Characterization of a pore-forming cytotoxin expressed by Salmonella enterica serovars Typhi and Paratyphi A

Infection and Immunity

Volumn 70, Issue 10, 2002, Pages 5759-5769

  Oscarsson, Jan ^a   Westermark, Marie ^a   Löfdahl, Sven ^a   Olsen, Björn ^a   Palmgren, Helena ^a   Mizunoe, Yoshimitsu ^a   Wai, Sun Nyunt ^a   Uhlin, Bernt Eric ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; CYTOLYSIN; CYTOLYSIN A; GENE PRODUCT; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; CELL FRACTIONATION; CONTROLLED STUDY; DNA SEQUENCE; GENE DELETION; GENE LOCUS; GENETIC COMPLEMENTATION; HEMOLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SALMONELLA ENTERICA; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SEROTYPE;

BASE SEQUENCE; CYTOTOXINS; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENES, BACTERIAL; HEMOLYSIN PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; PHENOTYPE; SALMONELLA PARATYPHI A; SALMONELLA TYPHI; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 0036786521     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.70.10.5759-5769.2002     Document Type: Article

References (56)

1. H-NS: A modulator of environmentally regulated gene expression
2. Escherichia coli hemolysin may damage target cell membranes by generating transmembrane pores
3. Pore-forming bacterial protein hemolysins (cytolysins)
4. SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages
5. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli
6. Salmonella spp. are cytotoxic for cultured macrophages
7. Role of Salmonella enterotoxin in overall virulence of the organism
8. The Escherichia coli K-12 sheA gene encodes a 34-kDa secreted hemolysin
9. Common themes in microbial pathogenicity revisited
10. Molecular genetic bases of Salmonella entry into host cells
11. Characteristics of the attenuated oral vaccine strain S. typhi Ty 21a
12. Isolation and characterization of Gal E mutant Ty 21a of Salmonella typhi: A candidate strain for a live, oral typhoid vaccine
13. The molecular basis for the differential regulation of the hlyE-encoded haemolysin of Escherichia coli by FNR and HlyX lies in the improved activating region 1 contact of HlyX
14. Cloned hemolysin genes from Escherichia coli that cause urinary tract infection determine different levels of toxicity in mice
15. Studies on transformation of Escherichia coli with plasmids
16. Interplay of global regulators and cell physiology in the general stress response of Escherichia coli
17. The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1
18. A galE via (Vi antigen-negative) mutant of Salmonella typhi Ty2 retains virulence in humans
19. Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli
20. Purification and partial characterization of the nephritis strain-associated protein from Streptococcus pyogenes, group A
21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
22. Cytocidal and apoptotic effects of the ClyA protein from Escherichia coli on primary and cultured monocytes and macrophages
23. A cytolysin encoded by Salmonella is required for survival within macrophages
24. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: Application to open reading frame characterization
25. Analysis of the SlyA-controlled expression, subcellular localization and pore-forming activity of a 34 kDa haemolysin (ClyA) from Escherichia coli K-12
26. SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli
27. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2
28. Genetic analysis of the interrelationship between plasmid replication and incompatibility
29. Salmonella typhimurium invasion induces apoptosis in infected macrophages
30. Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli
31. Protein toxins of Escherichia coli and Salmonella
32. Salmonella: A model for bacterial pathogenesis
33. Salmonella enteritidis in Antarctica: Zoonosis in man or humanosis in penguins?
34. Molecular analysis of the cytolytic protein ClyA (SheA) from Escherichia coli
35. Induction of haemolytic activity in Escherichia coli by the slyA gene product
36. The bacteriophage-associated Ehly1 and Ehly2 determinants from Escherichia coli O26:H - Strains do not encode enterohemolysins per se but cause release of the ClyA cytolysin
37. Expression of a pore-forming cytotoxin (ClyAHlyE, SheA) by typhoid Salmonella
38. Salmonella in sub-Antarctica: Low heterogeneity in Salmonella serotypes in South Georgian seals and birds
39. Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18
40. Altering the anaerobic transcription factor FNR confers a hemolytic phenotype on Escherichia coli K12
41. The live oral typhoid vaccine Ty21a is a rpoS mutant and is susceptible to various environmental stresses
42. Multiplication of Shigella flexneri within HeLa cells: Lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis
43. Molecular sieving by the Bacillus megaterium cell wall and protoplast
44. Interaction of Serratia marcescens hemolysin (ShlA) with artificial and erythrocyte membranes. Demonstration of the formation of aqueous multistate channels
45. 2+ oscillations in renal epithelial cells
46. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
47. E. coli hemolysin E (HlyEClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy
48. Pore-forming cytolysins of gram-negative bacteria
49. The synthesis and function of the Escherichia coli hemolysin and related RTX exotoxins
50. Silencing and activation of ClyA cytotoxin expression in Escherichia coli
51. Hydrogen peroxide formation and catalase activity in the lactic acid bacteria
52. Genetic locations of certain mutations conferring recombination deficiency in Escherichia coli
53. Identification of an essential virulence region on Salmonella plasmids
54. Characterization of the neisserial lipid-modified azurin bearing the H.8 epitope
55. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors