Characterization of monomeric and dimeric forms of recombinant Sml1p-histag protein by electrospray mass spectrometry

Analytical Biochemistry

Volumn 301, Issue 1, 2002, Pages 35-48

  Uchiki, Tomoaki ^a   Hettich, Robert ^a   Gupta, Vibha ^b   Dealwis, Chris ^b  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Collisional dissociation; Disulfide bond determination; Electrospray; Enzyme inhibition; Mass spectrometry; Proteolytic digests; Recombinant protein

Indexed keywords

BIOSYNTHESIS; CHEMICAL MODIFICATION; COVALENT BONDS; ENZYME INHIBITION; MASS SPECTROMETRY; METAL IONS; METALS; SULFUR COMPOUNDS;

COLLISIONAL DISSOCIATION; DISULFIDE BONDS; ELECTROSPRAY MASS SPECTROMETRY; ELECTROSPRAYS; ENZYME COMPLEXES; METAL ION BINDING; PROTEOLYTIC DIGESTS; RIBONUCLEOTIDE REDUCTASE;

RECOMBINANT PROTEINS;

CHECKPOINT KINASE 2; DEOXYRIBONUCLEOTIDE; DIMER; DNA; DODECYL SULFATE SODIUM; GENE PRODUCT; MEC1 PROTEIN; METAL ION; MONOMER; POLYACRYLAMIDE GEL; RECOMBINANT PROTEIN; RIBONUCLEOTIDE REDUCTASE; SML1P HISTAG PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; ANALYTIC METHOD; ARTICLE; CELL CYCLE; CONTROLLED STUDY; DISULFIDE BOND; DNA REPAIR; DNA SYNTHESIS; ENZYME INHIBITION; INTERMETHOD COMPARISON; MASS SPECTROMETRY; METAL BINDING; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

STAPHYLOCOCCUS PHAGE 3A;

EID: 0036467707     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5492     Document Type: Article

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