Clustered charge-to-alanine mutagenesis of human respiratory syncytial virus L polymerase generates temperature-sensitive viruses

Virology

Volumn 302, Issue 1, 2002, Pages 207-216

  Tang, Roderick S ^a   Nguyen, Nick ^a   Zhou, Helen ^a   Jin, Hong ^a  

^a MEDIMMUNE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; COMPLEMENTARY DNA; LARGE POLYMERASE PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FUNCTION; REPORTER GENE; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY SYSTEM; RNA SYNTHESIS; TEMPERATURE; TEMPERATURE SENSITIVE MUTANT; VIROGENESIS; VIRUS CULTURE; VIRUS MORPHOLOGY; VIRUS MUTATION; VIRUS RECOMBINANT; VIRUS REPLICATION;

GOSSYPIUM HIRSUTUM; HUMAN RESPIRATORY SYNCYTIAL VIRUS; RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS; SIGMODON;

EID: 0036434274     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2002.1596     Document Type: Article

References (46)

1. Belshe, R. B., Mendelman, P. M., Treanor, J., King, J., Gruber, W. C., Piedra, P., Bernstein, D. I., Hayden, F. G., Kotloff, K., Zangwill, K., Iacuzio, D., and Wolff, M. (1998). The efficacy of live attenuated, cold-adapted, trivalent, intranasal influenza virus vaccine in children. N. Engl. J. Med. 338, 1405-1412.
2. Bermingham, A., and Collins, P. L. (1999). The M2-2 protein of human respiratory syncytial virus is a regulatory factor involved in the balance between RNA replication and transcription. Proc. Natl. Acad. Sci. USA 96, 11259-11264.
3. Chandrika, R., Horikami, S. M., Smallwood, S., and Moyer, S. A. (1995). Mutations in conserved domain I of the Sendai virus L polymerase protein uncouple transcription and replication. Virology 213, 352-363.
4. Cheng, X., Zhou, H., Tang, R. S., Munoz, M. G., and Jin, H. (2001). Chimeric subgroup A respiratory syncytial virus with the glycoproteins substituted by those of subgroup B and RSV without the M2-2 gene are attenuated in African green monkeys. Virology 283, 59-68.
5. Collins, P. L., Hill, M. G., Camargo, E., Grosfeld, H., Chanock, R. M., and Murphy, B. R. (1995). Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5′ proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proc. Natl. Acad. Sci. USA 92, 11563-11567.
6. Collins, P. L., Hill, M. G., Cristina, J., and Grosfeld, H. (1996). Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus. Proc. Natl. Acad. Sci. USA 93, 81-85.
7. Collins, P. L., McIntosh, K., and Chanock, R. M. (2001). Respiratory syncytial virus. In "Fields Virology" (B. N. Fields, P. M. Knipe, and P. M. Howley, Eds.), 3rd ed. Lippincott-Raven, Philadelphia.
8. Collins, P. L., Whitehead, S. S., Bukreyev, A., Fearns, R., Teng, M. N., Juhasz, K., Chanock, R. M., and Murphy, B. R. (1999). Rational design of live-attenuated recombinant vaccine virus for human respiratory syncytial virus by reverse genetics. Adv. Virus Res. 54, 423-451.
9. Cortese, C. K., Feller, J. A., and Moyer, S. A. (2000). Mutations in domain V of the Sendai virus L polymerase protein uncouple transcription and replication and differentially affect replication in vitro and in vivo. Virology 277, 387-396.
10. Crowe, J. E., Jr., Bui, P. T., Firestone, C. Y., Connors, M., Elkins, W. R., Chanock, R. M., and Murphy, B. R. (1996). Live subgroup B respiratory syncytial virus vaccines that are attenuated, genetically stable, and immunogenic in rodents and nonhuman primates. J. Infect. Dis. 173, 829-839.
11. Diamond, S. E., and Kirkegaard, K. (1994). Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis. J. Virol. 68, 2233-2243.
12. Falsey, A. R., and Walsh, E. E. (2000). Respiratory syncytial virus infection in adults. Clin. Microbiol. Rev. 13, 371-384.
13. Fearns, R., and Collins, P. L. (1999). Role of the M2-1 transcription antitermination protein of respiratory syncytial virus in sequential transcription. J. Virol. 73, 5852-5864.
