Substrate specificity of the metalloproteinase pregnancy-associated plasma protein-A (PAPP-A) assessed by mutagenesis and analysis of synthetic peptides: Substrate residues distant from the scissile bond are critical for proteolysis

Biochemical Journal

Volumn 367, Issue 1, 2002, Pages 31-40

  Laursen, Lisbeth S ^a   Overgaard, Michael T ^a   Nielsen, Claus G ^a   Boldt, Henning B ^a   Hopmann, Kathrin H ^a   Conover, Cheryl A ^a   Sottrup Jensen, Lars ^a   Giudice, Linda C ^a   Oxvig, Claus ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

Insulin like growth factor binding protein; Metzincin; Mitochondrial processing peptidase; Proteolysis

Indexed keywords

BIOASSAY; CARDIOVASCULAR SYSTEM; CELLS; INSULIN; IONIC STRENGTH; MUTAGENESIS;

PROTEOLYSIS;

ENZYMES;

METALLOPROTEINASE; PEPTIDASE; PREGNANCY ASSOCIATED PLASMA PROTEIN A; SOMATOMEDIN BINDING PROTEIN; ION; PEPTIDE; PROTEIN; SOMATOMEDIN BINDING PROTEIN 4; SOMATOMEDIN BINDING PROTEIN 5; ZINC;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL FREE SYSTEM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FIBROBLAST; MOLECULAR RECOGNITION; MUTAGENESIS; NONHUMAN; PEPTIDE ANALYSIS; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FAMILY; PROTEIN PROCESSING; RECEPTOR AFFINITY; AMINO ACID SEQUENCE; BINDING SITE; CELL LINE; CHEMISTRY; DOSE RESPONSE; GENETIC TRANSFECTION; HUMAN; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PH; PLASMID; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BINDING SITES; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; HYDROGEN-ION CONCENTRATION; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 4; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 5; IONS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDES; PLASMIDS; PREGNANCY-ASSOCIATED PLASMA PROTEIN-A; PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TRANSFECTION; ZINC;

EID: 0036798176     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20020831     Document Type: Article

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