Attomole protein characterization by capillary electrophoresis-mass spectrometry

Science

Volumn 273, Issue 5279, 1996, Pages 1199-1202

  Valaskovic, Gary A ^a   Kelleher, Neil L ^a   McLafferty, Fred W ^a  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBONATE DEHYDRATASE; PLASMA PROTEIN;

ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DISSOCIATION; ERYTHROCYTE; FOURIER TRANSFORMATION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION;

EID: 0029841145     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5279.1199     Document Type: Article

References (38)

1. K. Sauer, Methods Enzymol. 246, 1 (1995); B. Herman and K. Jacobsen, Eds., Optical Microscopy for Biology (Wiley, New York, 1990); J. K. Foskett and S. Grinstein, Eds., Noninvasive Techniques in Cell Biology (Wiley-Liss, New York, 1990), vol. 9.
2. K. Sauer, Methods Enzymol. 246, 1 (1995); B. Herman and K. Jacobsen, Eds., Optical Microscopy for Biology (Wiley, New York, 1990); J. K. Foskett and S. Grinstein, Eds., Noninvasive Techniques in Cell Biology (Wiley-Liss, New York, 1990), vol. 9.
3. K. Sauer, Methods Enzymol. 246, 1 (1995); B. Herman and K. Jacobsen, Eds., Optical Microscopy for Biology (Wiley, New York, 1990); J. K. Foskett and S. Grinstein, Eds., Noninvasive Techniques in Cell Biology (Wiley-Liss, New York, 1990), vol. 9.
4. J. W. Jorgenson and K. D. Lukacs, Science 222, 266 (1983).
5. R. T. Kennedy, M. D. Oates, B. R. Cooper, B. Nickerson, J. W. Jorgenson, ibid. 246, 57 (1989).
6. A. G. Ewing, T. G. Strein, Y. Yi-Lau, Acc. Chem. Res. 25, 440 (1992).
7. E. S. Yeung, ibid. 27, 409 (1994).
8. B. L. Karger, Y.-H. Chu, F. Foret, Annu. Rev. Biophys. Biomol. Struct. 24, 579 (1995).
9. H. A. Fishman, O. Owar, R. H. Schaller, R. N. Zare, Proc. Natl. Acad. Sci. U.S.A. 92, 7877 (1995).
10. J. B. Fenn, M. Mann, C. K. Meng, S. F. Wong, C. M. Whitehouse, Science 246, 64 (1989).
11. R. D. Smith, J. H. Wahl, D. R. Goodlett, S. A. Hofstadler, Anal. Chem. 65, 574A (1993).
12. S. A. Hofstadler et al., Rapid Commun. Mass Spectrom. 10, 919 (1996).
13. M. S. Wilm and M. Mann, Int. J. Mass Spectrom. Ion Processes 136, 167 (1994).
14. G. A. Valaskovic, N. L. Kelleher, D. P. Little, D. J. Aaserud, F. W. McLafferty, Anal. Chem. 67, 3802 (1995).
15. J. H. Wahl, D. R. Goodlett, H. R. Udseth, R. D. Smith, Electrophoresis 14, 448 (1993).
16. G. A. Valaskovic and F. W. McLafferty, Rapid Commun. Mass Spectrom. 10, 825 (1996).
17. S. C. Beu, M. W. Senko, J. P. Quinn, F. M. Wampler, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 4, 557 (1993); F. W. McLafferty, Acc. Chem. Res. 27 379 (1994).
18. S. C. Beu, M. W. Senko, J. P. Quinn, F. M. Wampler, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 4, 557 (1993); F. W. McLafferty, Acc. Chem. Res. 27 379 (1994).
19. M. W. Senko, S. C. Beu, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 6, 52 (1995); ibid., p. 229.
20. M. W. Senko, S. C. Beu, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 6, 52 (1995); ibid., p. 229.
21. M. Mann, C. K. Meng, J. B. Fenn, Anal. Chem. 61, 1702 (1989).
