Molecular characterization of DNA encoding 16S-23S rRNA intergenic spacer regions and 16S rRNA of pectolytic Erwinia species

Canadian Journal of Microbiology

Volumn 48, Issue 5, 2002, Pages 387-398

  Fessehaie, A ^a,b   De Boer, S H ^a,b   Levesque C A ^a,b  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b NONE

Author keywords

16S rDNA; Erwinia spp.; Intergenic spacer region; Phylogeny; tRNA genes

Indexed keywords

DNA; DNA SEQUENCES; RNA; GENES;

PHYLOGENETIC ANALYSIS; PHYLOGENETIC ANALYSES;

BACTERIA; MICROBIOLOGY;

DNA 16S; RNA 16S; RNA 23S; BACTERIAL DNA; BACTERIAL RNA; RIBOSOME RNA; SPACER DNA;

BACTERIUM; PHYLOGENY; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; ERWINIA; ERWINIA STEWARTII; MONOPHYLY; NONHUMAN; NUCLEOTIDE SEQUENCE; PANTOEA AGGLOMERANS; PECTOBACTERIUM CAROTOVORUM; PHYLOGENY; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TAXON; BACTERIAL GENE; GENE AMPLIFICATION; GENETIC CODE; POLYMERASE CHAIN REACTION; SEQUENCE DATABASE; SPECIES; SPECIES COMPARISON; SUBSPECIES;

BASE SEQUENCE; DNA, INTERGENIC; DNA, RIBOSOMAL; ERWINIA; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); ERWINIA; NEGIBACTERIA; PANTOEA AGGLOMERANS; PANTOEA STEWARTII SUBSP. STEWARTII; PECTOBACTERIUM CAROTOVORUM;

EID: 0036360788     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w02-026     Document Type: Article

References (44)

1. Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology
2. Sequence diversity in the 16S-23S intergenic spacer region (ISR) of the rRNA operons in representatives of the Escherichia coli ECOR collection
3. Intraspecific diversity of the 23S rRNA gene and the spacer region downstream in Escherichia coli
4. An Escherichia coli strain with all chromosomal rRNA operons inactivated: Complete exchange of rRNA genes between bacteria
5. Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region
6. Genetic diversity and host range in strains of Erwinia chrysanthemi
7. Deoxyribonucleic acid relatedness among erwinias and other enterobacteria
8. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
9. Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholera and Vibrio mimicus
10. Control of rRNA transcription in Escherichia coli
11. Isolation by genomic subtraction of DNA probes specific for Erwinia carotovora subsp. atroseptica
12. PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue
13. Evaluation of a PCR kit for the detection of Erwina carotovora subsp. atroseptica on potato tubers
14. Comparison of the small 16S to 23S intergenic spacer region (ISR) of the rRNA operons of some Escherichia coli strains of the ECOR collection and E. coli K-12
15. Inter- and intraspecies comparison of the 16S-23S rRNA operon intergenic spacer regions of six Listeria spp.
16. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region
17. A compilation of large subunit RNA sequences presented in a structural format
18. Lessons from an evolving rRNA: 16S and 23S structures from a comparative perspective
19. Phylogenetic position of phytopathogens within the Enterobacteriaceae
20. Identification of Erwinia carotovora subsp. atroseptica with a non-radioactive DNA probe
21. Characterization of Erwinia carotovora subspecies and detection of Erwinia carotovora subsp. atroseptica in potato plants, soil and water extracts with PCR-based methods
22. Improved PCR detection sensitivity of Erwinia carotovora subsp. atroseptica in tuber peel extract by prior enrichment on a selective medium
23. Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms
24. Erwinia pyrifoliae sp.nov., a novel pathogen affecting Asian pear trees (Pyrus pyrifolia Nakai)
25. Phylogenetic analysis of Erwinia species based on 16S rRNA gene sequences
26. 16S/23S sequencing
27. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny
28. Selection of polymerase reaction primers from an RNA region for specific detection of Clavibacter michiganensis subsp. sepedonicus
29. The three Ds of PCR-based genomic analysis of phytobacteria: Diversity, detection, and disease diagnosis
30. Variation of the ribosomal operon 16S-23S gene spacer region in representatives of Salmonella enterica subspecies
31. Differentiation of Erwinia carotovora subsp. atroseptica and carotovora by RAPD-PCR
32. Ribotyping of Erwinia chrysanthemi strains in relation to their pathogenic and geographic distribution
33. Characterization of Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR-amplified fragments of pel genes
34. Anwendung der polymrease-kettenreaktion (PCR) zum nachweis von Erwinia carotovora subsp. atroseptica, dem erreger der schwarzbeiningkeit und Nassfäule bei kartoffeln
35. Identification of Erwinia carotovora from soft rot diseased plants by random amplified polymorphic DNA (RAPD) analysis
36. Genome data shake tree of life
37. Detection of Erwinia carotovora subsp. atroseptica and E. chrysanthemi in potato tubers using polymerase chain reaction
38. Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR
39. Rapid identification and differentiation of the soft rot erwinias by 16S-23S intergenic transcribed spacer-PCR and restriction fragment length polymorphism analyses
40. Specific detection of Erwinia carotovora subsp. atroseptica with a digoxigenin-labeled DNA probe
41. Bandstab: A PCR-based alternative to cloning PCR products