Nuclear import of IκBα is accomplished by a Ran-independent transport pathway

Molecular and Cellular Biology

Volumn 20, Issue 5, 2000, Pages 1571-1582

  Sachdev, Shrikesh ^a,b   Bagchi, Sriparna ^a   Zhang, Donna D ^a   Mings, Angela C ^a   Hannink, Mark ^a  

^a University of Missouri   (United States)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ANKYRIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; KARYOPHERIN; PROTEIN INHIBITOR; I KAPPA B; RAN PROTEIN;

ARTICLE; CELL TRANSPORT; GENE EXPRESSION; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN TRANSPORT; TEMPERATURE DEPENDENCE; CELL NUCLEUS; METABOLISM; SIGNAL TRANSDUCTION; TRANSPORT AT THE CELLULAR LEVEL;

BIOLOGICAL TRANSPORT; CELL NUCLEUS; HELA CELLS; HUMANS; I-KAPPA B PROTEINS; RAN GTP-BINDING PROTEIN; SIGNAL TRANSDUCTION;

EID: 0033622134     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.5.1571-1582.2000     Document Type: Article

References (48)

1. Adam, S. A., R. S. Marr, and L. Gerace. 1990. Nuclear protein import in permeubilized cells requires soluble cytoplasmic factors. J. Cell Biol. 111: 807-816.
2. Arenzana-Seisdedos, F., J. Thompson, M. S. Rodriguez, F. Bachelerie, D. Thomas, and R. T. Hay. 1995. Inducible nuclear expression of newly synthesized IκBu negatively regulates DNA-binding and transcriptional activities of NF-κB. Mol. Cell. Biol. 15:2689-2696.
3. Arenzana-Seisdedos, F., P. Turpin, M. Rodriguez, D. Thomas, R. T. Hay, J.-L. Virelizier, and C. Dargemont. 1997. Nuclear localization of IκBα promotes active transport of NF-κB from the nucleus to the cytoplasm. J. Cell Sci. 110:369-378.
4. Askjaer, P., T. H. Jensen, J. Nilsson, L. Englmeier, J. Kjems. 1998. The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP. J. Biol. Chem. 273:33414-33422.
5. Baldwin, A. S., Jr. 1996. The NF-κB and IκB proteins: new discoveries and insights. Annu. Rev. Immunol. 14:649-681.
6. Bischoff, F. R., and D. Gorlich. 1997. RanBP1 is crucial for the release of RanGTP from importin-β-related nuclear transport factors. FEBS Lett. 419: 249-254.
7. Chi, N., E. Adam, and S. Adam. 1995. Sequence and characterization of cytoplasmic nuclear import factor p97. J. Cell Biol. 130:265-274.
8. Davis, L. I. 1995. The nuclear pore complex. Annu. Rev. Biochem. 64:865-896.
9. Davis, N., S. Ghosh, D. L. Simmons, P. Tempst, H. C. Liou, D. Baltimore, and H. R. Bose, Jr. 1991. Rel-associated pp40: an inhibitor of the Rel family of transcription factors. Science 253:1268-1271.
10. Englmeier, L., J.-C. Olivo, and I. W. Mattaj. 1999. Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis. Curr. Biol. 9:30-41.
11. Fagotto, F., U. Gluck, and B. M. Gumbiner. 1998. Nuclear localization signal-independent and importin/karyopherin-independent nuclear import of β-catenin. Curr. Biol. 8:181-190.
12. Fischer, U., J. Huber, W. C. Bodens, I. W. Mattaj, and R. Luhrmann. 1995. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82:475-484.
13. Fornerod, M., M. Ohno, M. Yoshida, and I. W. Mattaj. 1997. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90:1051-1060.
14. Fukuda, M., S. Asano, T. Nakamura, M. Adachi, M. Yoshida, M. Yanagida, and E. Nishida. 1997. CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature (London) 390:308-311.
15. Goldfarb, D. S., J. Gariepy, G. Schoolnik, and R. D. Kornberg. 1986. Synthetic peptides as nuclear localization signals. Nature (London) 322:641-644.
16. Gorlich, D., S. Prehn, R. A. Laskey, and E. Hartmann. 1994. Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79:767-778.
17. Gorlich, U., S. Kostka, R. Kraft, C. Dingwall, R. Laskey, E. Hartmann, and S. Prehn. 1995. Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr. Biol. 5:383-392.
18. Gorlich, D., G. Vogel, A. Mills, E. Hartmann, and R. Laskey. 1995. Distinct functions for the two importin subunits in nuclear protein import. Nature (London) 377:246-248.
19. Hopper, A. K., H. M. Traglia, and R. W. Dunst. 1990. The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and is apparently excluded from the nucleus. J. Cell Biol. 111:309-321.
20. Huxford, T., D.-B. Huang, S. Malek, and G. Ghosh. 1998. The crystal structure of the IκBα/NF-κB complex reveals mechanisms of NF-κB inactivation. Cell 95:759-770.
21. Izaurralde, E., U. Kutay, C. von Kobbe, I. W. Mattaj, and D. Gorlich. 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16:6535-6547.
