Cotransformation of Trichoderma harzianum with β-glucuronidase and green fluorescent protein genes provides a useful tool for monitoring fungal growth and activity in natural soils

Applied and Environmental Microbiology

Volumn 66, Issue 2, 2000, Pages 810-815

  Bae, Yeoung Seuk ^a   Knudsen, Guy R ^a  

^a UNIVERSITY OF IDAHO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLUCURONIDASE; GLUCURONIC ACID; GREEN FLUORESCENT PROTEIN; HYGROMYCIN B; MACROGOL;

FUNGUS; GROWTH; METHODOLOGY; MONITORING;

ARTICLE; CONTROLLED STUDY; FUNGUS CULTURE; FUNGUS HYPHAE; FUNGUS SPORE; GENE EXPRESSION; GENETIC TRANSFORMATION; GROWTH RATE; MARKER GENE; MICROBIAL GROWTH; MYCELIUM; NONHUMAN; SOIL; TRICHODERMA HARZIANUM;

ANTI-BACTERIAL AGENTS; ASCOMYCOTA; BLOTTING, SOUTHERN; DRUG RESISTANCE, MICROBIAL; FUNGI; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; HYGROMYCIN B; LUMINESCENT PROTEINS; PROTOPLASTS; SOIL MICROBIOLOGY; TRANSFORMATION, GENETIC; TRICHODERMA;

FUNGI; HYPOCREA LIXII; SCLEROTINIA SCLEROTIORUM; TRICHODERMA;

EID: 0033966761     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.2.810-815.2000     Document Type: Article

References (37)