14. Feller, J. A., Smallwood, S., Horikami, S. M., and Moyer, S. A. (2000a). Mutations in conserved domains IV and VI of the large (L) subunit of the Sendai virus RNA polymerase give a spectrum of defective RNA synthesis phenotypes. Virology 269, 426-439.
15. Feller, J. A., Smallwood, S., Skiadopoulos, M. H., Murphy, B. R., and Moyer, S. A. (2000b). Comparison of identical temperature-sensitive mutations in the L polymerase proteins of Sendai and parainfluenza 3 viruses. Virology 276, 190-201.
16. Grosfeld, H., Hill, M. G., and Collins, P. L. (1995). RNA replication by respiratory syncytial virus (RSV) is directed by the N, P, and L proteins; transcription also occurs under these conditions but requires RSV superinfection for efficient synthesis of full-length mRNA. J. Virol. 69, 5677-5686.
17. Hall, S. L., Stokes, A., Tierney, E. L., London, W. T., Belshe, R. B., Newman, F. C., and Murphy, B. R. (1992). Cold-passaged human parainfluenza type 3 viruses contain ts and non-ts mutations leading to attenuation in rhesus monkeys. Virus Res. 22, 173-184.
18. Hanley, K. A., Lee, J. J., Blaney, J. E. J., Murphy, B. R., and Whitehead, S. S. (2002). Paired charge-to-alanine mutagenesis of dengue virus type 4 NS5 generates mutants with temperature-sensitive, host range, and mouse attenuation phenotypes. J. Virol. 76, 525-531.
19. Hardy, R. W., and Wertz, G. W. (1998). The product of the respiratory syncytial virus M2 gene ORF1 enhances readthrough of intergenic junctions during viral transcription. J. Virol. 72, 520-526.
20. Hassett, D. E., and Condit, R. C. (1994). Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine. Proc. Natl. Acad. Sci. USA 91, 4554-4558.
21. Jin, H., Cheng, X., Zhou, H., Li, S., and Seddiqui, A. (2000). Respiratory syncytial virus that lacks open reading frame 2 of the M2 gene (M2-2) has altered growth characteristics and is attenuated in rodents. J. Virol. 74, 74-82.
22. Jin, H., Clarke, D., Zhou, H., Coelingh, K., Bryant, M., and Li, S. (1998). Recombinant human respiratory syncytial virus (RSV) from cDNA and construction of subgroup A and B chimeric RSV. Virology 251, 206-214.
23. Juhasz, K., Murphy, B. R., and Collins, P. L. (1999). The major attenuating mutations of the respiratory syncytial virus vaccine candidate cpts530/1009 specify temperature-sensitive defects in transcription and replication and a non-temperature-sensitive alteration in mRNA termination. J. Virol. 73, 5176-5180.
24. Juhasz, K., Whitehead, S. S., Bui, P. T., Biggs, J. M., Boulanger, C. A., Collins, P. L., and Murphy, B. R. (1997). The temperature-sensitive (ts) phenotype of a cold-passaged (cp) live attenuated respiratory syncytial virus vaccine candidate, designated cpts530, results form a single amino acid substitution in the L protein. J. Virol. 71, 5814-5819.
25. Karron, R. A., Wright, P. F., Newman, F. K., Makhene, M., Thompson, J., Samorodin, R., Wilson, M. H., Anderson, E. L., Clements, M. L., Murphy, B. R., et al. (1995). A live human parainfluenza type 3 virus vaccine is attenuated and immunogenic in healthy infants and children. J Infect. Dis. 172, 1445-1450.
26. Lamb, R. A., and Kolakofsky, D. (2001). Paramyxoviridae: The viruses and their replication. In "Fundamental Virology" (B. N. Fields, D. M. Knipe, and P. M. Howley, Eds.). Lippincott-Raven, Philadelphia.
27. Lu, B., Brazas, R., Ma, C. H., Kristoff, T., Cheng, X., and Jin, H. (2002). Identification of temperature-sensitive mutations in the phosphoprotein of respiratory syncytial virus that are likely involved in its interaction with the nucleoprotein. J. Virol. 76, 2871-2871.
28. Marriott, A. C., Wilson, S. D., Randhawa, J. S., and Easton, A. J. (1999). A single amino acid substitution in the phosphoprotein of respiratory syncytial virus confers thermosenstivity in a reconstituted RNA polymerase system. J. Virol. 73, 5162-5165.
29. Parkin, N. T., Chiu, P., and Coelingh, K. (1996). Temperature sensitive mutants of influenza A virus generated by reverse genetics and clustered charged to alanine mutagenesis. Virus Res. 46, 31-44.
