Cytocidal and apoptotic effects of the ClyA protein from Escherichia coli on primary and cultured monocytes and macrophages

Infection and Immunity

Volumn 68, Issue 7, 2000, Pages 4363-4367

  Lai, Xin He ^a,c   Arencibia, Ignacio ^a   Johansson, Anders ^a   Wai, Sun Nyunt ^a,b   Oscarsson, Jan ^a   Kalfas, Sotos ^a   Sundqvist, Karl Gösta ^a   Mizunoe, Yoshimitsu ^b   Sjöstedt, Anders ^a   Uhlin, Bernt Eric ^a  

^a UMEÅ UNIVERSITY   (Sweden)

^b KYUSHU UNIVERSITY   (Japan)

^c BEIJING HOSPITAL   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLYSIN;

ANIMAL CELL; ANTIGEN EXPRESSION; APOPTOSIS; ARTICLE; CELL KILLING; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXICITY; ESCHERICHIA COLI K 12; HEMOLYSIS; HUMAN; HUMAN CELL; MACROPHAGE; MONOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; APOPTOSIS; CELL DEATH; CELL LINE; DNA FRAGMENTATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEMOLYSIN PROTEINS; HUMANS; MACROPHAGES; MICE; MONOCYTES; U937 CELLS;

EID: 0033919438     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.7.4363-4367.2000     Document Type: Article

References (30)

1. Anderson, P. 1997. Kinase cascades regulating entry into apoptosis. Microbiol. Mol. Biol. Rev. 61:33-46.
2. Borenfreud, E., and J. A. Puerener. 1985. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24:119-124.
3. Chen, Y. J., and A. Zychlinsky. 1994. Apoptosis induced by bacterial pathogens. Microb. Pathog. 17:203-212.
4. Cooper, J. A. 1987. Effects of cytochalasin and phalloidin on actin. J. Cell Biol. 105:1473-1478.
5. del Castillo, I., S. C. Leal, F. Moreno, and I. del Castillo. 1997. The Escherichia coli K-12 sheA gene encodes a 34-kDa secreted haemolysin. Mol. Microbiol. 25:107-115.
6. Fernandez-Prada, C., B. D. Tall, S. E. Elliott, D. L. Hoover, J. P. Nataro, and M. M. Venkatesan. 1998. Hemolysin-positive enteroaggregative and cell-detaching Escherichia coli strains cause oncosis of human monocyte-derived macrophages and apoptosis of murine J774 cells. Infect. Immun. 66:3918-3924.
7. Gavrieli, Y., Y. Sherman, and S. A. Ben-Sasson. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.
8. Gómez-Gómez, J. M., J. Blázquez, F. Baquero, and J. L. Martínez. 1996. hns mutant unveils the presence of a latent haemolytic activity in Escherichia coli K-12. Mol. Microbiol. 19:909-910.
9. Green, J., and M. L. Baldwin. 1997. The molecular basis for the differential regulation of the hlyE-encoded haemolysin of Escherichia coli by FNR and HlyX lies in the improved activating region 1 contact of HlyX. Microbiology 143:3785-3793.
10. Herrmann, M., H.-M. Lorenz, R. Voll, W. Woith, and J. R. Kalden. 1994. A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res. 22:5506-5507.
11. Holm, A., S. Kalfas, and S. E. Holm. 1993. Killing of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by human polymorphonuclear leukocytes in serum and saliva. Oral Microbiol. Immunol. 8:134-140.
12. Iwase, M., E. T. Lally, P. Berthold, H. M. Korchak, and N. S. Taichman. 1990. Effects of cations and osmotic protectants on cytolytic activity of Actinobacillus actinomycetemcomitans. Infect. Immun. 58:1782-1788.
13. Johansson, A., L. Hänström, and S. Kalfas. Inhibition of Actinobacillus actinomycetemcomitans leukotoxicity by bacterial proteases. Oral Microbiol. Immunol., in press.
14. Jonas, D., B. Schultheis, C. Klas, P. H. Krammer, and S. Bhakdi. 1993. Cytocidal effects of E. coli hemolysin on human T lymphocytes. Infect. Immun. 61:1715-1721.
15. Kerr, J. F. R., A. H. Wyllie, and A. R. Currie. 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26:239-257.
16. Korzeniewski, C., and D. M. Callewaert. 1983. An enzyme-release assay for natural cytotoxicity. J. Immunol. Methods 64:313-320.
17. Lai, X.-H., S.-Y. Wang, and B. E. Uhlin. 1999. Expression of cytotoxicity by potential pathogens in the standard Escherichia coli collection of reference (ECOR) strains. Microbiology 145:3295-3303.
18. Lai, X.-H., J.-G. Xu, S. Melgar, and B. E. Uhlin. 1999. An apoptotic response by J774 macrophage cells is common upon infection with diarrheagenic Escherichia coli. FEMS Microbiol. Lett. 172:29-34.
19. Libby, S. J., W. Goebel, A. Ludwig, N. Buchmeier, F. Bowe, F. C. Fang, D. G. Guiney, J. G. Songer, and F. Heffron. 1994. A cytolysin encoded by Salmonella is required for survival within macrophages. Proc. Natl. Acad. Sci. USA 91:489-493.
20. Ludwig, A., S. Bauer, R. Benz, B. Bergmann, and W. Goebel. 1999. Analysis of the SlyA-controlled expression, subcellular localization and pore-forming activity of a 34 kDa haemolysin (ClyA) from Escherichia coli K-12. Mol. Microbiol. 31:557-567.
21. Ludwig, A., C. Tengel, S. Bauer, A. Bubert, R. Benz, H. J. Mollenkopf, and W. Goebel. 1995. SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli. Mol. Gen. Genet. 249:474-484.
22. McConkey, D. J., and S. Orrenius. 1994. Signal transduction pathways to apoptosis. Trends Cell Biol. 4:370-374.
23. Ojcius, D. M., A. Zychlinsky, L. M. Zheng, and J. D.-E. Young. 1991. Ionophore-induced apoptosis: role of DNA fragmentation and calcium fluxes. Exp. Cell Res. 197:43-49.
24. Oscarsson, J., Y. Mizunoe, L. Li, X.-H. Lai, Å. Wieslander, and B. E. Uhlin. 1999. Molecular analysis of the cytolytic protein ClyA (SheA) from Escherichia coli. Mol. Microbiol. 32:1226-1238.
25. Oscarsson, J., Y. Mizunoe, B. E. Uhlin, and D. J. Haydon. 1996. Induction of haemolytic activity in Escherichia coli by the slyA gene product. Mol. Microbiol. 20:191-199.
26. Ralph, P., and I. Nakoinz. 1975. Phagocytosis and cytolysis by a macrophage tumor and its cloned cell line. Nature 257:393-394.
27. Sundström, C., and K. Nilsson. 1976. Establishment and characterization of a human histiocytic lymphoma cell line (U937). Int. J. Cancer 17:565-577.
28. Uhlin, B. E., and Y. Mizunoe. 1994. Expression of a novel contact-hemolytic activity by E. coli. J. Cell Biochem. 18A:71.
29. Wallace, A. J., T. J. Stillman, A. Atkins, S. J. Jamieson, P. A. Bullough, J. Green, and P. J. Artymiuk. 2000. E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy. Cell 100:265-276.
30. Wyllie, A. H. 1980. Glucocorticoid induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555-556.