1. Abd-El Moity, T. H., G. C. Papavizas, and M. N. Shatla. 1982. Induction of new isolates of Trichoderma harzianum tolerant to fungicides and their experimental use for control of white rot of onion. Phytopathology 72:396-400.
2. Ahmad, J. S., and R. Baker. 1987. Rhizosphere competence of Trichoderma harzianum. Phytopathology 77:182-189.
3. Bin, L., G. R. Knudsen, and D. J. Eschen. 1991. Influence of an antagonistic strain of Pseudomonas fluorescens on growth and ability of Trichoderma harzianum to colonize sclerotia of Sclerotinia sclerotiorum in soil. Phytopathology 81:994-1000.
4. Bollen, G. J., J. Middelkoop, and T. W. Hofman. 1991. Effects of soil fauna on infection of potato sprouts by Rhizoctonia solani, p. 27-34. In A. B. R. Beemster, G. J. Bollen, M. Gerlagh, M. A. Ruissen, B. Schippers, and A. Tempel (ed.), Biotic interactions and soil-borne diseases. Proceedings of the First Conference of the European Foundation for Plant Pathology. Elsevier Science Publishing Co., Inc., New York, N.Y.
5. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
6. Cheng, L., J. Fu, A. Tsukamoto, and R. G. Hawley. 1996. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells. Nat. Biotechnol. 14:606-609.
7. Chet, I. 1987. Trichoderma - application, mode of action, and potential as biocontrol agent of soilborne plant pathogenic fungi, p. 137-160. In I. Chet (ed.), Innovative approaches to plant disease control. John Wiley & Sons, Inc., New York, N.Y.
8. Chet, I., and R. Baker. 1981. Isolation and biocontrol potential of Trichoderma hamatum from soil naturally suppressive to Rhizoctonia solani. Phytopathology 71:286-290.
9. Cormack, B. P., G. Bertram, M. Egerton, N. A. R. Gow, S. Falkow, and A. J. P. Brown. 1997. Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans. J. Gen. Microbiol. 143:303-311.
10. Cubitt, A. B., R. Heim, S. R. Adams, A. E. Boyd, L. A. Gross, and R. Y. Tsien. 1995. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20:448-455.
11. Dandurand, L. M., and G. R. Knudsen. 1993. Influence of Pseudomonas fluorescens on hyphal growth and biocontrol activity of Trichoderma harzianum in the spermosphere and rhizosphere of pea. Phytopathology 83:265-270.
12. Elad, Y., I. Chet, and Y. Henis. 1981. Biological control of Rhizoctonia solani in strawberry fields by Trichoderma harzianum. Plant Soil 60:245-254.
13. Elad, Y., Y. Hadar, E. Hadar, I. Chet, and Y. Henis. 1981. Biological control of Rhizoctonia solani by Trichoderma harzianum in carnation. Plant Dis. 65:675-677.
14. Green, H., and D. F. Jensen. 1995. A tool for monitoring Trichoderma harzianum. II. The use of a GUS transformant for ecological studies in the rhizosphere. Phytopathology 85:1436-1440.
15. Hadar, Y., G. E. Harman, and A. G. Taylor. 1984. Evaluation of Trichoderma koningii and T. harzianum from New York soils for biological control of seed rot caused by Pythium spp. Phytopathology 74:106-110.
16. Harman, G. E., I. Chet, and R. Baker. 1981. Factors affecting Trichoderma hamatum applied to seeds as a biocontrol agent. Phytopathology 71:569-572.
17. Hubbard, J. P., G. E. Harman, and Y. Hadar. 1983. Effect of soilborne Pseudomonas spp. on the biological control agent, Trichoderma hamatum, on pea seeds. Phytopathology 73:655-659.
18. Knudsen, G. R., and L. Bin. 1990. Effects of temperature, soil moisture, and wheat bran on growth of Trichoderma harzianum from alginate pellets. Phytopathology 80:724-727.
19. Knudsen, G. R., D. J. Eschen, L. M. Dandurand, and L. Bin. 1991. Potential for biocontrol of Sclerotinia sclerotiorum through colonization of sclerotia by Trichoderma harzianum. Plant Dis. 75:466-470.
20. Kuter, G. A., E. B. Nelson, H. A. J. Hoitink, and L. V. Madden. 1983. Fungal population in container media amended with composted hardwood bark suppressive and conductive to Rhizoctonia damping-off. Phytopathology 73: 1450-1456.
21. Lo, C.-T., E. B. Nelson, C. K. Hayes, and G. E. Harman. 1998. Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology 88: 129-136.
22. Migheli, Q., A. Herrera-Estrella, M. Avataneo, and M. L. Gullino. 1994. Fate of transformed Trichoderma harzianum in the phylloplane of tomato plants. Mol. Ecol. 3:153-159.
23. Papavizas, G. C. 1985. Trichoderma and Gliocladium: biology, ecology, and potential for biocontrol. Annu. Rev. Phytopathol. 23:23-54.
24. Papavizas, G. C., J. A. Lewis, and T. H. Abd-El Moity. 1982. Evaluation of new biotypes of Trichoderma harzianum for tolerance to benomyl and enhanced biocontrol capabilities. Phytopathology 72:126-132.
25. Papavizas, G. C., and R. D. Lumsden. 1982. Improved medium for isolation of Trichoderma spp. from soil. Plant Dis. 66:1019-1020.
26. Penttilä, M., H. Nevalainen, M. Rättö, E. Salminen, and J. Knowles. 1987. A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61:155-164.
27. Punt, P. J., R. P. Oliver, M. A. Dingemense, P. H. Pouwels, and C. A. M. J. J. van den Hondel. 1987. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-124.
28. Raeder, U., and P. Broda. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1:17-20.
29. Roberts, I. N., R. P. Oliver, P. J. Punt, and C. A. M. J. J. van den Hondel. 1989. Expression of Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Curr. Genet. 15:177-180.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sheen, J., S. Hwang, Y. Niwa, H. Kobayaski, and D. W. Galbrailh. 1995 Green-fluorescent protein as a new vital marker in plant cells. Plant J. 8:777-784.
32. Shi, Z., D. Christian, and H. Leung. 1995. Enhanced transformation in Magnaporthe grisea by restriction enzyme mediated integration of plasmid DNA. Phytopathology 85:329-333.
33. Sivan, A., T. E. Stasz, M. Hemmat, C. K. Hayes, and G. E. Harman. 1992. Transformation of Trichoderma spp. with plasmids conferring hygromycin B resistance. Mycologia 84:687-694.
34. Spellig, T., A. Bottin, and R. Kahmann. 1996. Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Mol. Gen. Genet. 252:503-509.
35. Stasz, T. E., G. E. Harman, and N. F. Weeden. 1988. Protoplast preparation and fusion in two biocontrol stains of Trichoderma harzianum. Mycologia 80: 141-150.
36. Thrane, C., M. Lübeck, H. Green, Y. Degefu, S. Allerup, U. Thrane, and D. F. Jensen. 1995. A tool for monitoring Trichoderma harzianum. I. Transformation with the GUS gene by protoplast technology. Phytopathology 85:1428-1435.
37. Vanden Wymelenberg, A. J., D. Cullen, R. N. Spear, B. Schoenike, and J. H. Andrews. 1997. Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces. BioTechniques 23:686-690.