30. Poch, O., Blumberg, B. M., Bogueleret, L., and Tordo, N. (1990). Sequence comparison of five polymerase (L proteins) of unsegmented negative-strand RNA viruses: Theoretical assignment of functional domains. J. Gen. Virol. 71, 1153-1162.
31. Poch, O., Sauvaget, I., Delarue, M., and Tordo, N. (1989). Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J. 8, 3867-3874.
32. Prince, G. A., Jenson, A. B., Horswood, R. L., Camargo, E., and Chanock, R. M. (1978). The pathogenesis of respiratory syncytial virus infection in cotton rats. Am. J. Pathol. 93, 771-791.
33. Schnell, M. J., and Conzelmann, K. K. (1995). Polymerase activity of in vitro mutated rabies virus L protein. Virology 214, 522-530.
34. Sidhu, M. S., Menonna, J. P., Cook, S. D., Dowling, P. C., and Udem, S. A. (1993). Canine distemper virus L gene: Sequence and comparison with related viruses. Virology 193, 50-65.
35. Skiadopoulos, M. H., Surman, S. R., St. Claire, M., Elkins, W. R., Collins, P. L., and Murphy, B. R. (1999). Attenutation of the recombinant human parainfluenza virus type 3 cp45 candidate vaccine virus is augmented by importation of the respiratory syncytial virus cpts530 L polymerase mutation. Virology 260, 125-135.
36. Sleat, D. E., and Banerjee, A. K. (1993). Transcriptional activity and mutational analysis of recombinant vesicular stomatitis virus RNA polymerase. J. Virol. 67, 1334-1339.
37. Smallwood, S., Easson, C. D., Feller, J. A., Horikami, S. M., and Moyer, S. A. (1999). Mutations in conserved domain II of the large (L) subunit of the Sendai virus RNA polymerase abolish RNA synthesis. Virology 262, 375-383.
38. Stec, D. S., Hill, M. G., and Collins, P. L. (1991). Sequence analysis of the polymerase L gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. Virology 183, 273-287.
39. Sutter, G., Ohlmann, M., and Erfle, V. (1995). Non-replicating vaccinia vector efficiently expresses bacteriophage T7 RNA polymerase. FEBS Lett. 371, 9-12.
40. Tang, R. S., Nguyen, N., Cheng, X., and Jin, H. (2001). Requirement of cysteines and length of the human respiratory syncytial virus M2-1 protein for protein function and virus viability. J Virol. 75, 11328-11335.
41. Wertman, K. F., Drubin, D. G., and Botstein, D. (1992). Systematic mutational analysis of the yeast ACT1 gene. Genetics 132, 337-350.
42. Whitehead, S. S., Firestone, C., Collins, P. L., and Murphy, B. R. (1998). A single nucleotide substitution in the transcription start signal of the M2 gene of respiratory syncytial virus vaccine candidate cpts248/404 is the major determinant of the temperature-sensitive and attenuation phenotypes. Virology 247, 232-239.
43. Whitehead, S. S., Firestone, C., Karron, R. A., Crowe, J. E. J., Elkins, W. R., Collins, P. L., and Murphy, B. R. (1999). Addition of a missense mutation present in the L gene of respiratory syncytial virus (RSV) cpts530/1030 to RSV vaccine candidate cpts248/404 increases its attenuation and temperature sensitivity. J Virol. 73, 871-877.
44. Wright, P. F., Karron, R. A., Belshe, R. B., Thompson, J., Crowe, J. E., Jr., Boyce, T. G., Halburnt, L. L., Reed, G. W., Whitehead, S. S., Anderson, E. L., Wittek, A. E., Casey, R., Eichelberger, M., Thumar, B., Randolph, V. B., Udem, S. A., Chanock, R. M., and Murphy, B. R. (2000). Evaluation of a live, cold-passaged, temperature-sensitive, respiratory syncytial virus vaccine candidate in infancy. J. Infect. Dis. 182, 1331-1342.
45. Wyatt, L. S., Moss, B., and Rozenblatt, S. (1995). Replication-deficient vaccinia virus encoding bacteriophage T7 RNA polymerase for transient gene expression in mammalian cells. J. Virol. 210, 202-205.
46. Yu, Q., Hardy, R. W., and Wertz, G. W. (1995). Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, L proteins support replication of RS virus genomic RNA analogs and define minimal trans-acting requirements for RNA replication. J Virol. 69, 2412-2419.