Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds

Analytical Biochemistry

Volumn 291, Issue 1, 2001, Pages 124-132

  Giordano, B C ^a   Ferrance, J ^a   Swedberg, S ^d   Huhmer A F R ^c   Landers, J P ^a,b  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c Thermo Finnigan   (United States)

^d AGILENT TECHNOLOGIES   (United States)

Author keywords

Capillary electrophoresis; Microchips; PCR fragment; Polyethylene glycol; Polyimide; Polymerase chain reaction; Thermocouple; Thermocycling

Indexed keywords

ALIPHATIC COMPOUNDS; CAPILLARY ELECTROPHORESIS; MICROPROCESSOR CHIPS; POLYETHYLENE GLYCOLS; POLYETHYLENES; POLYIMIDES; TEMPERATURE CONTROL; THERMAL CYCLING; THERMOCOUPLES;

DIRECT CONTACT; DNA AMPLIFICATION; MICROCHIPS; MICROFABRICATED DEVICES; PCR FRAGMENT; PCR PRODUCTS; POLYMERIC MICROCHIPS; TAQ POLYMERASE;

POLYMERASE CHAIN REACTION;

BACTERIOPHAGE DNA; BUFFER; MACROGOL; NUCLEIC ACID; POLYIMIDE; POLYMER; TAQ POLYMERASE;

ACCURACY; ARTICLE; BACTERIOPHAGE LAMBDA; CAPILLARY ELECTROPHORESIS; DNA MICROARRAY; ENZYME INACTIVATION; GENE AMPLIFICATION; INFRARED RADIATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; THERMOREGULATION;

EID: 0035311755     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4974     Document Type: Article

References (30)

1. Molecular diagnostics on microfabricated electrophoretic devices: From slab gel- to capillary- to microchip-based assays of T- and B-cell lymphoproliferative disorders
2. Single-strand conformation polymorphism analysis by capillary and microchip electrophoresis: A fast, simple method for detection of common mutations in BRCA1 and MRCA2
3. Rapid detection of deletion, insertion, and substitution mutations via heteroduplex analysis using capillary- and microchip-based electrophoresis
4. Rapid diagnosis of herpes simplex encephalitis using microchip electrophoresis of PCR products
5. Degenerate oligonucleotide primed polymerase chain reaction and capillary electrophoretic analysis of human DNA on microchip based devices
6. Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis
7. Microfluidic assays of acetylcholinesterase inhibitors
8. High-speed DNA genotyping using microfabricated capillary array electrophoresis chips
9. Minimizing the time required for DNA amplification by efficient heat-transfer to small samples
10. Automated polymerase chain-reaction in capillary tubes with hot air
11. Fully automated DNA reaction and analysis in a fluidic capillary instrument
12. The LightCycler, a microvolume multisample fluorimeter with rapid temperature control
13. Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers
14. Chip PCR.1. Surface passivation of microfabricated silicon-glass chips for PCR
15. Chip PCR.2. Investigation of different PCR amplification systems in microfabricated silicon-glass chips
16. Multiple sample PCR amplification and electrophoretic analysis on a microchip
17. Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing
18. An integrated nanoliter DNA analysis device
19. Chemical amplification: Continuous-flow PCR on a chip
20. Monolithic integrated microfluidic DNA amplification and capillary electrophoresis analysis system
21. Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device
22. Real-time microchip PCR for detecting single-base differences in viral and human DNA
23. Integrated system for rapid PCR-based DNA analysis in microfluidic devices
24. Analysis of DNA fragments by microchip electrophoresis fabricated on poly(methylmethacrylate) substrates using a wire-imprinting method
25. Plastic microchip electrophoresis for analysis of PCR products of hepatitis C virus
26. Integrated capillary electrophoresis on flexible silicone microdevices: Analysis of DNA restriction fragments and detection of single DNA molecules on microchips
27. Plastic microchip electrophoresis with analyte velocity modulation. Application to fluorescence background rejection
28. Infrared-mediated thermocycling for ultrafast polymerase chain reaction amplification of DNA
29. Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes