Reverse genetics of essential genes in tissue-culture cells: 'Dead cells talking'

Trends in Cell Biology

Volumn 12, Issue 6, 2002, Pages 281-287

  Hudson, Damien F ^a   Morrison, Ciaran ^a   Ruchaud, Sandrine ^a   Earnshaw, William C ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DOUBLE STRANDED RNA; GENE PRODUCT; MUTANT PROTEIN; PROTEIN;

B LYMPHOCYTE; CAENORHABDITIS ELEGANS; CELL LINE; CHICKEN; CYTOLOGY; DROSOPHILA; EMBRYO CELL; ESSENTIAL GENE; EXPERIMENTAL DESIGN; FIBROBLAST; GENE DISRUPTION; GENE MUTATION; GENE SEQUENCE; GENE SILENCING; GENE TARGETING; GENETIC CODE; GENOMICS; HUMAN; KNOCKOUT GENE; MAMMAL CELL; METAZOON; MOUSE MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; REVIEW; SOMATIC CELL; STEM CELL; TISSUE CULTURE CELL; VERTEBRATE;

ANIMALS; B-LYMPHOCYTES; GENE DELETION; GENE SILENCING; GENE TARGETING; GENES, ESSENTIAL; GENES, LETHAL; HUMANS; MUTAGENESIS, SITE-DIRECTED; RNA, SMALL INTERFERING; RNA, UNTRANSLATED; STEM CELLS;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; GALLUS GALLUS; MAMMALIA; METAZOA; VERTEBRATA;

EID: 0036607092     PISSN: 09628924     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0962-8924(02)02281-X     Document Type: Review

References (40)

1. Controlling your losses: Conditional gene silencing in mammals
2. Antisense oligonucleotides: Promise and reality
3. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
4. Construction by gene targeting in human cells of a 'conditional' CDC2 mutant that rereplicates its DNA
5. 14-3-3Sigma is required to prevent mitotic catastrophe after DNA damage
6. Cyclophilin A regulates HIV-1 infectivity, as demonstrated by gene targeting in human T cells
7. CIP1/WAF1 gene in normal diploid human fibroblasts
8. Analysis of biological selections for high-efficiency gene targeting
9. Gene targeting in human cells without isogenic DNA
10. Increased ratio of targeted to random integration after transfection of chicken B cell lines
11. Homologous DNA recombination in vertebrate cells
12. Requirement of the activation-induced deaminase (AID) gene for immunoglobulin
13. Genetic analysis of homologous DNA recombination in vertebrate somatic cells
14. CAD/DFF40 nuclease is dispensable for high molecular weight DNA cleavage and stage I chromatin condensation in apoptosis
15. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
16. Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability
17. Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death
18. Efficient conditional mutation of the vertebrate CENP-C gene
19. Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells
20. Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells
21. Recycling selectable markers in mouse embryonic stem cells
22. The length of homology required for gene targeting in embryonic stem cells
23. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus
24. Dicistronic targeting constructs: Reporters and modifiers of mammalian gene expression
25. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs
26. Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination and predisposition to cancer
27. Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes
28. Conditional gene targeting
29. Engineering mouse chromosomes with Cre-loxP: Range, efficiency, and somatic applications
30. Steroid-binding domains for regulating the functions of heterologous proteins in cis
31. Heat-inducible degron: A method for constructing temperature-sensitive mutants
32. Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins
33. RNA-triggered gene silencing
34. RNA interference - 2001
35. A short primer on RNAi: RNA-directed RNA polymerase acts as a key catalyst
36. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
37. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
38. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
39. Identification of essential genes in cultured mammalian cells using small interfering RNAs
40. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells