A distinct bipartite motif is required for the localization of inhibitory κB-like (IκBL) protein to nuclear speckles

Biochemical Journal

Volumn 361, Issue 3, 2002, Pages 489-496

  Semple, Jennifer I ^a   Brown, Stephanie E ^a   Sanderson, Christopher M ^a   Campbell, R Duncan ^a  

^a MRC UK HGMP Resource Centre   (United Kingdom)

Author keywords

Ankyrin; I B; MHC; Nuclear localization signal; Splicing

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; CELLS; MUTAGENESIS;

NUCLEAR SPECKLE;

PROTEINS;

ANKYRIN; I KAPPA B; LEUCINE ZIPPER PROTEIN; METHIONINE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS; CONTROLLED STUDY; HUMAN; HUMAN CELL; MAJOR HISTOCOMPATIBILITY COMPLEX; MAMMAL CELL; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN MOTIF;

ALTERNATIVE SPLICING; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ANKYRINS; BLOTTING, WESTERN; CELL LINE; CELL NUCLEUS; COS CELLS; EXONS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; I-KAPPA B PROTEINS; LEUCINE ZIPPERS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NUCLEAR LOCALIZATION SIGNALS; PROTEIN STRUCTURE, TERTIARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA SPLICING; TRANSFECTION; TUMOR CELLS, CULTURED;

MAMMALIA;

EID: 0036471943     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3610489     Document Type: Article

References (41)

1. Characterization of a novel gene in the human major histocompatibility complex that encodes a potential new member of the IκB family of proteins
2. A second susceptibility gene for developing rheumatoid arthritis in the human MHC is localized within a 70-kb interval telomeric of the TNF genes in the HLA class III region
3. Susceptibility to severe ulcerative colitis is associated with polymorphism in the central MHC gene IKBL
4. IκB proteins: Structure, function and regulation
5. Common structural constituents confer IκB activity to NF-κB p105 and IκB/MAD-3
6. Phosphorylation of IκBα in the C-terminal Pest domain by casein kinase II affects intrinsic protein stability
7. Structure of an IκBα/NF-κB complex
8. Nuclear uptake control of NF-κB by MAD-3, an IκB protein present in the nucleus
9. Rapid proteolysis of IκB-α is necessary for activation of transcription factor NF-κB
10. Mutual regulation of the transcriptional activator NF-κB and its inhibitor, IκB-α
11. Nuclear localization of IκBα promotes active transport of NF-κB from the nucleus to the cytoplasm
12. Nuclear localization of IκBα is mediated by the second ankyrin repeat: The IκBα ankyrin repeats define a novel class of cis-acting nuclear import sequences
13. Identification of a sialidase encoded in the human major histocompatibility complex
14. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
16. Preparation of nuclei from HeLa cell suspension cultures by osmotic swelling
17. The G11 gene located in the major histocompatibility complex encodes a novel nuclear serine/threonine protein kinase
18. Characterization of a human lysophosphatidic acid acyltransferase that is encoded by a gene located in the class III region of the human major histocompatibility complex
19. Nuclear eukaryotic initiation factor 4E (eIF4E) colocalizes with splicing factors in speckles
20. A catalogue of splice junction sequences
21. Initiation of translation in prokaryotes and eukaryotes
22. Interpreting cDNA sequences: Some insights from studies on translation
23. Nuclear organization and gene expression
24. Purification and biochemical characterization of interchromatin granule clusters
25. Finding nuclear localization signals
26. C/EBP proteins contain nuclear localization signals imbedded in their basic regions
27. An RNA recognition motif (RRM) is required for the IQDocalization of PTB-associated splicing factor (PSF) to subnuclear speckles
28. Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity
29. An amino acid sequence motif sufficient for subnuclear localization of an arginine/serine-rich splicing factor
30. The Sm core domain mediates targeting of U1 snRNP to subnuclear compartments involved in transcription and splicing
31. Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1
32. Identification of a sequence element directing a protein to nuclear speckles
33. Nuclear pre-mRNA compartmentalization: Trafficking of released transcripts to splicing factor reservoirs
34. The dynamics of a pre-mRNA splicing factor in living cells
35. The ankyrin repeat: A diversity of interactions on a common structural framework
36. Nuclear import of IκBα is accomplished by a ran-independent transport pathway
37. Characterization of IκBα nuclear import pathway
38. Inducible nuclear expression of newly synthesized IκBα negatively regulates DNA-binding and transcriptional activities of NF-κB
39. IκBα can localize in the nucleus but shows no direct transactivation potential
40. Protein-tyrosine phosphatase inhibitors block tumor necrosis factor-dependent activation of the nuclear transcription factor NF-κB