Thirty-cycle temperature optimization of a closed-cycle capillary PCR machine

BioTechniques

Volumn 33, Issue 3, 2002, Pages 557-564

  Chiou, Jeffrey T ^a   Matsudaira, Paul T ^a   Ehrlich, Daniel J ^a  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANNEALING; ENZYMES; HEAT AFFECTED ZONE; LIGHT SCATTERING; OPTICAL SENSORS; OPTIMIZATION; PRESSURE EFFECTS; THERMAL EFFECTS;

PRESSURIZED FLOW;

THERMAL CYCLING;

TAQ POLYMERASE; BACTERIAL DNA; PRIMER DNA;

ARTICLE; COMPUTER; DENATURATION; INTERMETHOD COMPARISON; MACHINE; POLYMERASE CHAIN REACTION; PRESSURE; SENSOR; TEMPERATURE; BACTERIOPHAGE LAMBDA; COMPARATIVE STUDY; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; GENETICS; HEAT; INSTRUMENTATION; METHODOLOGY; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY;

BACTERIOPHAGE LAMBDA; DNA PRIMERS; DNA, BACTERIAL; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; HEAT; POLYMERASE CHAIN REACTION; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE; TEMPERATURE;

EID: 0036752213     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02333st06_11825a     Document Type: Article

References (20)

1. Bej, A.K. and M.H. Mahbubani. 1994. Thermostable DNA polymerases for in vitro DNA amplifications, p. 219-237. In H.G. Griffin and A.M. Griffin (Eds.), PCR Technology: Current Innovations. CRC Press, Boca Raton, FL.
2. Belgrader, P., W. Benett, D. Hadley, J. Richards, P. Stratton, R. Mariella, Jr., and F. Milanovich. 1999. PCR detection of bacteria in seven minutes. Science 284:449-450.
3. Cha, R.S. and W.G. Thilly. 1995. Specificity, efficiency, and fidelity of PCR, p. 37-51. In C.W. Dieffenbach and G S. Dveksler (Eds.), PCR Primer: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
4. Chiou, J., P. Matsudaira, A. Sonin and D. Ehrlich. 2001. A closed-cycle capillary polymerase chain reaction machine. Anal. Chem. 73:2018-2021.
5. Friedman, N.A. and D.R. Meldrum. 1998. Capillary tube resistive thermal cycling. Anal. Chem. 70:2997-3002.
6. Giordano, B.C., J. Ferrance, S. Swedberg, A.F.R. Hühmer, and J.P. Landers. 2001. Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds. Anal. Biochem. 291:124-132.
7. Hühmer, A.F.R. and J.P. Landers. 2000. Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes. Anal. Chem. 72:5507-5512.
8. Khandurina, J., T.E. McKnight, S.C. Jacobson, L.C. Waters, R.S. Foote, and J.M. Ramsey. 2000. Integrated system for rapid PCR-based DNA analysis in microfluidic devices. Anal. Chem. 72:2995-3000.
9. Kopp, M.U., A.J. de Mello, and A. Manz. 1998. Chemical amplification: Continuous-flow PCR on a chip. Science 280:1046-1048.
10. Lagally, E.T., I. Medintz, and R.A. Mathies. 2001. Single-molecule DNA amplification and analysis in an integrated microfluidic device. Anal. Chem. 73:565-570.
11. Lagally, E.T., P.C. Simpson, and R.A. Mathies. 2000. Monolithic integrated microfluidic DNA amplification and capillary electrophoresis analysis system. Sensors Actuators B 63:138-146.
12. Nakano, H., K. Matsuda, M. Yohda, T. Nagamune, I. Endo, and T. Yamane. 1994. High speed polymerase chain reaction in constant flow. Biosci. Biotechnol. Biochem. 58:349-352.
13. Wetmur, J.G. 1991. DNA probes: Applications of the principles of nucleic acid hybridization. Crit. Rev. Biochem. Mol. Biol. 26:227-259.
14. Wetmur, J.G. and N. Davidson. 1968. Kinetics of renaturation of DNA. J. Mol. Biol. 31:349-370.
15. Wilding, P., M.A. Shoffner, and L.J. Kricka. 1994. PCR in a silicon microstructure. Clin. Chem. 40:1815-1818.
16. Wittwer, C.T., G.C. Fillmore, and D.J. Garling. 1990. Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal. Biochem. 186:328-331.
17. Wittwer, C.T., G.C. Fillmore, and D.R. Hillyard. 1989. Automated polymerase chain reaction in capillary tubes with hot air. Nucleic Acids Res. 17:4353-4357.
18. Wittwer, C.T. and D.J. Garling. 1991. Rapid cycle DNA amplification: Time and temperature optimization. BioTechniques 10:76-83.
19. Wittwer, C.T., K.M. Ririe, R.V. Andrew, D.A. David, R.A. Gundry, and U.J. Balis. 1997. The LightCycler™: A microvolume multisample fluorimeter with rapid temperature control. BioTechniques 22:176-181.
20. Woolley, A.T., D. Hadley, P. Landre, A.J. deMello, R.A. Mathies, and M.A. Northrup. 1996. Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. Anal. Chem. 68:4081-4086.