Expression of a synthetic E. coli heat-labile enterotoxin B sub-unit (LT-B) in maize

Molecular Breeding

Volumn 10, Issue 4, 2002, Pages 253-265

  Chikwamba, Rachel ^a   McMurray, Jennifer ^a   Shou, Huixia ^a   Frame, Bronwyn ^a   Pegg, Sue Ellen ^a   Scott, Paul ^a   Mason, Hugh ^b   Wang, Kan ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

Author keywords

Functional antigen; Gene expression; LT B; Zea mays

Indexed keywords

ANTIGENS; CELL MEMBRANES; ESCHERICHIA COLI; GRAIN (AGRICULTURAL PRODUCT); IMMUNE SYSTEM; PLANTS (BOTANY);

CAMV 35S PROMOTER; EXTRACTABLES; FUNCTIONAL ANTIGEN; GENES EXPRESSION; HEAT LABILE ENTEROTOXINS; HEAT-LABILE ENTEROTOXIN; IMMUNE RESPONSE; LT-B; MAIZE SEEDS; ZEA MAYS;

GENE EXPRESSION;

EMBRYOPHYTA; ESCHERICHIA COLI; ZEA; ZEA MAYS;

EID: 0036415771     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020509915672     Document Type: Article

References (29)

1. Armstrong C.L., Green C.E. and Phillips R.L. 1991. Development and availability of germplasm with high type II culture formation response. Maize Genet. Coop. Newsl. 65: 92-93.
2. Armstrong C.L. 1999. The first decade of maize transformation: A review and future perspective. Maydica 44: 101-109.
3. Austin S., Bingham E.T., Koegel R.G., Matthews D.E., Shahan M.N., Straub R.J. et al. 1994. An overview of a feasibility study for the production of industrial enzymes in transgenic alfalfa. Ann. NY Acad. Sci. 721: 234-244.
4. Bradford M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
5. Chikwamba R.K., Cunnick J., Hathaway D., McMurray J., Mason H. and Wang K. 2002. A functional antigen in a practical crop: LT-B producing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and Cholera toxin (CT). Transgenic. Res. (in press).
6. Christou P. 1996. Transformation technology. Trends Plant Sci. 1: 423-431.
7. Daniell H., Streatfield S.J. and Wycoff K. 2001. Medical molecular farming: Production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci. 6: 219-226.
8. Dickinson B.L. and Clements J.D. 1996. Use of Escherichia coli heat-labile enterotoxin as an oral adjuvant. In: Kiyono H., Ogra P.L. and McGhee (eds), Mucosal Vaccines. Academic Press, pp. 73-87.
9. Frame B.R., Zhang H., Cocciolone S.M., Sidorenko L.V., Dietrich C.R., Pegg S.E. et al. 2000. Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency. In Vitro Cell Dev. B. 36: 21-29.
10. Gallie D.R., Tanguay R.L. and Leathers V. 1995. The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation. Gene. 165: 233-238.
11. Gribskov M., Devereux J. and Burgess R.R. 1984. The codon preference plot: Graphic analysis of protein coding sequences and prediction of gene expression. Nucl. Acids Res. 12: 539-549.
12. Haq T.A., Mason H.S., Clements J.D. and Arntzen C.J. 1995. Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268: 714-716.
13. Hood E.E., Witcher D.R., Maddock S., Meyer T., Baszczynski C., Bailey M. et al. 1997. Commercial production of avidin from transgenic maize: Characterization of transformant, production, processing, extraction and purification. Mol. Breed. 3: 291-300.
14. Krebbers E., Bosch D. and Vandekekerekhove J. 1993. Prospects and progress in the production of foreign proteins and peptides in plants. In: Shewry P.R. and Gutteridge S. (eds), Plant Protein Engineering. Cambridge University Press, Cambridge, UK, pp. 315-325.
15. Marks M.D., Lindell J.S. and Larkins B.A. 1985. Quantitative analysis of the accumulation of zein mRNA during endosperm development. J. Biol. Chem. 260: 16445-16450.
16. Matzke A.M. and Matzke A.J.M. 1995. How and why do plants inactivate homologous transgenes? Plant Physiol. 107: 679-685.
17. Mason H.S., Haq T.A., Clements J.D. and Arntzen C.J. 1998. Edible vaccine protects mice against Escherichia coli heat labile enterotoxin (LT): Potatoes expressing a synthetic LT-B gene. Vaccine 16: 1336-1343.
18. Millar D.G., Hirst T. and Snider D.P. 2001. Escherichia coli heat labile enterotoxin B sub-unit is a more potent mucosal adjuvant than its closely related homologue, the B sub-unit of Cholera toxin. Infect. Immun. 69: 3476-3482.
19. Munro S. and Pelham H.R.B. 1987. A C-terminal signal prevents secretion of luminal ER proteins. Cell 48: 899-907.
20. Murray M.G. and Thompson W.F. 1980. Rapid isolation of high-molecular-weight plant DNA. Nucl. Acids Res. 8: 4321-4325.
21. Pen J., Sijmons P.C., Van Ooijen A.J.J. and Hockema A. 1993. Protein production in transgenic crops: Analysis of plant molecular farming. In: Industrial Crops Production. Elsevier, Amsterdam, pp. 241-250.
22. Rhee Y. and Staswick P.E. 1992. Nucleotide sequence for the soybean vegetative storage protein vspB gene. Plant Physiol. 98: 794-795.
23. Sambrook J., Frittsch E.F. and Maniatis T.H. (eds) 1989. Molecular Cloning, A Laboratory Manual. Chapters 9-31 to 9-62. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
24. Sangtong V., Mottl E.C., Long M.J., Lee M. and Scott M.P. 2001. Serial extraction of endosperm drillings (SEED)-A method for detecting transgenes and proteins in single viable maize kernels. (Protocols) Plant Mol. Biol. Rep. 19: 151-158.
25. Spangler B.D. 1992. Structure of Cholera toxin and the related Escherichia coli heat-labile enterotoxin. Microbiol. Rev. 56: 622-647.
26. Streatfield S.J., Jilka J.M., Hood E.E., Turner D.D., Bailey M.R., Mayor J.M. et al. 2001. Plant based vaccines: Unique advantages. Vaccine 19: 2742-2748.
27. Streatfield S.J., Mayor J.M., Barker D.K., Brooks C., Lampear B.J., Woodard S.L. et al. 2002. Development of an edible subunit vaccine in corn against entemtoxigenic strains of Eschericia coli. In Vitro Cell Dev. B. 38: 11-17.
28. Tacket C.O., Mason H.S., Lasonsky G., Clements J.D., Levine M.M. and Arntzen C.J. 1998. Immunogenicity in humans of a bacterial antigen delivered in a transgenic potato. Nature Med. 4: 607-609.
29. Zhong G.Y., Peterson D., Bailey M., Witcher D.R., Register J., Bond D. et al. 1999. Commercial production of aprotinin in transgenic maize seed. Mol. Breed. 5: 345-356.