Cleavage at the furin consensus sequence RAR/KR109 and presence of the intervening peptide of the respiratory syncytial virus fusion protein are dispensable for virus replication in cell culture

Journal of Virology

Volumn 76, Issue 18, 2002, Pages 9218-9224

  Zimmer, Gert ^a   Conzelmann, Karl Klaus ^a   Herrler, Georg ^a  

^a UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FURIN; HYBRID PROTEIN; TRYPSIN; VIRUS GLYCOPROTEIN; VIRUS RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOPATHOGENIC EFFECT; DELETION MUTANT; DISULFIDE BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; RADIOIMMUNOPRECIPITATION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VERO CELL; VIRUS CULTURE; VIRUS INFECTIVITY; VIRUS RECOMBINANT; VIRUS REPLICATION; VIRUS TITRATION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CELL LINE; CERCOPITHECUS AETHIOPS; CONSENSUS SEQUENCE; CYTOPATHOGENIC EFFECT, VIRAL; FURIN; GENE DELETION; MOLECULAR SEQUENCE DATA; PEPTIDES; RESPIRATORY SYNCYTIAL VIRUS, BOVINE; SUBTILISINS; VERO CELLS; VIRAL FUSION PROTEINS; VIRUS REPLICATION;

EID: 0036721195     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.18.9218-9224.2002     Document Type: Article

References (41)

1. Human and bovine respiratory syncytial virus: Immuno-pathologic mechanisms
2. Seroepizootiologic study of bovine respiratory syncytial virus in a dairy herd
3. Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter
4. Recombinant respiratory syncytial virus from which the entire SH gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse
5. Neurokinin mediation of edema and inflammation
6. Structure of the hemagglutinin precursor cleavage site, a determinant of influenza pathogenicity and the origin of the labile conformation
7. Respiratory syncytial virus
8. Demonstration of two distinct cytopathic effects with syncytium formation-defective human immunodeficiency virus type 1 mutants
9. Paramyxovirus fusion (F) protein: A conformational change on cleavage activation
10. Identification of a linear heparin binding domain for human respiratory syncytial virus attachment glycoprotein G
11. The fusion glycoprotein of human respiratory syncytial virus facilitates virus attachment and infectivity via an interaction with cellular heparan sulfate
12. Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion
13. Glycosaminoglycan sulfation requirements for respiratory syncytial virus infection
14. Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection
15. Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160
16. Analysis of respiratory syncytial virus F, G, and SH proteins in cell fusion
17. Airway inflammation and tachykinins: Prospects for the development of tachykinin receptor antagonists
18. Recombinant bovine respiratory syncytial virus with deletions of the G or SH genes: G and F proteins bind heparin
19. Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: Clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant
20. Immunity to human and bovine respiratory syncytial virus
21. Host cell proteases controlling viral pathogenicity
22. Activation of viral spike proteins by host proteases
23. Heparin-dependent attachment of respiratory syncytial virus (RSV) to host cells
24. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
25. Peripheral actions of tachykinins
26. Recombinant measles virus requiring an exogenous protease for activation of infectivity
27. Binding of human respiratory syncytial virus to cells: Implication of sulfated cell surface proteoglycans
28. Reverse genetics demonstrates that proteolytic processing of the ebola virus glycoprotein is not essential for replication in cell culture
29. Analysis of bovine respiratory syncytial virus envelope glycoproteins in cell fusion
30. Motor control of airway goblet cells and glands
31. Respiratory syncytial virus fusion protein mediates inhibition of mitogen-induced T-cell proliferation by contact
32. Antibodies to CD9, a tetraspan transmembrane protein, inhibit canine distemper virus-induced cell-cell fusion but not virus-cell fusion
33. Respiratory syncytial virus. Brief review
34. Proteolytic processing of human cytomegalovirus glycoprotein B is dispensable for viral growth in culture
35. Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein gene
36. Tachykinin-like peptides and their receptors: A review
37. The hemagglutinin of canine distemper virus determines tropism and cyto-pathogenicity
38. Proteolytic cleavage of the fusion protein but not membrane fusion is required for measles virus-induced immunosuppression in vitro
39. Recombinant respiratory syncytial virus bearing a deletion of either the NS2 or SH gene is attenuated in chimpanzees
40. N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus
41. Proteolytic activation of respiratory syncytial virus fusion protein: Cleavage at two furin consensus sequences