Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy

European Biophysics Journal

Volumn 31, Issue 3, 2002, Pages 179-184

  Jermutus, Lutz ^a,c,d,e   Kolly, Reto ^a,c,d,e   Földes Papp, Zeno ^b,c,d,e   Hanes, Jozef ^a,c,d,e   Rigler, Rudolf ^b,c,d,e   Plückthun, Andreas ^a,c,d,e  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b KAROLINSKA INSTITUTE   (Sweden)

^c MEDIMMUNE LTD   (United Kingdom)

^d UNIVERSITY OF GRAZ   (Austria)

^e Evotec Technologies GmbH   (Germany)

Author keywords

Fluorescence correlation spectroscopy; Ribosome display; scFv; Single molecule analysis; Single chain antibody fragment

Indexed keywords

ANTIGEN; PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; FLUORESCENCE; IN VITRO STUDY; LIGAND BINDING; MATHEMATICAL ANALYSIS; MOLECULE; NONHUMAN; PROTEIN PURIFICATION; RIBOSOME; SPECTROSCOPY; STOP CODON;

EID: 0036619434     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-001-0204-0     Document Type: Article

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