Hematol. Oncol. 16 (1999) 109-118.
20. H. Tamaki, H. Ogawa, K. Ohyashiki, J.H. Ohyashiki, H. Iwama, K. Inoue, T. Soma, Y. Oka, T. Tatekawa, Y. Oji, A. Tsuboi, E.H. Kim, M. Kawakami, K. Fuchigami, M. Tomonaga, K. Toyama, K. Aozasa, T. Kishimoto, H. Sugiyama, The Wilms' tumor gene WT1 is a good marker for diagnosis of disease progression of myelodysplastic syndromes, Leukemia 13 (1999) 393-399.
21. H. Sugiyama, Wilms tumor gene (WT1) as a new marker for the detection of minimal residual disease in leukemia, Leuk. Lymphoma. 30 (1998) 55-61.
22. + progenitor cells by cytotoxic T lymphocytes specific for WT1, Blood 95 (2000) 2198-2203.
23. L. Bergmann, C. Miething, U. Maurer, J. Brieger, T. Karakas, E. Weidmann, D. Hoelzer, High levels of Wilms' tumor gene (wt1) mRNA in acute myeloid leukemias are associated with a worse long-term outcome, Blood 90 (1997) 1217-1225.
24. M. Hirose, The role of Wilms' tumor genes, J. Med. Invest. 46 (1999) 130-140.
25. K. Inoue, H. Sugiyama, H. Ogawa, M. Nakagawa, T. Yamagami, H. Miwa, K. Kita, A. Hiraoka, T. Masaoka, K. Nasu, WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia, Blood 84 (1994) 3071-3079.
26. C.A. Heid, J. Stevens, K.J. Livak, P.M. Williams, Real rime quantitative PCR, Genome Res. 6 (1996) 986-994.
27. U. Gibson, C.A. Heid, P.M. Williams, A novel method for real time quantitative RT-PCR, Genome Res. 6 (1996) 995-1001.
28. P. Holland, R. Abramson, R. Watson, D. Gelfand, Detection of specific polymerase chain reaction product by utilizing the 5′ → 3 ′ exonuclease activity of Thermus aquaticus DNA polymerase, Proc. Natl. Acad. Sci. USA 88 (1991) 7276-7280.
29. M.E. Kaighn, K.S. Narayan, Y. Ohnuki, J.B. Lechner, L.W. Jones, Establishment and characterization of a human prostate carcinoma cell line (PC-3), Invest. Urol. 17 (1979) 16-23.
30. W.H. Beyer, CRC Standard Mathematical Tables, 27th Ed, CRC Press, Boca Raton, FL, 1984.
31. K.J. Livak, L.J. McBride, Passive Internal References for the Detection of Nucleic Acid Amplification Products. United States Patent 5736333, 1998.
32. S.-C. Hung, R.A. Mathies, A.N. Glazer, Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations, Anal. Biochem. 255 (1998) 32-38.
33. C. Dechsukhum, Wilms' Tumor Suppressor Gene WT1 and Prostate Cancer. Ph.D. thesis at Virginia Commonwealth University, 1990, 31-50.