On-line coupling isoelectric focusing with transient isotachophoresis-zone electrophoresis: A two-dimensional separation system for proteomics

Electrophoresis

Volumn 23, Issue 18, 2002, Pages 3160-3167

  Mohan, Deepa ^a   Lee, Cheng S ^a  

^a Bldg 142   (United States)

Author keywords

Capillary isoelectric focusing; Transient isotachophoresis; Zone electrophoresis

Indexed keywords

CARBON DIOXIDE; CARBONIC ANHYDRASE; ELECTROLYTES; ELECTROPHORETIC MOBILITY; ELECTROSPRAY IONIZATION; FOCUSING; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PH;

CAPILLARY ISOELECTRIC FOCUSING; ISO-ELECTRIC POINTS; ISOELECTRIC FOCUSING; ON-LINE COUPLING; PH GRADIENTS; PROTEOMICS; SEPARATION SYSTEMS; TRANSIENT ISOTACHOPHORESIS; TWO-DIMENSIONAL SEPARATIONS; ZONE ELECTROPHORESIS;

PEPTIDES;

AMPHOLYTE; CARBONATE DEHYDRATASE II; CYTOCHROME C; ELECTROLYTE; PEPTIDE; PROTEIN; RIBONUCLEASE A; PANCREATIC RIBONUCLEASE; PEPTIDE FRAGMENT; TRYPSIN;

ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; ISOTACHOPHORESIS; MASS SPECTROMETRY; NONHUMAN; ONLINE SYSTEM; PH; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEOMICS; SENSITIVITY ANALYSIS; SEPARATION TECHNIQUE; AMINO ACID SEQUENCE; ANIMAL; CAPILLARY ELECTROPHORESIS; CHEMISTRY; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY;

AMINO ACID SEQUENCE; AMPHOLYTE MIXTURES; ANIMALS; CARBONIC ANHYDRASE II; CYTOCHROME C GROUP; ELECTROPHORESIS, CAPILLARY; ISOELECTRIC FOCUSING; PEPTIDE FRAGMENTS; PROTEOMICS; RIBONUCLEASE, PANCREATIC; TRYPSIN;

EID: 0036726557     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2683(200209)23:18<3160::AID-ELPS3160>3.0.CO;2-L     Document Type: Article

References (34)

1. Persidis, A., Nature Biotech. 1998, 16, 393-394.
2. Hillenkamp, F., Karas, M., Beavis, R. C., Chait, B. T, Anal. Chem. 1991, 63, 1193A-1203A.
3. Fenselau, C., Anal. Chem. 1997, 69, 661A-665A.
4. Kebarle, P., Tang, L., Anal. Chem. 1993, 65, 972A-986A.
5. Yates, J. R. III, J. Mass Spectrom. 1998, 33, 1-19.
6. Klose, J., Kobalz, U., Electrophoresis 1995, 16, 1034-1059.
7. Jungblut, P., Thiede, B., Zimny-Arndt, U., Müller, E.-C., Scheler, C., Wittmann-Liebold, B., Otto, A., Electrophoresis 1996, 17, 839-847.
8. Rabilloud, T., Anal. Chem. 2000, 72, 48A-55A.
9. Wall, D. B., Kachman, M. T., Gong, S., Hinderer, R., Parus, S., Misek, D. E., Hanash, S. M., Lubman, D. M., Anal. Chem. 2000, 72, 1099-1111.
10. Link, A. J., Eng, J., Schieltz, D. M., Carmack, E., Mize, G. J., Morris, D. R., Garvik, B. M., Yates, J. R. III, Nature Biotech. 1999, 17, 676-682.
11. Washburn, M. P., Wolters, D., Yates, J. R. III, Nature Biotech. 2001, 19, 242-247.
12. Opiteck, G. J., Lewis, K. C., Jorgenson, J. W., Anderegg, R. J., Anal. Chem. 1997, 69, 1518-1524.
13. Opiteck, G. J., Jorgenson, J. W., Anderegg, R. J., Anal. Chem. 1997, 69, 2283-2291.
14. Hooker, T F., Jorgenson, J. W., Anal. Chem. 1997, 69, 4134-4142.
15. Lewis, K. C., Opiteck, G. J., Jorgenson, J. W., Sheely, D., J. Am. Soc. Mass Spectrom. 1997, 8, 495-500.
16. Rocklin, R. D., Ramsey, R. S., Ramsey, J. M., Anal. Chem. 2000, 72, 5244-5249.
17. Gottschlich, N., Jacobson, S. C., Culbertson, C. T, Ramsey, J. M., Anal. Chem. 2001, 73, 2669-2674.
18. Becker, H., Lowack, K., Manz, A., J. Micromech. Microeng. 1998, 8, 24-28.
19. Conti, M., Gelfi, C., Righetti, P. G., Electrophoresis 1996, 17, 1485-1491.
20. Conti, M., Galassi, M., Bossi, A., Righetti, R G., J. Chromatogr. A 1997, 757, 237-245.
21. Shen, Y., Smith, R. D., J. Microcol. Sep. 2000, 12, 135-141.
22. Shen, Y., Berger, S. J., Anderson, G. A., Smith, R. D., Anal. Chem. 2000, 72, 2154-2159.
23. Severs, J. C., Smith, R. D., Anal. Chem. 1997, 69, 2154-2158.
24. Yang, L., Lee, C. S., Hofstadler, S. A., Smith, R. D., Anal. Chem. 1998, 70, 4945-4950.
25. Giddings, J. C., Unified Separation Science, John Wiley and Sons, New York 1991, pp. 112-114.
26. Hille, J. M., Freed, J. M., Wätzig, H., Electrophoresis 2001, 22, 4035-4052.
27. Mikkers, F. E. P., Everaerts, F. M., Verheggen, T P. E., J. Chromatogr. 1979, 169, 1-10.
28. Foret, F., Scoko, E., Karger, B. L., Electrophoresis 1993, 14, 417-428.
29. Thompson, T J., Foret, F., Vouros, P., Karger, B. L., Anal. Chem. 1993, 65, 900-906.
30. Conrads, T P., Anderson, G. A., Veenstra, T D., Pasa-Tolic, L., Smith, R. D., Anal Chem. 2000, 72, 3349-3354.
31. Lamoree, M. H., Tjaden, U. R., van der Greef, J., J. Chromatogr. A 1997, 777, 31-39.
32. Wolters, D. A., Washburn, M. P., Yates, J. R. III, Anal. Chem. 2001, 73, 5683-5690.
33. Rodriguez-Diaz, R., Wehr, T., Zhu, M., Levi, V., in: Landers, J. P. (Ed), Handbook of Capillary Electrophoresis, CRC Press, Boca Raton, FL 1997, pp. 101-138.
34. Righetti, R G., Castagna, A., Herbert, B., Anal. Chem. 2001, 73, 320A-326A.