Mutations in domain V of the Sendai virus L polymerase protein uncouple transcription and replication and differentially affect replication in vitro and in vivo

Virology

Volumn 277, Issue 2, 2000, Pages 387-396

  Cortese, C K ^a   Feller, J A ^a   Moyer, S A ^a  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; PROTEIN L; RNA DIRECTED RNA POLYMERASE; SPHINGOLIPID ACTIVATOR PROTEIN 2; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CATALYSIS; CONTROLLED STUDY; HUMAN; HUMAN CELL; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RNA SYNTHESIS; SENDAI VIRUS; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; VIRUS NUCLEOCAPSID; VIRUS REPLICATION; VIRUS TRANSCRIPTION;

EID: 0034715824     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2000.0615     Document Type: Article

References (39)

1. Intracellular phosphorylation of the Sendai virus P protein
2. Molecular cloning of natural paramyxovirus copy-back defective interfering RNAs and their expression from DNA
3. The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA
4. In vitro replication of Sendai virus wild-type and defective interfering particle genome RNAs
5. Mutations in conserved domain I of the Sendai virus L polymerase protein uncouple transcription and replication
6. Initiation of vesicular stomatitis virus mutant polR1 transcription internally at the N gene in vitro
7. The Sendai virus P gene expresses both an essential protein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing
8. Paramyxovirus phosphoproteins form homotrimers as determined by an epitope dilution assay, via predicted coiled coils
9. Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis
10. The Sendai virus nucleocapsid exists in at least four different helical states
11. Purification, renaturation, and reconstituted protein kinase activity of the Sendai virus large (L) protein: L protein phosphorylates the NP and P proteins in vitro
12. Mutations in conserved domains IV and VI of the large (L) subunit of the Sendai virus RNA polymerase give a spectrum of defective RNA synthesis phenotypes
13. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
14. Identification and characterization of serine/threonine protein kinase activity intrinsic to the L protein of vesicular stomatitis virus New Jersey
15. Functional analysis of hypomethylation variants of the New Jersey serotype of vesicular stomatitis virus
16. Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine
17. The vesicular stomatitis virus L protein possesses the mRNA methyltransferase activities
18. Complexes of Sendai virus N P-P and P-L proteins are required for defective interfering particle genome replication in vitro
19. The Sendai virus C protein binds the L polymerase protein to inhibit viral RNA synthesis
20. Host range mutants of vesicular stomatitis virus defective in in vitro RNA methylation
21. Alternative amino acids at a single site in the Sendai virus L protein produce multiple defects in RNA synthesis in vitro
22. Amino acid changes in the L polymerase protein of vesicular stomatitis virus which confer aberrant polyadenylation and temperature-sensitive phenotypes
23. Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif
24. Mutagenesis of the L protein encoded by Bunyamwera virus and production of monospecific antibodies
25. Paramyxoviridae: The viruses and their replication
26. Rift Valley fever virus L segment: Correction of the sequence and possible functional role of newly identified regions conserved in the RNA-dependent RNA polymerases
27. An amino-terminal domain of the Sendai virus nucleocapsid protein is required for template function in viral RNA synthesis
28. Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: Theoretical assignment of functional domains
29. The genetics of paramyxoviruses
30. Separate domains of Sendai virus P protein are required for binding to viral nucleocapsids
31. Polymerase activity of in vitro mutated rabies virus L protein
32. Canine distemper virus L gene: Sequence and comparison with related viruses
33. Transcriptional activity and mutational analysis of recombinant vesicular stomatitis virus RNA polymerase
34. Mutations in conserved domain II of the Large (L) subunit of the Sendai virus RNA polymerase abolish RNA synthesis
35. Deletion analysis defines a carboxyl-proximal region of Sendai virus P protein that binds to the polymerase L protein
36. Transcript initiation and 5'-end modifications are separable events during vesicular stomatitis virus transcription
37. On the domain structure, and the polymerization state of the Sendai virus P protein
38. Modified model for the switch from Sendai virus transcription to replication
39. Systematic mutational analysis of the yeast ACT1 gene