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Volumn 273, Issue 5279, 1996, Pages 1199-1202

Attomole protein characterization by capillary electrophoresis-mass spectrometry

Author keywords

[No Author keywords available]

Indexed keywords

CARBONATE DEHYDRATASE; PLASMA PROTEIN;

EID: 0029841145     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5279.1199     Document Type: Article
Times cited : (352)

References (38)
  • 1
    • 0028941210 scopus 로고
    • K. Sauer, Methods Enzymol. 246, 1 (1995); B. Herman and K. Jacobsen, Eds., Optical Microscopy for Biology (Wiley, New York, 1990); J. K. Foskett and S. Grinstein, Eds., Noninvasive Techniques in Cell Biology (Wiley-Liss, New York, 1990), vol. 9.
    • (1995) Methods Enzymol. , vol.246 , pp. 1
    • Sauer, K.1
  • 2
    • 0028941210 scopus 로고
    • Wiley, New York
    • K. Sauer, Methods Enzymol. 246, 1 (1995); B. Herman and K. Jacobsen, Eds., Optical Microscopy for Biology (Wiley, New York, 1990); J. K. Foskett and S. Grinstein, Eds., Noninvasive Techniques in Cell Biology (Wiley-Liss, New York, 1990), vol. 9.
    • (1990) Optical Microscopy for Biology
    • Herman, B.1    Jacobsen, K.2
  • 3
    • 0028941210 scopus 로고
    • Wiley-Liss, New York
    • K. Sauer, Methods Enzymol. 246, 1 (1995); B. Herman and K. Jacobsen, Eds., Optical Microscopy for Biology (Wiley, New York, 1990); J. K. Foskett and S. Grinstein, Eds., Noninvasive Techniques in Cell Biology (Wiley-Liss, New York, 1990), vol. 9.
    • (1990) Noninvasive Techniques in Cell Biology , vol.9
    • Foskett, J.K.1    Grinstein, S.2
  • 18
    • 0000323481 scopus 로고
    • S. C. Beu, M. W. Senko, J. P. Quinn, F. M. Wampler, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 4, 557 (1993); F. W. McLafferty, Acc. Chem. Res. 27 379 (1994).
    • (1994) Acc. Chem. Res. , vol.27 , pp. 379
    • McLafferty, F.W.1
  • 20
    • 58149208985 scopus 로고    scopus 로고
    • M. W. Senko, S. C. Beu, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 6, 52 (1995); ibid., p. 229.
    • J. Am. Soc. Mass Spectrom. , pp. 229
  • 22
    • 0028149331 scopus 로고
    • J. A. Loo and R. Pesch, ibid. 66, 3659 (1994); M. R. Emmett and R. M. Caprioli, J. Am. Soc. Mass Spectrom. 5, 605 (1994).
    • (1994) Anal. Chem. , vol.66 , pp. 3659
    • Loo, J.A.1    Pesch, R.2
  • 24
    • 0002442393 scopus 로고
    • D. N. Surgenor, Ed. Academic Press, New York
    • Fresh human blood (5 μl) that should contain ≈200 pmol of CA [R. B. Pennell, in The Red Blood Cell, D. N. Surgenor, Ed. (Academic Press, New York, 1974), vol. 1, pp. 93-105] was extracted with methanol-water-carbon tetrachloride [S. Lindskog. Biochim. Biophys. Acta 39, 218 (1960); ≈ 80% efficiency] without electrophoretic purification; the aqueous layer was desalted twice (≈95% efficiency each) by ultrafiltration (10 KD), the retentate was diluted (10 mM acetic acid) to 400 μl. and 18 pl was injected.
    • (1974) The Red Blood Cell , vol.1 , pp. 93-105
    • Pennell, R.B.1
  • 25
    • 0001293394 scopus 로고
    • Fresh human blood (5 μl) that should contain ≈200 pmol of CA [R. B. Pennell, in The Red Blood Cell, D. N. Surgenor, Ed. (Academic Press, New York, 1974), vol. 1, pp. 93-105] was extracted with methanol-water-carbon tetrachloride [S. Lindskog. Biochim. Biophys. Acta 39, 218 (1960); ≈ 80% efficiency] without electrophoretic purification; the aqueous layer was desalted twice (≈95% efficiency each) by ultrafiltration (10 KD), the retentate was diluted (10 mM acetic acid) to 400 μl. and 18 pl was injected.
    • (1960) Biochim. Biophys. Acta , vol.39 , pp. 218
    • Lindskog, S.1
  • 27
    • 0003996053 scopus 로고
    • Plenum, New York
    • K. T. Flaming and D. G. Brown, Advanced Micropipette Techniques for Cell Physiology (Wiley-Interscience, Chichester, 1986); B. Sakmann and E. Neher, Eds., Single-Channel Recording (Plenum, New York, 1983).
    • (1983) Single-Channel Recording
    • Sakmann, B.1    Neher, E.2
  • 28
    • 9544223538 scopus 로고    scopus 로고
    • A nonredundant database is maintained by C. Sańders at EMBL and can be downloaded by FTP from ebi.ac.uk/pub/databases/Peptidesearch
    • A nonredundant database is maintained by C. Sańders at EMBL and can be downloaded by FTP from ebi.ac.uk/pub/databases/Peptidesearch.
  • 38
    • 9544251746 scopus 로고    scopus 로고
    • note
    • We thank D. J. Aaserud, Z. Guan, E. K. Fridriksson, H. Lin, T. D. Wood, and M. W. Senko for advice and experimental assistance and G. H. Morrison and M. Holton for tip fabrication equipment. This work was supported under NIH grant GM16609 (to F.W.M.) and cell and molecular biology training grant 08-T2GM07273 (to N.L.K.).


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