22. Jacobs, M. D. and S. C. Harrison. 1998. Structure of an IκBα/NF-κB complex. Cell 95:749-758.
23. Jenkins, Y., M. McEntee, K. Weis, and W. C. Greene. 1998. Characterization of HIV-1 Vpr nuclear import: analysis of signals and pathways. J. Cell Biol. 143:875-885.
24. Johnson, C., D. Van Antwerp, and T. J. Hope. 1999. An N-terminal nuclear export signal is required for the nucleocytoplasmic shuttling of IκBα. EMBO J. 18:6682-6693.
25. Kalderon, D., B. L. Roberts, W. D. Richardson, and A. E. Smith. 1984. A short amino acid sequence able to specify nuclear location. Cell 39:499-509.
26. Kehlenbach, R. H., A. Dickmanns, and L. Gerace. 1998. Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT in vitro. J. Cell Biol. 141:863-874.
27. Kose, S., N. Imamoto, T. Tachibana, T. Shimamoto, and Y. Yoneda. 1997. Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex. J. Cell Biol. 139:841-849.
28. Kutay, U., E. Izaurralde, F. R. Bischoff, I. W. Mattaj, and D. Gorlich. 1997. Dominant-negative mutants of importin-β block multiple pathways of import and export through the nuclear pore complex. EMBO J. 16:1153-1163.
29. Kutay, U., F. Bischoff, S. Kostka, R. Kraft, and D. Gorlich. 1997. Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 90:1061-1071.
30. Mattaj, I. W., and L. Englmeier. 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67:265-306.
31. 2 bind nuclear localization signal and β interacts with peptide repeat containing nucleoporins. Proc. Natl. Acad. Sci. USA 92:6532-6536.
32. Nakielny, S., and G. Dreyfuss. 1997. Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis. Curr. Biol. 8:89-95.
33. Nishi, K., M. Yoshida, D. Fujiwara, M. Nishikawa, S. Horinouchi, and T. Beppu. 1994. Leptomycin B targets a regulatory cascade of crml, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression. J. Biol. Chem. 269:6320-6324.
34. Ohtsubo, M., H. Okazaki, and T. Nishimoto. 1989. The RCC1 protein, a regulator for the onset of chromosome condensation, locates in the nucleus and hinds DNA. J. Cell Biol. 109:1389-1397.
35. Pollard, V. W., W. M. Michael, S. Nakielny, M. C. Siomi, F. Wang, and G. Dreyfuss. 1996. A novel receptor-mediated nuclear protein import pathway. Cell 86:985-994.
36. Radu, A., G. Blobel, and M. S. Moore. 1995. Identification of a protein complex that is required for nuclear-protein import and mediates docking of import substrate to distinct nucleoporins. Proc. Natl. Acad. Sci. USA 92: 1769-1773.
37. Radu, A., M. S. Moore, and G. Blobel. 1995. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81:215-222.
38. Rexach, M., and G. Blobel. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors and nucleoporins. Cell 83:683-692.
39. Richards, S. A., K. L. Carey, and I. G. Macara. 1997. Requirement for guanosine triphosphate-bound Ran for signal-mediated nuclear protein export. Science 276:1842-1844.
40. Rodriguez, M. S., J. Thompson, R. T. Hay, and C. Dargemont. 1999. Nuclear retention of IκBα protects it from signal-induced degradation and inhibits nuclear factor κB transcriptional activation. J. Biol. Chem. 274:9108-9115.
41. Sachdev, S., E. M. Rottjakob, J. A. Diehl, and M. Hannink. 1995. IκBα mediated inhibition of nuclear transport and DNA-binding by Rel proteins are separable functions: phosphorylation of C-terminal serine residues of IκBα is specifically required for inhibition of DNA-binding. Oncogene 11: 811-823.
42. Sachdev, S., A. Hoffman, and M. Hannink. 1998. Nuclear localization of IκBα is mediated by the second ankyrin repeat: the IκBα ankyrin repeats define a novel class of cis-acting nuclear import sequences. Mol. Cell. Biol. 18:2524-2534.
43. Sachdev, S., and M. Hannink. 1998. Loss of IκBα-mediated control over nuclear import and DNA binding enables oncogenic activation of c-Rel. Mol. Cell. Biol. 18:5445-5456.
44. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
45. Stade, K., C. S. Ford, C. Guthrie, and K. Weis. 1997. Exportin 1 (Crm1p) is an essential nuclear export factor. Cell 90:1041-1050.
46. Turpin, P., R. T. Hay, and C. Dargemont. 1999. Characterization of IκBα nuclear import pathway. J. Biol. Chem. 274:6804-6812.
47. Wen, W., J. L. Meinkoth, R. Y. Tsien, and S. S. Taylor. 1995. Identification of a signal for rapid export of proteins from the nucleus. Cell 82:463-474.
48. Yokoja, F., N. Imammoto, T. Tachibana, and Y. Yoneda. 1999. β-Catenin can be transported into the nucleus in a Ran-unassisted manner. Mol. Biol. Cell 10:1119-1131.