22. J. A. Loo and R. Pesch, ibid. 66, 3659 (1994); M. R. Emmett and R. M. Caprioli, J. Am. Soc. Mass Spectrom. 5, 605 (1994).
23. J. A. Loo and R. Pesch, ibid. 66, 3659 (1994); M. R. Emmett and R. M. Caprioli, J. Am. Soc. Mass Spectrom. 5, 605 (1994).
24. Fresh human blood (5 μl) that should contain ≈200 pmol of CA [R. B. Pennell, in The Red Blood Cell, D. N. Surgenor, Ed. (Academic Press, New York, 1974), vol. 1, pp. 93-105] was extracted with methanol-water-carbon tetrachloride [S. Lindskog. Biochim. Biophys. Acta 39, 218 (1960); ≈ 80% efficiency] without electrophoretic purification; the aqueous layer was desalted twice (≈95% efficiency each) by ultrafiltration (10 KD), the retentate was diluted (10 mM acetic acid) to 400 μl. and 18 pl was injected.
25. Fresh human blood (5 μl) that should contain ≈200 pmol of CA [R. B. Pennell, in The Red Blood Cell, D. N. Surgenor, Ed. (Academic Press, New York, 1974), vol. 1, pp. 93-105] was extracted with methanol-water-carbon tetrachloride [S. Lindskog. Biochim. Biophys. Acta 39, 218 (1960); ≈ 80% efficiency] without electrophoretic purification; the aqueous layer was desalted twice (≈95% efficiency each) by ultrafiltration (10 KD), the retentate was diluted (10 mM acetic acid) to 400 μl. and 18 pl was injected.
26. K. T. Flaming and D. G. Brown, Advanced Micropipette Techniques for Cell Physiology (Wiley-Interscience, Chichester, 1986); B. Sakmann and E. Neher, Eds., Single-Channel Recording (Plenum, New York, 1983).
27. K. T. Flaming and D. G. Brown, Advanced Micropipette Techniques for Cell Physiology (Wiley-Interscience, Chichester, 1986); B. Sakmann and E. Neher, Eds., Single-Channel Recording (Plenum, New York, 1983).
28. A nonredundant database is maintained by C. Sańders at EMBL and can be downloaded by FTP from ebi.ac.uk/pub/databases/Peptidesearch.
29. E. Mørtz et al., Proc. Natl. Acad. Sci. U.S.A. 93, 8264 (1996).
30. N. L. Kelleher, C. A. Costello, T. P. Begley, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 6, 981 (1995); J. P. Speir, M. W. Senko, D. P. Little, J. A. Loo, F. W. McLafferty, J. Mass Spectrom. 30, 39 (1995).
31. N. L. Kelleher, C. A. Costello, T. P. Begley, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 6, 981 (1995); J. P. Speir, M. W. Senko, D. P. Little, J. A. Loo, F. W. McLafferty, J. Mass Spectrom. 30, 39 (1995).
32. J. A. Loo, C. G. Edmonds, R. D. Smith, Anal. Chem. 63, 2488 (1991).
33. P. Roepstorff and J. Fohlman, Biomed. Mass Spectrom. 11, 601 (1984).
34. M. Mann and M. Wilm, Anal. Chem. 66, 4390 (1994).
35. S. A. Hofstadler, J. H. Wahl, J. E. Bruce, R. D. Smith, J. Am. Chem. Soc. 115, 6983 (1993).
36. R. F. Curl and R. E. Smalley, Science 242, 1017 (1988).
37. D. P. Little, D. J. Aaserud, G. A. Valaskovic, F. W. McLafferty, J. Am. Chem. Soc., in press.
38. We thank D. J. Aaserud, Z. Guan, E. K. Fridriksson, H. Lin, T. D. Wood, and M. W. Senko for advice and experimental assistance and G. H. Morrison and M. Holton for tip fabrication equipment. This work was supported under NIH grant GM16609 (to F.W.M.) and cell and molecular biology training grant 08-T2GM07273 (to N.L.K.).