Modular engineering of a Group I intron ribozyme

Nucleic Acids Research

Volumn 30, Issue 15, 2002, Pages 3473-3480

  Ohuchi, Shoji J ^a   Ikawa, Yoshiya ^a   Shiraishi, Hideaki ^a   Inoue, Tan ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOZYME; RNA;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SUBSTRATE; IN VITRO STUDY; INTRON; MOLECULAR CLONING; MOLECULAR LIBRARY; PRIORITY JOURNAL; PROTEIN DOMAIN; WILD TYPE;

EID: 0036682095     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkf453     Document Type: Article

References (36)

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2. The structure of group I ribozymes
3. Minimum secondary structure requirements for catalytic activity of a self-splicing group I intron
4. Building a catalytic active site using only RNA
5. New loop-loop tertiary interactions in self-splicing introns of subgroup IC and ID: A complete 3D model of the Tetrahymena thermophila ribozyme
6. Modeling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis
7. A preorganized active site in the crystal structure of the Tetrahymena ribozyme
8. A hydrogen-bonding triad stabilizes the chemical transition state of a group I ribozyme
9. Identification of phosphate groups important to self-splicing of the Tetrahymena rRNA intron as determined by phosphorothioate substitution
10. Analysis of the role of phosphate oxygens in the group I intron from Tetrahymena
11. Metal coordination sites that contribute to structure and catalysis in the group I intron from Tetrahymena
12. The environment of two metal ions surrounding the splice site of a group I intron
13. Visualizing metal-ion-binding sites in group I introns by iron(II)-mediated Fenton reactions
14. The chemical basis of adenosine conservation throughout the Tetrahymena ribozyme
15. Localization of metal ion binding sites in Group I intron RNA
16. Identification of an active site ligand for a Group I ribozyme catalytic metal ion
17. Minimal catalytic domain of a group I self-splicing intron RNA
18. Self-splicing of the Tetrahymena group I ribozyme without conserved base-triples
19. Domain structure of the ribozyme from eubacterial ribonuclease P
20. Design and isolation of ribozyme-substrate pairs using RNase P-based ribozymes containing altered substrate binding sites
21. Ribonuclease P: Unity and diversity in a tRNA processing ribozyme
22. Ribonuclease P
23. Selection of a ribozyme that functions as a superior template in a self-copying reaction
24. In vitro genetic analysis of the Tetrahymena self-splicing intron
25. In vitro recombination and mutagenesis by overlap extension PCR
26. Enzymatic approaches to probing of RNA secondary and tertiary structure
27. Algorithms and thermodynamics for RNA secondary structure prediction: A practical guide
28. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure
29. Selection of novel forms of a functional domain within the Tetrahymena ribozyme
30. Characterization of the newly constructed domains that replace P5abc within the Tetrahymena ribozyme
31. Selection in vitro of novel ribozymes from a partially randomized U2 and U6 snRNA library
32. RNA-catalyzed RNA polymerization: Accurate and general RNA-templated primer extension
33. In vitro selection of nucleoprotein enzymes
34. Allosteric selection of ribozymes that respond to the second messengers cGMP and cAMP
35. Representation of the secondary and tertiary structure of group I introns
36. Genetic delineation of functional components of the group I intron in the phage T4 td gene